The author reviews the main characteristics and uses of a number of devices and automated systems for the examination of the gastrointestinal tract (GIT). Shows the main principles of designing the automated working places of the gastroenterologists (APM-B "Gastro") and their uses at the diagnostic centres. Provides examples of some investigations of the GIT.

Diagnosis, Computer-Assisted↗

PubMedDate not supplied

[A quantitative evaluation of the changes in the proliferation of a human lymphocyte culture after mutagen exposure at the G0 stage].

Cell cycle delay of human lymphocytes treated with mutagens at G0 stage has been studied using a mathematical model. Cell cycle delay depends on entry cells into the first S phase and is independent on the cell cycle duration.

Aziridines↗

PubMedDate not supplied

[A mathematical model of the proliferative kinetics of cells labelled with brominated DNA precursors].

A mathematical model of cell division is presented. It permits analyzing cell proliferation obtained by using BrdU. Numerical experiments show that this model adequately describes experimental data. Application of this model permits decreasing experimental data bulk.

Animals↗

PubMedDate not supplied

[Changes in the number of sister chromatid exchanges in cultured Chinese hamster cells with limited proliferation. Additional studies].

During "stationary phase ageing" of cultured Chinese hamster cells (B11dii-FAF28 line, 2372a clone), i. e. while decreasing the proliferation rate and in the stationary growth phase the frequency of spontaneous sister chromatid exchanges (SCE) progressively increases (from 2- to 23-day "age"); the frequency of thiophosphamide-induced (1h) SCE increases from 2- to 23-day "age" by the same value as the frequency of spontaneous SCE; the cells deepen into the R-phase of the cell cycle.

Animals↗

PubMedDate not supplied

[The duration of cell cycle phases studied using 5-bromodeoxycytidine].

The quantitative method is suggested to estimate cell cycle phase durations and dispersions of progress through the phases for population of cells. The method is based on the analysis of frequency of cells with different staining of sister chromatids by means of 5-bromodeoxycytidine. The process of cell population progress is described by the Gauss probability integral. The durations of the cell cycle phases are determined for cell culture of Chinese hamster.

Animals↗

PubMedDate not supplied

[Automated system for research on intragastric pH measurement].

An automated system for the study of the intragastric PH-metry on the basis of the Electronika KI-20 microcomputer has been considered. The paper gives a brief description of the apparatus, program and the operator's work with the system.

Equipment Design↗

PubMedDate not supplied

[Comparison of chromosome aberrations, sister chromatid exchanges and unscheduled DNA synthesis in the evaluation of the mutagenicity of environmental factors].

Mutagenic character of formaldehyde in vivo was estimated by determining the level of chromosomal aberrations, sister chromatid exchanges and unscheduled DNA synthesis in human lymphocytes. It was found that in case of occupational exposure to formaldehyde the unscheduled DNA synthesis after thiophosphamide treatment in vitro was inhibited and spontaneous level of chromosomal aberrations increased. A negative correlation observed between the unscheduled DNA synthesis and sister chromatid exchanges indirectly confirmed a connection of these exchanges with the DNA repair. The comparison of the results obtained from evaluation of chromosomal aberrations, sister chromatid exchanges and unscheduled DNA synthesis permits suggesting that these methods estimate different sides of the mutagen interaction with a cell and should be considered as mutually complementary methods but not as interchangeable ones.

Air Pollutants, Occupational↗

PubMedDate not supplied

[Changes in the level of sister chromatid exchanges in cultured Chinese hamster cells undergoing limited proliferation].

"Stationary phase ageing" of cultured Chinese hamster cells (when proliferation rate decreases and in the stationary growth phase) produces an increase in the frequency of spontaneous sister chromatid exchanges (SCE). Thiophosphamide-induced (24 h) frequency of SCE increases from 2-day to 5-day "age" and later (in the stationary phase) is practically the same. The "stationary ageing" cultured cells are suggested to be used as a model system for studying molecular-genetic age changes.

Animals↗