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A N Gol'tsev

Publications and source records attributed to A N Gol'tsev.

6 recordsLinked to original sources
PubMed1985

[Conservation of bone marrow CFUc in different phases of the cell cycle during cryopreservation].

The effect of low temperature (-196 degrees C) preservation on the recovery of colon-forming units (CFUs) of bone marrow at different phases of the cell cycle before cryopreservation is dealt with. The intact bone marrow "enriched" with CFUs in S phase of the cell cycle and the bone marrow without colony-forming units in S phase were exposed to cryopreservation. After cryopreservation of the bone marrow enriched with CFUs in S phase and th bone marrow without colony-forming units in S phase the number of CFUs decreases by the same value as in the cryopreserved bone marrow obtained from intact mice.

Animals↗
PubMed1982

[Comparative study of the proliferative activity of native and cryopreserved colony-forming units in humans and mice].

The agar culture method was used to state that colony-forming units responsible for cell structures of a hemopoietic series remained undamaged after cryopreservation (-196 degrees) in a suspension of mouse and human donor bone marrow. Cultivation of cryopreserved bone marrow revealed the inhibition of proliferative activity of cells growing in agar as compared with native ones. The inhibition of DNA synthesis was the basis of this process.

Animals↗
PubMed1980

[Cellular structures formed during bone marrow cultivation in agar].

The growth-enhancing effect and the differentiation of colonies formed by native and frozen-thawed myelokaryocytes in semiliquid agar were observed over a period from the 2nd to the 28th day. The cultivation of bone marrow in agar was shown to form colonies consisting of haemopoietic cells as well as of stromal elements. Under the cultivation of frozen-thawed bone marrow, the retardation of proliferative activity of growing cells was observed within up to 4 days. Later on the proliferative character and the rate of the myelokaryocytes of both the types were seen identical. The question of a possible existence of haemopoietic tissue and stromal elements in the progenitor cells of normal bone marrow is being discussed.

Agar↗
PubMedDate not supplied

[Effect of PHA on lymphocyte respiratory activity in mice].

A study of the intensity of oxygen consumption by mice lymphocytes at different stages of cultivation with PHA showed a pronounced increase of the respiratory activity beginning from the 14th hour. Maximum values of oxygen consumption by stimulated cells were observed at the 16th and 30th hours of cultivation (before the beginning of blastogenesis and before the maximum uptake of H3-thymidine, respectively.

Animals↗
PubMedDate not supplied

[Effect of low-temperature preservation on the proliferative activity and topography of the dissemination of donor and cadaveric colony-forming units (CFU) in an irradiated organism].

Investigation carried out by means of chromosome marker T6T6 showed that frozen donor and cadaver bone marrow cells (6 h and 12 h after death) survive and proliferate in the organism of lethally irradiated recipient; the proliferation and differentiation intensity of cadaver cells is reduced in comparison with the donor cells.

Animals↗