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A N Petrova

Publications and source records attributed to A N Petrova.

At least 19 recordsLinked to original sources
PubMed1991

[An active 2.5S RNA-polysaccharide complex].

The information on the initial step of enzymatic reaction of 2.5S RNA isolated from muscle 1,4-alpha-glucane branching enzyme (EC 2.4.1.18), isomerase amylose, has been reported for the first time. The 2.5S RNA-polysaccharide (starch) complex was isolated and partly characterized. It was shown that the complex retains its integrity during precipitation with ethanol, gel-filtration on a Biogel P-150 column and electrophoresis but dissociates into RNA and starch upon fractionation on a DEAE-52 cellulose column. Treatment of the original preparation with RNAase fully reflects the complex formation: with a decrease in the RNA content in the original preparation that of the synthesized complex diminishes. The RNA-polysaccharide complex exhibits the properties of the branching enzyme; its enzymatic activity markedly exceeds that of the free RNA.

1,4-alpha-Glucan Branching Enzyme↗
PubMed1979

Primary structure of the nucleic acid from the 1,4-alpha-glucan branching enzyme.

The primary structure of the nucleic acid from the branching enzyme 1,4-alpha-D-glucan: 1,4-alpha-D-glucan 6-alpha-(1,4-alpha-glucano)-transferase (2.5-S RNA) isolated from rabbit muscles has been elucidated. The polyribonucleotide consists of 31 nucleotides; the unique features of the polyribonucleotide are the unusually high content of modified nucleotides (32%) and guanine residues (40%). Apparently 2.5-S RNA belongs to a class of nucleic acids unknown up to now. It is the first time that the structure of a nucleic acid component from a ribonucleoenzyme has been defined. This work is a preprequisite for gaining insight into the intimate activating effect of the poly-ribonucleotide on the enzyme action.

1,4-alpha-Glucan Branching Enzyme↗