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Biomedical subjects

A N Shenderov

Publications and source records attributed to A N Shenderov.

5 recordsLinked to original sources
PubMed1988

[Comparative restriction analysis of chromosomal DNA of strains of Photobacterium leiognathi].

Chromosomal DNA in 5 hereditary variants occurring in Photobacterium leiognathi population was subjected to restriction analysis. The variants differed in the levels and regulation of luminescence and colony morphology. Agarose electrophoresis of DNA fragments isolated after exposure to Hind II, Bam HI, Bgl I and Pst I restriction endonucleases revealed respectively 38, 28, 35 and 29 fragments equally distributed by their molecular weights. Electrophoregrams of the 5 strains were absolutely identical. After exposure of DNA of all the strains to PVu II, Xho II, Sal GI and Eco RI restriction endonucleases there were detected no fragments. The pleoiotropic genetic variation in these strains was not associated with large deletions or amplification of chromosomal DNA regions.

Chromosomes, Bacterial↗
PubMed1988

[Cloning and insertion mutagenesis of DNA fragment coding for the luminescent system of Photobacterium leiognathi].

Fragments of DNA, obtained from the luminescent bacterium Photobacterium leiognathi and inserted into the plasmid pBR322, were found to code for the luminescence expressed in E. coli cells. The genetic functions necessary for light production in E. coli are localized on a DNA fragment of about 7 kbp. The insertion mutagenesis was used to define the luminescence functions encoded by the hybrid plasmid.

Cloning, Molecular↗
PubMed1979

[Genetic studies of Photobacterium mandapamensis. II. The classification of mutants with an altered luminescence intensity according to their sensitivity to exogenous aldehyde].

The collection of 157 dark and dim Photobacterium mandapamensis strains was divided into four groups using the addition of 0.2 ml of 0.15% myristis aldehyde to cell suspension. The luminescence did not change in the presence of the aldehyde in 76 mutants, it decreased in 30 mutants, it was 2--8-fold increased in 35 mutants, and it was increased more than 10-fold in 16 mutant strains. 19 strains of those having luminescence in the presence of the aldehyde have mutations in genes controlling the biosynthesis of the aldehyde factor. Among them mutants are chosen which can be used as indicators for the aldehyde presence in the medium.

Aldehydes↗
PubMed1978

[Isolation of bacterial luminescence reaction inhibitor from Photobacterium sp. cells].

The factor having a strong inhibitory effect on bacterial luminescence was isolated from the luminous bacteria species Photobacterium sp. The inhibitor purified by gel filtration on the biogel and by DEAE chromatography was homogenous (single bound during electrophoresis in polyacrylamide gel), it reacted with coomassie brilliant blue and gave a positive Lowry reaction on protein. Molecular weight was about 30,000 as determined by SDS-polyacrylamide gel electrophoresis. The absorption spectrum was characterised by the maximum at 209 nm and unexpressed maximum in the region of 260 nm. It was shown that the inhibitor had an efficient inhibitory effect on both partially- and highly purified luciferase preparations from different species of luminous bacteria, but it produced no effect on the activity of specific NADH: FMN-oxidoreductase.

Bacterial Proteins↗