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Biomedical subjects

A N Shutko

Publications and source records attributed to A N Shutko.

At least 19 recordsLinked to original sources
PubMed1991

[Physicotechnical and dosimetric support of human whole-body irradiation in a standard medical source of photon radiation].

The paper is concerned with physicotechnical and dosimetric support of technology of therapeutic whole-body irradiation on a serial gamma apparatus ROKUS, with which a majority of radiological institutions in the USSR are fit out. Technology consists of the following procedures: patient positioning with relation to a source with a horizontal axis of radiation. Correction of dose distribution by means of a three-element compensator taking into account patient's constitutional peculiarities and preradiation preparation data; double irradiation in the bilateral regimen in the supine position.

Filtration↗
PubMed1989

[Changes in primordial lymphoid organs in chronic glomerulonephritis].

Changes in primordial lymphoid organs (bone marrow and thymus) with respect to the content of a serum thymic factor in the blood and specific activity of end desoxynucleotidyltransferase in the bone marrow and peripheral blood were studied in patients with chronic glomerulonephritis with and without the nephrotic syndrome. The mechanisms of autoimmunization were also studied with respect to the presence of renal tissue autoantigen antibodies. Alteration of primordial lymphoid organs was not attended by the autoimmune changes, i.e. production of the renal autoantigen antibodies was not increased. This makes difficult interpretation of the nephrotic syndrome as an autoimmune process and of glomerulonephritis as a whole. Lymphocytes bearing end desoxynucleotidyltransferase more probably participate in the developmental mechanisms of chronic glomerulonephritis.

Bone Marrow↗
PubMed1983

[Role of terminal deoxyribonucleotidyl transferase in stimulating lymphocyte DNA synthesis as affected by PHA].

3H-thymidine incorporation was measured in DNA of the human lymphocytes from the peripheral blood after its PHA-stimulation in vitro. The increased PHA-stimulation was established after addition to the medium of exogenous terminal deoxynucleotidyl transferase (TDT) for cultures with small level of PHA-stimulation only. TDT without PHA exerted no stimulating action on the culture of lymphocytes.

Cells, Cultured↗
PubMed1979

[Biological activity of exogenous DNA in rat thymocytes].

The thymocyte's viability and hybridization of one fraction thymocyte RNA with exogenous DNA have been studied after the incubation of cells with this DNA. The exogenous DNA decreases the number of viable cells and increases the hybridization percentage of pulse-labeled RNA. The above changes failed to be induced after the incubation of these cells with the complex DNA with actinomycin D, despite the fact that this complex penetrates into the cells as easily as does the free DNA.

Animals↗
PubMed1976

[Mechanism of functional adaptation of intestinal epithelial cells].

Using preparations of exogenous DNA and products of its in complete degradation, the causes leading to the compensatory changes of the share of membrane hydrolysus in the total invertase activity of the irradiated rat's intestinal epithelial were analysed experimentally. It is shown that the factor stimulating the enzyme redistribution towards the brush border zone is not the amount of mature villous cells, but the level of mitotic activity in crypts.

Animals↗
PubMed1976

[Cell response to penetration by informational macromolecules].

A relation is shown between the kinetic for of DNA uptake by the thymocytes (extremal or monotonous), the level of uptake (low or high) and the origin of DNA (hetero-or homologous). Preliminary irradiation of cells promotes a manifestation of a cytopathogenous effect of the heterologous DNA. The association between the cytopathogenous effect of DNA and that of its information characteristics has been confirmed. The demonstration of cytopathogenous effects of native and degraded DNA was studied as a function of time of their storage. The chromatographic analysis of dispersion of the molecular weights of preparations at different time of their starage allowed a conclusion of the important role played by structure defects in manifestation of cytopathogenous properties of DNA.

Animals↗
PubMed1976

[The role of the autonomic nervous system in regulating the hydrodynamics of the intact eye].

In cats, the low-molecular indicator: radioactive phosphorus, and the tonography revealed that the local application of acetylcholine accelerates the fluid exchange in the intact eye, considerably facilitating the outflow. Noradrenaline and the threshold stimulation of the cervical sympathetic nerve slow down the intraeye fluid exchange, decreasing the outflow. The data obtained suggest that the neural regulation of the eye hydrodynamics involves, mainly, a direct effect of the vegetative innervation on the eye drainage system.

Acetylcholine↗
PubMed1976

[Transport of heterologous DNA in normal and tumor cells and its modification by radiation].

The authors have studied in a comparative way the kinetics of incorporation of labelled heterologous DNA in tumor and nontumor cells of an identical histological type, obtained from non-irradiated and irradiated with 800 rad rats. It has been shown that the process of incorporation of heterologous high-polymer DNA in cells is somewhat undulatory, and its temporal characteristics and level -- tissue-specific. Difference between tumor cells and their normal prototype show a quantitative character, conditioned by a large surface area. 30 minutes following the irradiation qualitative changes opposite for both kinds of cells were observed in the kinetics of incorporation: an acceleration for lymphoyctes and retardation for Pliss lymphosarcoma and Zajdela hepatoma cells.

Animals↗