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A Nagashima

Publications and source records attributed to A Nagashima.

79 records · Page 5Linked to original sources
PubMed1984

Antitumor activity of macrophages in lung cancer patients with special reference to location of macrophages.

Antitumor activity of macrophages from the peripheral blood, pleural cavity, and alveoli of 35 patients with primary lung cancer was examined. Cytostatic activities of peripheral blood monocytes and alveolar macrophages from either tumor-bearing or non-tumor-bearing segments declined in association with metastasis to regional lymph nodes, an increase in tumor size, and the development of pleural invasion. However, no such correlation could be observed between the cytostatic activity of pleural cavity macrophages and the degree of pleural invasion. The cytostatic activity of pleural cavity macrophages was found to be suppressed when the pleural invasion extended beyond the visceral pleura to the neighboring lobe or chest wall. On the other hand, the cytostatic activity of pleural cavity macrophages was markedly augmented when pleural invasion was limited to within the visceral pleura, although it was low in patients with no visceral pleural invasion. These results suggest that the pleural cavity is isolated from sites of systemic immunological response and that systemic immunological response does not strongly affect pleural cavity macrophages.

Cell Line↗
PubMed1981

Effect of decreased plasma colloid osmotic pressure on development of pulmonary edema in dogs.

The effect of decreased plasma colloid osmotic pressure on the development of pulmonary edema was studied in anesthetized dogs. Lung lymph flow was used aa a sensitive and reliable indicator of fluid filtration rate in the lung. When plasma colloid osmotic pressure alone was reduced by slow infusion of saline, and hydrostatic pressure in the pulmonary vascular bed was maintained at normal level by exsanguination, lung lymph flow increased almost linearly with the reduction in colloid osmotic pressure, but was not increased more than five fold of the control, despite a reduction of 80% in the plasma colloid osmotic pressure. Furthermore, there was no evidence of fluid in the tracheal aspiration and no gross evidence of pulmonary edema. In contrast, both decrease in colloid osmotic pressure and increase in pulmonary capillary hydrostatic pressure produced a marked increase in lung lymph flow. This flow varied linearly with the level of the pulmonary artery wedge-plasma colloid osmotic pressure difference and approached twelve fold of the control, when the plasma colloid osmotic pressure was reduced by 73% and the pulmonary artery wedge pressure was elevated by 20 mmHg from the baseline. Our data indicate that decreased colloid osmotic pressure is not associated with the development of pulmonary edema, when there is no increase in pulmonary vascular hydrostatic pressure.

Animals↗
PubMedDate not supplied

A hybrid artificial esophagus using cultured human esophageal epithelial cells.

It is important to cover an artificial esophagus with epithelium to prevent stenosis and reinforce the lumen. We examined the possibility of epithelialization of the surface of a latissimus dorsi muscle of athymic mice using cultured human esophageal epithelial cells. Normal human esophageal mucosa (0.5 cm2) was processed from specimens resected from patients with esophageal cancer, and epithelial cells were cultured on collagen gel in a culture dish (30 cm2) for about 10 days. The collagen sheets with cultured cells were transplanted onto the surface of latissimus dorsi muscles of 16 athymic mice. Four animals were killed 4, 8, 12, and 16 days after transplantation, and the transplanted collagen sheets were studied histologically. Cultured sheets had attached to the muscle in all 16 athymic mice. By 16 days, neovascularization in the collagen layer was observed and the epithelial cell layer was similar to normal human esophageal epithelium. This study suggests that epithelialization on latissimus dorsi muscle is possible using cultured human esophageal epithelial cells, and this could lead to the practical use of a hybrid artificial esophagus consisting of a latissimus dorsi muscle tube with its inner side epithelialized by cultured cells.

Animals↗