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Biomedical subjects

A Neufahrt

Publications and source records attributed to A Neufahrt.

7 recordsLinked to original sources
PubMed1978

Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). II. Viscosity changes after enzyme treatment.

Human skin epithelial-like cells (NCTC strain 2544) were grown in NCTC 135 medium. Neuraminidase and hyaluronidase were added to the growth medium. Cells were incubated 96 h at 36 degrees C. Growth rate and viscosity of cell suspensions were measured after forming single cells mechanically (mopping). With addition of neuraminidase and hyaluronidase, respectively, the growth rate remains unchanged. With neuraminidase a distinct raise in viscosity was achieved, whereas with hyaluronidase only a small effect was seen. The characteristic structure viscosity is maintained in all forms of the viscosity curves at different shear-rates.

Animals↗
PubMed1977

Disc-electrophoretic separation of water soluble proteins from scales of different forms of ichthyosis.

In extracts of scales of different forms of ichthyosis, disc-electrophoretic separation of water soluble proteins was performed. Number and position of the protein bands correspond with number and position of the bands of extracts of normal keratin and psoriatic scales. However, in comparison to normal keratin, all examined forms of ichthyosis showed in zone II the enriched bands Nos. 6 and 7. This permits a distinct differentiation from psoriasis in which in zone II the bands Nos. 9 and 10 are enriched. In congenital ichthyosiform erythroderma and in ichthyosis combined with atopic dermatitis, in zone III the bands, containing gamma globulins, are enriched as an expression of the concomittant exudative process. The protein content of scale extracts of ichthyosis is 2 to 5 times lower than the one of psoriasis.

Dermatitis, Atopic↗
PubMed1976

Long-term tissue culture of epithelial-like cells from human skin (NCTC strain 2544). I. Measurement of viscosity.

Viscosities of cell suspensions of human skin epithelium (NCTC strain 2544) were determined by a Wells-Brookfield rotation viscosimeter. A structure viscosity may be postulated by the form of the viscosity curves at different shear-rates. At a cell number of 0.5 X 10(6) cells/ml a viscosity of 1.094 centipoise (25degrees C, shear-rate 230 s-1) could be found. Since a suspension of single cells may be easily formed mechanically (mopping) the NCTC 2544 cells are useful as investigational model regarding the investigation of membrane characteristics.

Cell Membrane↗
PubMed1975

Subcellular distribution of phosphatases, proteinases, and ribonucleases in normal human stratum corneum and psoriatic scales.

The subcellular distribution of phosphatases, proteinases, and ribonucleases of normal human stratum corneum and psoriatic scales was determined after differential centrifugation. All psoriatic enzymes showed much increased activities as compared to the normal stratum corneum enzymes. The highest activities of alkaline phosphatase from psoriatic scales could be detected in the nuclear fraction. The main activities of all other tested phosphatases and proteinases were present in the cytoplasmatic fraction. The subcellular distribution of the ribonucleases varied according to the pH value.

Alkaline Phosphatase↗
PubMed1975

[Isolation and amino acid composition of two pathologically augmented proteins from psoriatic scales (author's transl)].

Two protein bands of water soluble extracts of psoriatic scales, which appear intensified in disc-electrophoretical separation, were isolated and their amino acid compositions were determined. It could be demonstrated that band 9 was rich of glycine, alanine, aspartic acid, leucine, and serine and that band 10 was rich of glycine, threonine, and serine. Both proteins contained an amino-sugar (galactosamine).

Alanine↗