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Biomedical subjects

A Ohyama

Publications and source records attributed to A Ohyama.

At least 19 recordsLinked to original sources
PubMed1992

Effect of ciclosporin on cardiac output and regional blood flow in rats: ciclosporin-induced nephropathy and its prevention with calcium channel blocker.

The effect of ciclosporin (CS) on cardiac output (CO) and regional blood flow was studied using the microsphere method in heminephrectomized rats with and without renal arterial clamping prior to the administration of CS. The effect of a calcium (Ca) channel blocker, verapamil, was also examined on CS-induced nephropathy. CS at a dose of 40 mg/kg per day was given orally using a gastric tube for 7 days. Verapamil was given in the drinking water for 7 days. Significant increases in blood urea nitrogen (BUN) and serum creatinine (sCr) with a significant decrease in renal inulin clearance (CIn) were noted after 7 days of CS administration in both intact and ischemic-kidney groups, indicating the development of CS-induced nephropathy. The ischemic-kidney group showed a significantly severe nephropathy as compared with the intact-kidney group. As for change in CO and regional blood flow, CS caused a significant decrease in CO, renal blood flow (RBF) and brain blood flow, while hepatic arterial blood flow and muscular blood flow significantly increased. The renal outer cortical blood flow decreased markedly while the inner cortical blood flow remained unchanged. Although verapamil slightly but significantly decreased mean arterial blood pressure in CS-treated rats, CO and its distribution did not change. BUN and sCr were not significantly ameliorated in the intact-kidney group. However, in the ischemic-kidney group, verapamil caused a significant improvement in RBF, ameliorating CS-induced elevation of BUN and sCr, and a decrease in CIn.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1992

Application of two-color immunofluorescence staining to demonstration of T-cells and HLA-DR-bearing cells in rheumatoid synovitis.

We studied the localization of T-cells and HLA-DR antigen-bearing (DR+) cells in rheumatoid synovitis by employing an improved two-color immunofluorescent staining (TCIF) technique. With this technique we have successfully identified DR+ activated T-cells in the inflammatory synovium. T-cells expressed HLA-DR antigen when they were in contact with DR+ antigen-presenting cells (APC). In addition, activated T-cells showed characteristic distribution within the synovium: they were found around high endothelial venules, within lymphoid follicles, and in hyperplastic synovial lining, suggesting their involvement in the development of rheumatoid synovial lesions via interaction with synovial DR+ APC lineage cells. These findings may contribute to better understanding of the role of activated T-cells in the histogenesis of rheumatoid synovitis, a typical chronic inflammatory lesion.

Arthritis, Rheumatoid↗
PubMed1992

A novel cDNA clone for acid invertase in tomato fruit.

The sequence of a novel cDNA clone, Aiv-1, for tomato acid invertase was similar to that of TIV1 (Klann et al., 1992) for the enzyme except for a unique intron-like insertion. It is considered that Aiv-1 is derived from either an alternatively spliced mRNA for an isozyme or a pre-mRNA of TIV1.

Amino Acid Sequence↗
PubMed1991

Expression of decay-accelerating factor is reduced on hyperplastic synovial lining cells in rheumatoid synovitis.

Decay-accelerating factor (DAF), a membrane inhibitor of homologous complement activation, is present in synovial cells lining joint space and detected in synovial fluid. DAF is considered to protect synovial membrane from complement-mediated injury associated with articular inflammation. We studied the immunohistopathological features of DAF molecules in synovial membrane of rheumatoid synovitis using a DAF-specific monoclonal antibody, 1C6. Reacting molecules with the 1C6 antibodies in synovial tissue extracts formed a 70-kD band in Western blot analysis. DAF was strongly detected on the flat synovial lining cells, but weakly on the hyperplastic and multi-layered lining cells in rheumatoid synovitis. The latter cells reacted with anti-Leu-M3 antibodies specific for a cell surface marker of activated macrophages, sometimes accompanied by C3 and IgM deposition on the superficial synovial membrane. These results suggest that active rheumatoid synovitis characteristically with hyperplastic synovial lining cells is out of control by DAF, thereby permitting further complement-mediated injury.

Antibodies, Monoclonal↗
PubMed1991

Examination of aggravating factors of urinary excretion of N-acetyl-beta-D-glucosaminidase after extracorporeal shock wave lithotripsy.

We measured urinary N-acetyl-beta-D-glucosaminidase (NAG) excretion before and after extracorporeal shock wave lithotripsy (ESWL) with a view to study the underlying factors which aggravate renal impairment immediately after ESWL. The factors associated with the significant elevation of urinary NAG after ESWL included female sex, a previous history of ESWL, and urinary tract infection (UTI). By studying the backgrounds of these factors, we found that both the female group and the group with a previous history of ESWL contained significantly more patients with UTI. These findings indicate that UTI may be one of the risk factors which aggravate renal damage immediately after ESWL, and that careful management is necessary in the ESWL treatment of urinary tract stones with UTI.

Acetylglucosaminidase↗
PubMed1991

[Effects of verapamil on cyclosporine. A (CsA)-induced nephropathy in ischemic kidney model in rats: changes in systemic hemodynamics and hepatic and renal microsomal cytochrome P-450].

We have examined the effect of verapamil on CsA-induced nephropathy by measurement of systemic hemodynamics including each organ blood flow using the microsphere method in ischemic kidney model of hemi-nephrectomized Wistar rats. Hepatic and renal microsomal cytochrome P-450 contents and their enzyme activities were measured to study the correlation between CsA-induced nephropathy and induction of hepatic and renal microsomal cytochrome P-450. All rats were hemi-nephrectomized (l-nephrectomy) and were classified into the following 6 groups: 1) control groups, 2) CsA at a dose of 40 mg/kg per day orally for 7 days (CsA group), 3) Oral administration of verapamil for 7 days in the CsA group (CsA + V group), 4) 20 min clamping of the remaining right kidney pedicle (Ischemic, Is group), 5) CsA was administered in the Is group (Is + CsA group), 6) Addition of verapamil to CsA in the Is + CsA group (Is + CsA + V group). Verapamil was given in the drinking water and the average dose calculated from the amount of drinking was 4.7 +/- 1.0 mg/kg per day and 5.2 +/- 0.7 mg/kg per day for the CsA + V group and the Is + CsA + V group, respectively. CsA caused significant increases in BUN and serum creatinine (sCr) with a significant decreases in renal inulin clearance (CIn) in all groups. When compared with the Is group, CsA caused significant decreases in cardiac output and all organ blood flow especially in renal blood flow with significant increases in BUN and sCr in the Is + CsA group.2+ degree of nephropathy.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1991

[Experience of extracorporeal shock-wave lithotripsy for the urolithiasis in horseshoe kidney].

At Osaka City University, 1,987 patients with urolithiasis have been treated by extra-corporeal shockwave lithotripsy (ESWL) during a four-year period. We treated 5 patients with horseshoe kidney and the obtained results were analyzed retrospectively. Three of these patients, who had a solitary stone could successfully be treated by ESWL as monotherapy. One who had multiple stones required transurethral lithotripsy after ESWL due to stone-street. The remaining 1 patient who had undergone heminephrectomy developed perirenal hematoma after ESWL, which spontaneously disappeared without any specific treatment. We discuss the special care related to the use of ESWL in the horseshoe kidney.

Adult↗
PubMed1991

[Case report: a foreign body (gauze) in the bladder].

A case of bladder tamponade caused by a foreign body (gauze) in the bladder of a 24-year-old man is reported. He had been treated with the Lambotte wire and screw to repair his pubic bone fracture caused by a traffic accident. About one year later, the wire and screw were removed from the patient. However, he suffered from gross hematuria and bladder tamponade on the 11th day after the 2nd operation, and he was transferred to our outpatient department on emergency. Further urological examinations were done for this case. The patient was diagnosed as having bladder tamponade caused by a foreign body, a gauze, in the bladder. Among these examinations NMR-CT gave us the most interesting and useful findings. NMR-CT showed that the gauze penetrated the bladder wall.

Adult↗
PubMed1990

[Endophthalmitis induced by herpes simplex virus, a study on early phase].

We studied the early phase of herpetic endophthalmitis to clarify the process of necrotizing retinitis by herpes simplex virus type-1 (HSV-1) and viral localization in the retina. A clone, isolated from HSV-1 Miyama for its high yield on Vero cells, was inoculated on mono-layers of Vero cells. Two days after inoculation, the cells were frozen-and-thawed once and were centrifuged. The supernatant was diluted with Hanks' balanced saline solution. The viral suspension 1.5 x 10(5) pfu/ml) was injected intravitreally to colored rabbits for 0.1 ml. All rabbits showed exudative iritis from 1 to 2 days after inoculation, vitreal opacities from 2 to 4 days and retinal exudates from 2 to 6 days. These exudates initially considered of necrotic materials of the inner nuclear layer. These fused with one another gradually and formed massive exudates which spread to the whole retinal layer, causing retinal necrosis. Viral particles were found in the nuclei of the inner nuclear layer at first, and later in the nuclei of Müller cells, ganglion cells and axon of the nerve fiber. These findings indicated that retinal lesion occurred primarily in viral replication of the inner nuclear layer.

Animals↗
PubMed1990

[An immunoperoxidase study of early phase experimental retinitis induced by herpes simplex virus].

Herpes simplex virus type 1 (HSV-1) was intravitreally inoculated in domesticated rabbits, and retinitis was observed with the ABC-method using peroxidase-conjugated antibody. Trypsin digestion was used for antigen visualization. Three days after inoculation, although cell degeneration was not found in the retina, DAB was deposited on the inner limiting membrane and the nerve fiber layer, which corresponded to the basal side of Müller cells. These findings indicated that viral antigen was already present in the retina at this early stage. Four or five days after inoculation, numerous variously sized exudative retinal lesions were seen. In the small exudative foci, DAB deposition was restricted to only in the nuclei of the nerve cells of the inner nuclear layer, while it extended to the outer and inner retinal layer in the large exudative foci. These results suggested that when HSV-1 was injected intravitreally it invaded from the basal side of Müller cells to the inner nuclear layer, where it replicated. The virus spread to the outer nuclear layer and ganglion cells as infection proceeded.

Animals↗
PubMed1990

[Optic nerve finding in experimental retinitis induced by herpes simplex virus].

We reported histological changes and viral localization of HSV-1 in the optic nerve by the ABC-method and electron microscopy, after intravitreal inoculation. There was neither histological change nor antigen deposition on the specimen of 3 days after inoculation. Some series of antigen-positive sites were successively found along the nerve fiber from the lamina cribrosa to the optic chiasma in the optic nerve on 4 days after inoculation. Round cell infiltration were also found around these sites, and these changes increased on the 8th day. Antigen-positive cells were mainly considered to be oligodendroglia and astrocytes. These results suggested that HSV-1 infected the optic nerve fiber via the retina. On the way to be the brain by axonal transport, they would infiltrate to the glial cells, where they could replicate and spread to neighbouring glial cells.

Animals↗
PubMed1990

[Evaluation of the results of extracorporeal shock-wave lithotripsy (ESWL) for solitary upper urinary tract stone].

At Osaka City University Hospital, we performed extracorporeal shock-wave lithotripsy (ESWL) for 1277 patients in a total of 1788 sessions using Dornier kidney lithotripter Model HM III from July, 1985 to the end of December, 1988. Among the patients with a solitary stone, 911 cases were available for the follow-up study and we have compared the results among these cases in respect to the stone location and size. The results obtained were as follows: the ratio of kidney stone to ureter stone was found to be 2.2:1 in male, and 3.8:1 in female patients. This indicates that ureter stones are more frequently found in male than in female patients. In addition, we compared the size of kidney stones found in the patients including 415 male and 243 female patients. Stones larger than 20 mm were more frequently found in female than in male patients. Moreover, a stone composition study of these patients showed that the contents of both phosphate calcium and magnesium ammonium phosphate were higher in female than in male patients. ESWL performed against stones at pelvis and calyces exhibited the best results. The results obtained with the stones less than 20 mm in diameter were especially favorable with a success rate of 100% for the stones less than 10 mm and 83% for the stones 10-20 mm, in diameter, while the results with the stones larger than 20 mm in diameter were rather poor with a success rate of 31%. ESWL performed against ureter stones showed poor results with a success rate of 63% for the stones smaller than 20 mm in diameter.

Calcium Phosphates↗
PubMed1990

[Qualitative and quantitative studies of synovial lesions in early rheumatoid arthritis demonstrated by immunocytochemistry with monoclonal antibodies].

We investigate the early stage of synovial lesions in 12 cases of rheumatoid arthritis (RA), which were diagnosed according to the criteria refined by America Rheumatism Association in 1987. The purpose of this study was to follow the inflammatory process from beginning to well known typical inflammatory features which are composed of a lot of lymphocytes and proliferated capillaries and the synovial cells. The characteristics of this study were that 1) the arthroscopy was applied for observation and getting synovial tissues, 2) 7 kinds of monoclonal antibodies were used for biopsied synovial tissues in immunohistochemistry study, further 3) qualitative and quantitative methods were adopted for identifying the infiltrating cells. These methods have developed recently in the fields of orthopaedics and immuno-histopathology, respectively. In very early stage, first, HLA/DR positive cells with large elongated cytoplasm accompanied but a few processes were found in pericapillary regions. These were neither macrophages nor lymphocytes. Following them T lymphocytes appeared and B lymphocytes appeared to aggregate in later. Quantitatively the number of HLA/DR positive cells including macrophages, B lymphocytes and a part of T lymphocytes and of Interstitial cells, increase and occupied the fairly large number of ratio in the cells of synovial tissues in RA.

Adult↗
PubMed1989

[Changes in cerebral level of monoamines by Japanese encephalitis virus infection].

The changes in monoamine levels of different brain regions following Japanese encephalitis virus (JEV) intraperitoneal inoculation were examined in experimentally JEV-infected mice. In addition, virus distribution was studied using infectivity assay and immuno-histochemistry of viral antigen. 1) The level of monoamines in brain tissues was not affected by 48 hours after viral inoculation, but marked effects were elicited at 96 hours after the inoculation. The cerebral concentration of 5-hydroxyindole-3-acetic acid (5 HIAA) was increased, while that of dopamine (DA) showed a decrease. Especially these alteration were observed in the cerebral cortex, but not in the cerebellum. 2) The viral growth in the brain was observed at 48 hours after the inoculation. The growth in the cerebellum, however, was found to be lower than those in other cerebral regions. 3) The viral antigen was detected in the cerebral cortex, hippocampus, mesencephalon and diencephalon in addition to the substantia nigra and striatum. From these results, it is presumed that clinical manifestation of JEV infection may involve the changes in the metabolism of neurotransmitter, especially those of DA and serotonin in the brain.

3,4-Dihydroxyphenylacetic Acid↗
PubMed1989

[Transurethral uretero-lithotripsy of ureteral stones in Osaka City University].

During the 39 months since the introduction of transurethral lithotripsy (TUL) for the treatment of ureteral stones at our hospital in August 1985, TUL was performed a total of 200 times in 178 patients with ureteral stones. Among them, 111 patients had left ureteral stones and 65 had right ureteral stones, while 2 patients had ureteral stones in both sides. The stones were divided into upper ureteral stone (84 patients) and lower ureteral stone (94 patients) at above and below the iliac brim. 89% of the stones were less than 2 cm in diameter. Most of the patients were given lumbar anesthesia, and a guide wire was inserted into the ureter. The ureter was dilated with a ureteral bougie, and a 13F or 14F Storz ureteroscope was inserted. The stones were disintegrated by an ultrasound lithotripto and removed using forceps and a basket catheter. After the TUL procedure, a double J catheter was indwelled and removed within 5 days. The results were evaluated by DIP which was done 2 to 3 months after TUL. The success rate included residual stones less than 4 mm in diameter, as they could be spontaneously discharged. As a result, the success rate for upper ureteral stones was 53%, and it was higher for smaller stones. On the other hand, the success rate for lower ureteral stones was 85% and significantly higher. The main reasons for failure were the upper migration of the stones (60%) and inability to insert the ureteroscope up to the stone due to ureterostenosis and ureteral perforation (39%).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

[A case of testicular tumor in an elderly man].

Testicular tumor is seen exclusively in men from 30 to 50 years old. In elderly men, the incidence of testicular tumors is rarer than that of other malignant tumors. Herein we report a case of an elderly patient with a testicular tumor comprised solely of seminoma. A 60-year-old man was seen at the urologic clinic of Osaka Kita Municipal Citizen's Hospital because of painless swelling of the left scrotal contents. At the time of clinical visit, physical examinations and scrotal sonography showed a fist-sized mass with solid echo pattern in the left scrotum. He was hospitalized for the exploration of the suspected testicular tumor. The testicular mass was removed surgically through the left inguinal incision. Histopathological examinations of the resected tumor revealed pure seminoma. Chest film, computerized axial tomography, retroperitoneal ultrasound study and specific tumor markers were all negative which indicated stage 1 testicular tumor confined to the scrotal region according to Dixon and Moore's staging. He received adjuvant irradiation at the level of mediastinal lymph node after the operation. Postoperatively, he recovered well without any evidence of recurrence. In this report, the prognosis of non-bulky pure seminoma in elderly men are briefly reviewed in association with a conventional therapy to this disease in elderly patients described in the literature.

Dysgerminoma↗