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Biomedical subjects

A Ollier

Publications and source records attributed to A Ollier.

At least 19 recordsLinked to original sources
PubMed1999

Bovine immunoglobulin G, beta-lactoglobulin, alpha-lactalbumin and serum albumin in colostrum and milk during the early post partum period.

Colostrum and milk samples from 60 Holstein-Friesian cows were analysed for concentrations and yields of immunoglobulin G (IgG), beta-lactoglobulin (beta-lg), alpha-lactalbumin (alpha-la) and serum albumin (BSA) throughout the first 16 milkings post partum (8 d of lactation) using a single radial immunodiffusion assay. Concentrations (mg/ml, means +/- SD) at first milking were IgG 59.8 +/- 28.5, beta-lg 14.3 +/- 4.6, alpha-la 2.04 +/- 0.6, BSA 1.21 +/- 0.44. Large variations were recorded for IgG concentrations (15.3-176.2 mg/ml) and yields (0.2-925 g). Cows in their first lactation produced significantly lower concentrations and yields of colostral IgG than cows in later lactations. A colostral yield of IgG below the 100 g required to prevent calf hypo-gamma-globulinaemia was found in 18.3% of the cows. The concentrations of IgG, beta-lg and BSA dropped abruptly in subsequent milkings and alpha-la concentration decreased slowly. The mean IgG concentration was < 2 mg/ml after eight milkings and < 1 mg/ml after fifteen milkings. However, IgG concentration did not differ significantly, at the 1% level, during milkings 11-15. The results were tabulated to make it possible to calculate the excess of whey proteins that would be obtained if early milks were illegally added to the milk supply.

Animals↗
PubMed1998

Anatomical reconstruction of fragments of burned human bones: a necessary means for forensic identification.

The aim of any forensic inquiry concerning accidental or criminal cremations is to identify the victims. This identification depends on an essential approach: a careful collection and ultrasonic cleaning of the fragments and splinters of burned bones, and reconstruction--as far as possible--of bone specimens, which implies that forensic anthropologists are well experienced in the field of burned bones.

Bone and Bones↗
PubMed1998

Experimental single controlled study of burned bones: contribution of scanning electron microscopy.

Burned bones were studied using Scanning Electron Microscopy. The samples were cut from a maxillary-mandibular block taken during an autopsy. These fragments were heated in a furnace under controlled temperature conditions for 60 minutes. The temperatures ranged from 150 to 1150 degrees Celsius. The results are as following: (i) there are significant alterations of the bone, more and more obvious as the temperature increases, (ii) it appears to be difficult to establish a precise correlation between the temperature and the scanning electron microscopy patterns.

Aged↗
PubMed1997

Contribution of microscopic plant anatomy to postmortem bone dating.

The authors describe a microscopical method of studying plant anatomy for estimating the age of vegetal organs which can help to date bones. This procedure was not based on counting the number of annual rings, as usually, but on demonstrating a completely primary structure without development of secondary formations; this material was considered as a part of a young root system whose development would correspond to about one year. The usefulness and limits of this procedure are discussed.

Adult↗
PubMed1997

A fully three-dimensional method for facial reconstruction based on deformable models.

Two facial models corresponding to two deceased subjects have been manually created and the two corresponding skulls have been dissected and skeletonized. These pairs of skull/ facial data have been scanned with a CT scanner, and the computed geometric three-dimensional models of both skulls and facial tissue have been built. One set of skull/facial data will be used as a reference set whereas the second set is used as ground truth for validating our method. After a semi-automatic face-skull registration, we apply an original computing global parametric transformation T that turns the reference skull into the skull to be reconstructed. This algorithm is based upon salient lines of the skull called crest lines: more precisely the crest lines of the first skull are matched to the crest lines of the second skull by an iterative closest point algorithm. Then we apply this algorithm to the reference face to obtain the "unknown" face to be reconstructed. The reliability and difficulties of this original technique are then discussed.

Cephalometry↗
PubMed1996

Identification by frontal sinus pattern in forensic anthropology.

The authors report forensic cases from the literature, as well as two personal homicide cases, of identification through comparison of frontal sinus radiographs. A general discussion about identification using frontal sinus X-rays is presented, pointing out the reliability of the method, in reference to the uniqueness of the frontal sinus in humans, but also some difficulties, especially in reference to the distance, orientation and angle of the X-ray technique.

Forensic Medicine↗
PubMed1996

Facial casting as a method to help identify severely disfigured corpses.

The authors apply a previously reported method for facial casting of severely disfigured corpses, which allowed a three-dimensional cast to be made. This method involved several stages: face restoration, casting by elastomer, then three-dimensional positive image building. This technique seems to be useful in all cases of severe disfiguration of the face, particularly by trauma.

Adult↗
PubMed1995

Method for identifying putrefied corpses by facial casting.

The authors developed an original method for casting the face of putrefied corpses, which allowed a three-dimensional facial cast of an individual to be made. This method used several stages: face restoration by subcutaneous injections of specific materials, casting by elastomer, then three-dimensional positive image building. This technique enabled the person to be recognized and then identified, and seems to be useful in such difficult cases.

Face↗
PubMed1994

An experimental methodology for the study of postmortem changes in toxic concentrations of drugs, using secobarbital as an example.

Postmortem changes in alcohol and drug concentrations are well known today. The authors used an HPLC assay of barbiturates in postmortem tissue, and developed a rat model in order to evidence postmortem changes in toxics concentrations. Postmortem changes in secobarbital concentrations were evidenced using a rat-secobarbital model. This work emphasizes the difficulty of postmortem toxicology, as concentrations found at the time of autopsy may be different from concentrations at the time of death.

Animals↗
PubMed1993

Intoxication from Veratrum album.

Two new cases of Veratrum poisoning are described. Clinical symptoms occurred quickly, within 30 min. Vomiting, a fall in blood pressure and bradycardia were observed. The outcome was favourable in both cases, producing a cure without sequellae. Examination of the literature showed that such cases are nearly always accidental, resulting from the difficulty in distinguishing Veratrum album and Gentiana.

Blood Pressure↗
PubMed1992

Milking of cows in late pregnancy: milk production during this period and during the succeeding lactation.

Fifteen lactating cows were milked throughout pregnancy, and the effects on milk performance were studied during this period and during the succeeding lactation, relative to 11 conventionally managed cows (2 months dry before calving) as controls. During the last 2 months of pregnancy, only nine cows did not dry off spontaneously. Protein and fat concentrations in milk increased rapidly, but the concentration of lactose, corrected for milk yield, did not change. The ratios of individual caseins to total protein decreased with the quantity of milk produced, but only for yields below approximately 6 kg/d. The relative proportion of kappa-casein tended to decrease in the last milkings. During the succeeding lactation (first 15 weeks after calving and first 6 weeks of grazing) continuously milked cows yielded 4 kg milk/d less than the cows of the other group. The protein content of their milk was higher (2-3 g/kg depending on the period) than that of the control group, and the lactose content tended (P less than 0.10) to be lower. Changes in the relative proportions of nitrogenous fractions with time were not different in the two groups. Differences between the two groups in the concentration of protein in milk, and in the concentration of glucose and non-esterified fatty acids in the plasma, suggest a better energy balance for the continuously milked cows during the succeeding lactation.

Animal Nutritional Physiological Phenomena↗
PubMed1992

Acute arsenic intoxication: forensic and toxicologic aspects (an observation).

The authors report on an acute suicidal arsenic intoxication (di-arsenic-trioxide). Death can occur one week after ingestion, despite intensive care. The forensic, anatomopathological and toxicologic aspects are reported. Forty titrations are realized at the level of the biologic fluid in viscera, by absorption spectrophotometry. These data are compared with those in standing literature, especially with the rates determined in normal subjects, following simple environmental impregnation.

Adult↗
PubMed1991

Duodenal rapeseed oil infusion in early and midlactation cows. 1. Intestinal apparent digestibility of fatty acids and lipids.

Rapeseed oil was infused continuously into the duodenum of lactating dairy cows. Five separate trials were conducted with differences in amount of oil infused (1.0 to 1.5 kg/d), in lactation stage (early to late), and in basal diet (corn or grass silages). The effects of lecithin addition (9 g/d) also were studied. Oil infusion did not affect the apparent digestibility of defatted organic matter. Fatty acid, ether extract, and total lipid digestibilities in control cows were 62 to 82%, 72 to 76%, and 59 to 73%, respectively. Intestinal apparent digestibilities of oil fatty acid and ether extract fractions were estimated to be 63 to 74% and 67 to 81%, respectively. Intestinal apparent digestibility of oil total lipids was only 20 to 57% due to a significant increase in nonfatty acid lipid excretion (160 to 750 g/d above control cows), which sharply decreased the energy value of the infused oil. Lecithin did not modify apparent digestibility in oil-infused cows.

Animals↗
PubMed1991

Slow release somatotropin in dairy heifers and cows fed two levels of energy concentrate. 1. Performance and body condition.

Fifty-two Holstein dairy cows (27 primiparous) were used in a 2 x 2 factorial design during wk 9 to 39 of lactation to assess the effects of slow release recombinant bST (500 mg injected at 14-d intervals) and the effects of the level of energy concentrate in the diet on dairy performance. After a winter period, cows were turned out to pasture at wk 24 of lactation. During winter, cows given low concentrate (2.5 vs. 5.4 kg DM/d) ate significantly more corn silage (13.9 vs. 11.9 kg DM/d), so that treatment had no significant effect on any measured parameter. The milk yield of bST-supplemented cows increased by 2.1 kg/d (11.0%) throughout the total experimental period. The response did not differ according to parity. Milk fat content and SCC were not altered, but milk protein was lower (.8 g/kg) from cows receiving bST during winter, and lactose increased (.5 g/kg). Lactation curves of bST-supplemented cows showed a cyclic response in milk production. Gross efficiency of milk yield was improved (.18 units) by bST administration without change in diet digestibility. No difference due to bST was found in the health or reproduction of cows. Live weight change did not differ among the groups. Multiparous cows gained body condition (bST-supplemented animals gained less than controls: .1 vs. .7 point on a five-point scale; NS). Control primiparous cows gained body condition (.1 point) but bST-supplemented cows significantly lost body condition (1.4 point).

Animal Feed↗
PubMed1990

Post mortem kinetics of secobarbital.

Post-mortem changes in barbiturate concentrations were evidenced using a rat-secobarbital model. The method used for the extraction and HPLC assay of barbiturates is suitable for all biologic fluids and post-mortem tissues. Kinetic data obtained is of excellent quality. Various modelization constants were defined. This experimental work emphasizes the difficulty of post-mortem toxicology, as concentrations found at the time of autopsy may be different from concentrations at the time of death, in blood as well as in tissues.

Animals↗
PubMed1990

[Use of urea as a marker of body water in Friesian cows].

Urea was used as a marker body water. Twelve catheterized dairy cows were injected with urea for 3 consecutive days. Equilibrium between injected and endogenous urea was obtained at 35.9 min over the 3 d. Different models to estimate urea space (EU) were compared with body water estimated at slaughter. The standard error was minimum when using the mean of EU on the 3 daily estimates obtained at 20 min. However, repeatability of EU measurement was low.

Animals↗