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A Ooshima

Publications and source records attributed to A Ooshima.

At least 127 records · Page 7Linked to original sources
PubMed1988

Immunohistochemical localization of type V collagen in normal human skin.

Tissue distribution of type V collagen in normal human skin was studied using an indirect immunofluorescent technique to determine whether type V collagen is present in the interstitium or in the basement membrane. Type V collagen was isolated from the human placenta by pepsin digestion and was purified with fractioning salt precipitations. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) demonstrated that type V collagen contained alpha 1(V) and alpha 2(V) chains, but not the alpha 3(V) chain. Specificity of the rabbit antibodies to type V collagen was assessed using enzyme-linked immunosorbent assay (ELISA) and an immunoblotting method. Antibodies showed no cross-reactivity to other collagens, laminin, and fibronectin. With an indirect immunofluorescent technique, type V collagen was found to be widely distributed throughout the dermis. Intense fluorescent staining was noted in the papillary dermis and adnexal dermis surrounding hair follicles and eccrine glands. The basement membrane of the dermoepidermal junction, skin appendages, and capillaries was not stained. By indirect immunoperoxidase double staining, type V collagen was not found to be deposited on type IV collagen present in the basement membrane. Immunoelectron microscopic studies showed that type V collagen was not located in the basal lamina. These results suggest that type V collagen is distributed in the interstitium, but not in the basement membrane of normal human skin.

Adult↗
PubMed1988

Lymph node involvement in chronic neutrophilic leukemia. An immunohistochemical study.

An immunohistochemical study was performed on autopsy material from a patient with chronic neutrophilic leukaemia (CNL) using antibodies against various cell lineage-related antigens. Proliferation of immature neutrophils with occasional clusters of erythroblasts and megakaryocytes were noted in the retroperitoneal lymph nodes, spleen, and kidneys as well as in the bone marrow. Predominance of immature neutrophils in the lymph nodes suggested the emergence of a blast crisis, although there was no increase of blasts in the peripheral blood. Since immature myeloid cells are difficult to distinguish from malignant lymphoid cells on tissue sections, we suggest that immunohistochemical identification of cell lineage-related molecules on these cells is necessary for the more accurate interpretation of lymph node lesions in myeloid neoplasms.

Bone Marrow↗
PubMed1988

Increased serum immunoreactive prolyl hydroxylase in rats with carrageenan-induced granuloma and adjuvant arthritis.

The authors developed a competitive enzyme immunoassay for serum immunoreactive prolyl hydroxylase (SIRPH) as a marker of fibrogenesis, and examined the changes in SIRPH concentrations in rats with carrageenan-induced granuloma and adjuvant arthritis. The effects of such anti-inflammatory agents as prednisolone, pranoprofen, indomethacin, and hydrocortisone were also investigated. Prolyl hydroxylase activity in rats with carrageenan-induced granuloma and adjuvant arthritis increased inflammatory granulation tissue, and the concentrations of SIRPH also increased time dependently. The nontreated controls showed a constant low level of SIRPH. After treatment with anti-inflammatory agents or removal of the granuloma, SIRPH levels decreased coincident with the improvement of clinical symptoms. It was assumed that immunoreactive prolyl hydroxylase was released into the blood stream as a result of increased turnover of the enzyme protein owing to fibrotic disorders. SIRPH could be a useful biochemical marker for assessing therapeutic effects through the actual activity of fibrogenesis.

Animals↗
PubMed1988

Immunofluorescent localization of type I and III collagens in normal human skin with polyclonal and monoclonal antibodies.

Anti-type I and type III collagen polyclonal antibodies and anti-type III collagen monoclonal antibodies were produced using type I and type III collagen extracted from the human placenta. An indirect immunofluorescence technique using these antibodies showed the same distribution of type III as well as type I collagen in the entire dermis of the normal human skin in nearly identical patterns. Previous immunofluorescent study indicated that type III collagen is present predominantly in the papillary dermis. However, our observation that monoclonal antibody recognizing the helical portion of type III collagen reacted with the entire thickness of the dermis which suggested the presence of type I and III collagen in close proximity in the whole thickness of normal human dermis.

Adolescent↗
PubMed1988

Connective tissue components in cultured parenchymal and nonparenchymal cells of rat liver. Immunohistochemical studies.

Connective tissue components were detected immunohistochemically in cultured rat hepatocytes and sinusoidal lining cells. In the Ito and endothelial cells, the staining reactions to prolyl hydroxylase were strong. The reaction in the hepatocytes, however, was weaker, and there was no staining reaction to prolyl hydroxylase in the Kupffer cells. In the Ito cells, types III and IV collagen were clearly positive, whereas reactions to type I collagen were very weak. In the endothelial cells, type IV collagen stained clearly, whereas the reaction for type I collagen was very weak, and there was no reaction for III. Laminin was clearly positive in the Ito and endothelial cells. Connective tissue components were not detectable in the Kupffer cells. The hepatocytes were positive for types I, III, and IV collagen, but the staining reactions to type IV collagen were weak. Laminin staining was almost negative. These results suggest that type IV collagen is produced mainly by nonparenchymal cells and that types I and III collagen are produced by both parenchymal and nonparenchymal liver cells.

Animals↗
PubMed1988

Collagen production in fat-storing cells after carbon tetrachloride intoxication in the rat. Immunoelectron microscopic observation of type I, type III collagens, and prolyl hydroxylase.

Monospecific antibodies directed against type I, type III collagens, and prolyl hydroxylase were used to clarify the process of liver fibrosis after CCl4 intoxication in rats by the direct immunoperoxidase method. In acute CCl4 intoxication, fat-storing cells (FSCs) were increased in number in the areas of necrosis around the central veins. These FSCs exhibited intense positive stainings for type I, type III collagens, and prolyl hydroxylase in well-developed rough endoplasmic reticula and Golgi apparatus. This was the direct evidence that the collagens formed after CCl4 intoxication are produced by FSCs. In chronic CCl4 intoxication, increased FSCs in and around the fibers also contained strong immunoreactive materials of both collagens and prolyl hydroxylase mainly in the rough endoplasmic reticula. These collagens were also present in the Golgi apparatus and vesicles close to the cytoplasmic membrane, demonstrating the exocytic process of collagen formation of FSCs. In contrast, faint immunoreactions of both collagens were found in the rough endoplasmic reticula and Golgi apparatus of hepatocytes during the process of fibrosis. These findings indicate that FSCs play an important role in fibrogenesis after acute and chronic CCl4 intoxication in the rat.

Animals↗
PubMed1988

Scar and keloidlike lesions in progeria. An electron-microscopic and immunohistochemical study.

We describe a case of progeria with an unusual skin manifestation of numerous hyperplastic scars or keloidlike nodules on the upper and lower extremities. Electron-microscopic and immunohistochemical studies indicated that the nodules consisted of various forms of collagen tissues, including type IV collagen, and that they were surrounded by la+ cells. Approximately 60% of the la+ cellular infiltrates were Leu 1+4+, OKT9+, and functioning T cells, which were often closely apposed to fibroblasts. It is suggested that progeria is associated with hyperplastic scars or keloidlike lesions with an unusual accumulation of type IV collagen, which may be formed through interaction between activated T cells and fibroblasts after minor traumas.

Biopsy↗
PubMed1987

In vitro control of neutrophilic phagocytosis of IgG-coated SRBC by macromolecules involved in released products from platelets.

Analysis of macromolecular fraction of platelet release products (PRPr) was undertaken after we observed that pretreatment of human neutrophils with macromolecules (greater than 10(5) daltons) involved in PRPr enhanced phagocytosis of IgG-sensitized sheep red blood cells (IgG-SRBC). Sephadex G-200 gel filtration of PRPr revealed two macromolecular activators and two suppressors of neutrophilic phagocytosis of IgG-SRBC: MAPP(s), macromolecular activator(s) of phagocytosis from platelets; MSPP(s) macromolecular suppressor(s) of phagocytosis from platelets. The estimated molecular weights of the respective MAPPs were 100 kdaltons to 140 kdaltons (kd) (s-MAPP) and 200 kd to 290 kd (I-MAPP) and those of the respective MSPPs were 60 kd to 100 kd (s-MSPP) and 400 kd to 500 kd (I-MSPP). It is suggested that an I-MAPP molecule has at least an s-MAPP molecule as a functional unit and that MAPPs and larger MSPP (I-MSPP) might be glycoproteins, while smaller MSPP (s-MSPP) seems to contain neither carbohydrates nor lipids. As far as the effects of serum and plasma on phagocytosis of IgG-SRBC by neutrophils are concerned, the serum gave twice as much phagocytic activity as the plasma. Substantial MAPP-like activities were observed in the serum and MSPP-like activities were observed in both the serum and plasma.

Adult↗
PubMed1987

Ultrastructural localization of type IV collagen, laminin and prolyl hydroxylase in biliary epithelial cells of rat liver following ligation of the common bile duct.

Type IV collagen and laminin are major components of basement membrane (BM), whereas prolyl hydroxylase (PH) is a key enzyme in the hydroxylation of proline to hydroxyproline in collagen synthesis. In order to elucidate the exact mechanism of the formation of BM, immune electron microscopic observation of type IV collagen, laminin and PH was made in rat liver with marked proliferation of bile ducts following ligation of the common bile duct. Extracellular localization of type IV collagen was found in the BM of bile ducts and blood vessels and in the space of Disse in both normal rat liver and the liver of rats undergoing operation. Type IV collagen was localized in lamina rara and lamina densa. Laminin was codistributed with type IV collagen in BM but rarely in the space of Disse even in the liver of rats undergoing operation. Immunostaining of laminin was diffusely spread in lamina densa, but sparsely in lamina rara. Though no reaction products of type IV collagen and laminin were detected in the cytoplasm of normal biliary epithelial cells, they were found in rough endoplasmic reticulum (rER) and the vesicles close to the basal surfaces of the plasma membrane of the proliferating biliary epithelial cells. No evident localization of these components in Golgi apparatus was found. PH was found in rER of the biliary epithelial cells, hepatocytes, endothelial cells of vessels, fibroblasts and perisinusoidal cells except for Kupffer cells in normal rat liver. More intense and diffuse staining of PH was observed in rER in the proliferating biliary epithelial cells of the liver of rats undergoing operation in concomitance with the evident localization of type IV collagen in this organelle. These findings suggest that the major components of BM, such as type IV collagen and laminin in the proliferating biliary epithelial cells, are produced in rER and secreted by vesicles to the basal extracellular spaces, thus forming new BM in these circumstances.

Animals↗
PubMed1987

Probucol prevents the progression of atherosclerosis in Watanabe heritable hyperlipidemic rabbit, an animal model for familial hypercholesterolemia.

In this study, we questioned whether in vivo probucol could prevent the progression of atherosclerosis in homozygous Watanabe heritable hyperlipidemic (WHHL) rabbits, an animal model for familial hypercholesterolemia. At 2 months of age, eight WHHL rabbits were divided into two groups. Group A (n = 4) was fed standard rabbit chow for 6 months. Group B (n = 4) was fed standard rabbit chow containing 1% probucol for 6 months. At the end of the experiments, average plasma concentrations of cholesterol were 704 +/- 121 mg/dl in group A and 584 +/- 61 mg/dl in group B, respectively. The percentage of surface area of total thoracic aorta with visible plaques in group A versus group B was 54.2% +/- 18.8% versus 7.0% +/- 6.3%, respectively. What was noteworthy was that the percentage of plaque in the descending thoracic aorta was almost negligible (0.2% +/- 0.2%) in group B rabbits compared to that in group A rabbits (41.1% +/- 20.2%). Low density lipoproteins (LDL) isolated from WHHL rabbits under treatment with probucol (group B) were shown to be highly resistant to oxidative modification by cupric ion and to be minimally recognized by macrophages. On the contrary, LDL from group A rabbits incubated with cupric ion showed a 7.4-fold increase in peroxides (thiobarbituric acid-reactive substances) and a 4.3-fold increase in the synthesis of cholesteryl ester in macrophages compared to those of LDL from group B rabbits. Thus, probucol could definitely prevent the progression of atherosclerosis in homozygous WHHL rabbits in vivo by limiting oxidative LDL modification and foam cell transformation of macrophages.

Animals↗
PubMed1986

A sandwich immunoassay for human prolyl 4-hydroxylase using monoclonal antibody.

Monoclonal antibody was used in a sandwich enzyme immunoassay and in a radioimmunoassay for human serum immunoreactive prolyl 4-hydroxylase. The enzyme immunoassay utilized a monoclonal antibody as a solid phase and horseradish peroxidase-labeled rabbit antibody (Fab') to human prolyl 4-hydroxylase as a conjugate. Sensitivity was 0.1 ng (0.4 fmol) of enzyme per tube. With a conjugate purified by an enzyme-bound affinity column, sensitivity was increased to 0.01 ng (0.04 fmol) per tube, and linearity was obtained between 0.01 to 30 ng (0.04-125 fmol) per tube. The radioimmunoassay used a 125I-labeled rabbit antibody (IgG) as the conjugate. Sensitivity of this technique was 0.4 ng of enzyme per tube. The enzyme immunoassay gave reproducible quantitation and evidenced a higher enzyme concentration in the serum of patients with liver disorders. Protein immunoblotting showed that the serum immunoreactive prolyl 4-hydroxylase trapped in the sandwich immunoassay was mainly the beta-subunit.

Antibodies, Monoclonal↗
PubMed1986

Immunolocalization of type III collagen and procollagen in cirrhotic human liver using monoclonal antibodies.

Immunolocalization of type III collagen and procollagen in cirrhotic human liver was studied using monoclonal antibody specific for the helical determinant of type III collagen extracted from human placenta. Deparaffinized, trypsin-treated cirrhotic liver sections from 8 autopsy cases were examined by the unlabeled peroxidase-antiperoxidase and immunofluorescence techniques. These techniques revealed the localization of this epitope shared by type III collagen and procollagen not only in the extracellular matrix of hepatocytes and sinusoidal cells but also in the cytoplasm. In hepatocellular carcinoma concurrent with cirrhosis, neoplastic cells were shown to react with this antibody as well. These results are consistent with data obtained using antiserum specific for bovine type III procollagen aminopeptide which appeared in our previous report.

Aged↗
PubMed1986

Primary hepatocellular carcinoma with hepatitis B virus-DNA integration in a 4-year-old boy.

The autopsy findings in a case of hepatocellular carcinoma with hepatitis B virus (HBV)-DNA integration in a Japanese boy, 4 years and 10 month of age, are reported. The boy was an HBV carrier born in a typical familial cluster of HBV infection in Japan. He had been asymptomatic until the sudden manifestation of the liver tumor. Histopathologic examination revealed a well-differentiated, adult-type hepatocellular carcinoma without hepatic cirrhosis. HBV-DNA sequences were detected in the tumor cell DNA by the Southern blot hybridization method. This is the youngest patient with hepatocellular carcinoma with HBV-DNA integration reported to date, suggesting that HBV may manifest its oncogenic properties after a shorter incubation period than generally believed.

Antigens, Viral↗
PubMed1986

Immunological properties of monoclonal antibodies to human and rat prolyl 4-hydroxylase.

Monoclonal antibodies to human (8 clones) and rat (12 clones) prolyl 4-hydroxylase [EC 1.14.11.2] were prepared and characterized as regards subclass, subunit specificity, inhibition and crossreactivity. Among the antibodies to the human enzyme, four clones showed the IgG1 subclass, two IgA, one IgG2b, and one IgM. Four clones reacted with the alpha subunit of the enzyme, while the others reacted with the beta subunit. The enzymatic activity was inhibited by four clones. Five clones crossreacted with the rat enzyme. One clone inhibited the rat enzyme. Among the antibodies to the rat enzyme, seven clones showed the IgG1 subclass, four IgG2a and one IgG2b. Seven clones reacted with the alpha subunit, and four with the beta subunit. One reacted with neither subunit. The enzymatic activity was inhibited by seven clones. Seven clones crossreacted with the human enzyme. Three clones inhibited the human enzyme.

Animals↗
PubMed1985

Activation of neutrophil phagocytosis of complement coated and IgG coated sheep erythrocytes by platelet release products.

Phagocytosis by human neutrophils of opsonized sheep erythrocytes with rabbit immunoglobulin M and human complement (IgM-EACh) and those with rabbit immunoglobulin G (IgG-EA) increased 2-3 times of control neutrophils after treatment with released products from activated platelets (PRPr). The factors in PRPr which augmented IgM-EACh phagocytosis were suggested to be adenosine diphosphate (ADP) and triphosphate (ATP) by experiments using apyrase treated PRPr and by direct exposures of neutrophils with these nucleotides. The presence of two other stimulators for phagocytosis of IgG-EA were suggested. One was a macromolecular substance of molecular weight greater than 100 000 and the other was a substance of molecular weight less than 500. The latter may be a prostaglandin because the activity was easily removed by acid-extraction with ethylacetate from the water phase, and because prior incubation of platelets with 10 micrograms per ml indomethacin deprived PRPr of its low molecular weight activity. Exposure of neutrophils to thromboxane B2, prostaglandin F2 alpha and prostaglandin E2 at the concentration of 10(-6)-10(-7) mg/ml actually increased the phagocytosis of IgG-EA. The activity in the macromolecular fraction was not affected by prior incubation of platelets with indomethacin.

Adenosine Diphosphate↗
PubMed1981

Collagen alpha B chain: increased proportion in human atherosclerosis.

In a study of human atherosclerotic plaques, the relative abundance of alpha chains in pepsin-solubilized collagens from 28 human aortas was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ratio of alpha B, a component of the alpha chain in type V collagen, to alpha 1(I) was markedly increased in the atherosclerotic plaques compared to the nonsclerotic intact media and adventitia. It is suggested that proliferating smooth muscle cells in the sclerotic lesion were transformed to synthesize a larger amount of collagen alpha B chain during the process of human atherogenesis.

Aged↗