Biomedical subjects
Publications and source records attributed to A Orozco.
A C1q solid phase microassay was designed for the rapid detection of circulating immune complexes. Its level of sensitivity is comparable to that of the Raji cell and greater than the C1q binding assay; furthermore, it is faster and low in cost. These conditions make it more practical and applicable in the clinical setting.
Circulating immune complexes (CIC) have been investigated in 100 normal subjects; the RIA-Raji and the C1q-BA conventional methods, as well as a new solid phase microassay utilizing purified C1q and the systematic search of cryoprecipitates were employed. CIC serum levels did not differ in regards to sex; in relation to age, values for C1q-BA were identical in subjects from 0 to 60 years and also in those beyond age 60; the differences encountered by RIA-Raji or by the C1q-SP microassay in these two main groups were not statistically significant. Cryoprecipitates were present in 100% of the 68 examined subjects. Immunoglobulins (G, A and M), anti-nucleic acid (DNA and Poly A) and CIC (by the three methods) were present in the cryoprecipitates while lymphocytotoxins, rheumatoid factor and C3 were undetectable; protein content of the cryoprecipitates increased significantly with age, reaching a normal superior limit of 0.52 mg/ml beyond age 30. These findings further support the role played by CIC in normal immune response and may help in the understanding of the physiopathology of clinical conditions associated with immune complexes.
A new simple method, which allows rapid access to the ventricular system to monitor intracranial pressure, is described. One of the lateral ventricles is punctured percutaneously, with a hand drill operated by a flash-light battery, and a lumbar puncture needle (20 gauge) attached to a water manometer or to a strain gauge connected to a venous pressure monitor.
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Although abundant, available information regarding the values of circulating thyronines in the rainbow trout is misleading and confusing. The present study demonstrates the importance of maintaining species serum-homology when measuring thyronines with RIA to avoid spurious results. The utilization of homologous (trout) serum free of thyronines in the standard curve allowed the standardization of accurate and specific radioimmunoassays to quantitate circulating T3 and T4 levels in 10 microliters of rainbow trout serum. Detection limit was 50 pg for both thyronines. Inter- and intra-assay coefficients of variation were minor to 10%. Mean values for T3 and T4 +/- SEM in the trout population studied (n = 34) were 23.6 +/- 1.2 and 12.0 +/- 2.3 ng/ml respectively. A positive and significant correlation (p < 0.005) between body weight and T3 was observed, confirming the active participation of this thyronine in growth processes.
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