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Biomedical subjects

A Orts

Publications and source records attributed to A Orts.

30 records · Page 2Linked to original sources
PubMed1977

Effect of fosfomycin on the thrombocytal aggregation induced by ADP.

The effect of fosfomycin on the thrombocytal aggregation induced by ADP was studied. Plasma rich in thrombocytes was used. It was obtained from blood donors. Fosfomycin was used in concentrations of 1.3 X 10(-3); 1.3 X 10(-2), and 1.3 X 10(-1)M and the ADP in concentrations of 1.17, 2.34 and 3.51 X 10(-6)M. Two experimental groups were used: the first group was made up of five experiments, with an incubation time for the fosfomycin with the thrombocytes of 2 min; the second experimental group was made up of five experiments whose incubation times were 5 and 10 min. Fosfomycin inhibits the thrombocytal aggregation which is induced by ADP. This inhibition (1) is larger, the smaller the concentration of ADP, (2) for a single concentration of ADP it is proportional to the concentration of fosfomycin that is used, and (3) there are no significant differences according to the time of incubation. This thrombocytal anti-aggregating activity is related to the presence of a phosphonic group in the molecule of fosfomycin.

Adenosine Diphosphate↗
PubMed1976

[Modifications induced through beta receptors in adrenergic nervous system and gastric ulcers (author's transl)].

The experimental relation between adrenergic nervous system and ulcus has been studied. Administration of adrenaline to rats did not induce any ulcers, or showed any noteworthy modification in the studied pH, acidity and peptic activity of gastric content parameters. The number of ulcers induced by the Shay method, acidity and peptic activity decreased after beta receptor blockage. Ko-592 did not induce any significant change on these parameters when the animals were subjected to stress conditions by restraint. Beta receptors discretely participate in ulcer pathology.

Animals↗
PubMed1976

[The effect of oxytocin and dihydrostilbestrol on various uterine cations (author's transl)].

The effect of dihydrostilbestrol diacetate and oxytocin on the Na+, K+, Ca++ and Mg++ content in uterine Wistar rats has been studied. A bilateral ovariectomy on the animals was performed. After seven days dihydrostibestrol (0.25 mg/kg a day) and oxytocine (0.025 U.I./kg a day) were administered separately and simultaneously i.p. during a period of five days. Twenty four hours after the first, third and fifth administration, five rats from each group were decapited and the above uterine cation content was determined by atomic absorption spectrometry. Dihydrostibestrol induced a marked increase and a slight decrease in K+ and Na+ contents respectively, lowering the Na/K ration. It also increased greatly Ca++ and Mg++ content. Oxytocin induced a Na+ and K+ increase, a Ca++ decrease, and slight changes in Mg++. These results partially confirmed the bioelectric and biomechanic alterations produced by the above hormones on the uterine cells.

Animals↗
PubMedDate not supplied

Effects of hypoxia on retinal pigmented epithelium cells: protection by antioxidants.

Age-related macular degeneration and other eye diseases, such as diabetic retinopathy, are probably linked to the effects of oxygen radicals derived from light or metabolic reactions. We have investigated the effects of hypoxia on bovine retinal pigmented epithelial cells (RPE) and the response of these cells to two antioxidants that have previously shown a beneficial action against free radical-linked senescent involution. The main results of the study were as follows: (i) Hypoxia induced apoptotic damage on RPE cells, with LDH leakage and ATP reduction; (ii) both vitamin C (VC) and N-acetyl-cysteine (NAC) treatment protected against hypoxia-induced apoptosis, with less DNA fragmentation. In our opinion, these findings justify further experimental and clinical work to investigate the role of hypoxia in the mechanisms of age-related RPE injury and death as well as the potential of antioxidant administration to prevent or delay retinal degenerative processes caused by oxygen-dependent pathophysiological conditions.

Acetylcysteine↗
PubMedDate not supplied

[Culture media for the detection and the identification of Streptococcus agalactiae].

Streptococcus agalactiae, a Group B streptococcus, is the main cause of bacterial perinatal infection and is also an important opportunistic pathogen. Detection and identification of S. agalactiae are straight forward with special culture media, where Group B streptococci show a specific, typical pink or red pigment. To quickly and easily detect the pigment, culture media should contain: (i) starch; (ii) an inhibitor of the folate pathway; (iii) animal serum; (iv) a pepsic proteic hydrolysate; and (v) glucose, together with a high-capacity buffer. When selective antibiotics are added to culture media designed in this way, it is possible to detect S. agalactiae directly from clinical samples by observation of its pigment after less than 12 hours of aerobic incubation.

Culture Media↗