Biomedical subjects

A Osornio-Vargas

Publications and source records attributed to A Osornio-Vargas.

5 recordsLinked to original sources

PubMed1998

Human cervical cancer-associated nuclear matrix proteins.

The nuclear matrix is the nonchromatin protein structural component of the nucleus that governs nuclear shape and also exerts regulatory control over higher order gene organization. Recent studies have documented the presence of tumor-associated nuclear matrix proteins in several human cancers. We used high-resolution two-dimensional gel electrophoresis to compare nuclear matrix protein patterns in cervical carcinomas with those from normal cervical tissue. Tumors obtained from 20 patients undergoing hysterectomy for clinically localized cervical cancer were compared with normal cervical tissue. We have identified five polypeptides (CvC-1: Mr = 69,408 Da, pI = 5. 78; CvC-2: Mr = 53,752 Da, pI = 5.54; CvC-3: Mr = 47,887 Da, pI = 5. 60; CvC-4: Mr = 46,006 Da, pI = 5.07; and CvC-5: Mr = 44,864 Da, pI = 6.61) in the nuclear matrix from cervical carcinomas that were present in 20 of 20 cervical tumors but 0 of 10 normal tissues. These data extend similar findings of cancer-associated nuclear matrix proteins in other human cancers and suggest that nuclear matrix proteins may represent a new class of cancer markers that could aid the diagnosis or management of some types of cancer.

Antigens, Nuclear↗

PubMed1994

Application of an autopsy report specifically designed for cardiovascular diseases.

After modifying the format and content of the autopsy report traditionally used at the Instituto Nacional de Cardiología of México, Tlalpan, we present the results of the new, revised autopsy protocol here. The new autopsy protocol (model report) was designed as a "fixed format" to describe the cardiovascular diseases observed at our institution, thus pretending to collect more and better data, providing useful information, and contributing to better clinicopathologic correlations. The comparison between the model and traditional reports demonstrated an improvement in the autopsy data collection system--achieving better clinicopathologic correlations of the main disease and the cause of death, and identifying morphologic alterations that would explain it in more cases. The model report was also considered more accessible and useful since it permitted the answering of more questions raised by clinicians.

Autopsy↗

PubMed1993

Modification of the Smith and Naylor technique for the identification of ferruginous bodies.

Smith and Naylor's technique has been successfully used by many authors around the world to recover inorganic particles from the lungs. In this short report we compare the results of counting inorganic particles recovered from human lung tissue by two different methods: Smith and Naylor's technique and a modification to it. We used ferruginous bodies (FB) as markers for inorganic particles and we compared the results of the FB counts in both methods. In the traditional technique the interface formed in the 1:1 ethanol-chloroform mixture is discharged and FB are counted only in the formed pellet. Our modification also quantified FB in the interface fraction. Post-mortem samples from each of the lung lobes were taken from 22 individuals, totalling 198 samples. Each sample was digested in liquid commercial bleach and processed by both techniques. Our results showed that the modified technique was more sensitive in the detection of FB than the traditional method, with 16 out of 22 individuals showing positive identification against 14 out of 22, respectively. Furthermore, the modified technique proved not only to be more sensitive, but also almost twice as accurate: 49 FB/g vs. 26.7 FB/g of dry tissue.

Evaluation Studies as Topic↗

PubMed1985

Extrapulmonary silicosis: a clinical, morphologic, and ultrastructural study.

A variety of silicotic lesions derived from thoracic silicosis via lymphohematogenous spread to the liver, spleen, bone marrow, and extrathoracic lymph nodes are described. The morphologic features of these lesions depend on the extent of macrophage aggregation, the occurrence of fibrogenesis, and the development of necrosis and degradative changes in macrophages and adjacent extracellular matrix, presumably caused by lysosomal enzymes released from macrophages. Ultrastructurally, the degenerative alterations of matrix material include longitudinal splitting and breakage of collagen fibrils into segments one and three quarters the length of the original fibrils and deposition of flocculent electron-dense material either focally or diffusely around collagen fibrils. The corresponding changes viewed light microscopically are those of fibrinoid necrosis. The sclerohyaline nodule, the characteristic lesion of silicosis, includes all of these features as it evolves through nodular histiocytic and subsequent fibrohistiocytic phases. Its ultimate morphology appears to be determined by the reassembly of the degraded matrix into non-native, fibrous long-spacing collagen via a spiny collagen intermediary. The sclerohyaline nodule occurs infrequently in the spleen and liver, although less typical lesions caused by silica alone or admixed with other dusts seem to occur more commonly in these organs. These lesions appeared as loose or nodular histiocytic or fibrohistiocytic aggregates. Nonspecific fibrous nodules or more extensive fibrosis, as seen in portal triads, may represent advanced stages of such lesions. Acute or healed focal segmental glomerulonephritis occurred in 40 per cent of the cases, suggesting that it may be an important remote effect of silicosis. Continuous destruction of lymphocytes adjacent to silicotic nodules may be an antigenic source of the high concentration of autoimmune reactants described in silicosis.

Adult↗

PubMedDate not supplied

[Experimental reevaluation of myocardial ondulations in the early histological diagnosis of myocardial infarct].

This investigation was undertaken in order to experimentally reassess the value of myocardial waviness and stretching as early histological indicators of acute myocardial infarction. Twenty three dogs were subjected to periods of ischemia, from 30 minutes to 4 hours; wavy fibers were present in 87% and 91% of the ischemic and non-ischemic samples respectively. It is concluded that myocardial fiber waviness lacks significance as an indicator or early myocardial infarction, whose diagnosis remains a major challenge.

Animals↗