Biomedical subjects
Publications and source records attributed to A P Sadkov.
Methods of biochemical and physicochemical analysis were applied for studying the role of Micrococcus luteus cell structure in gold accumulation. It was shown that membrane proteins were the main factors in this process. Moreover, different quinones played the main role in the redox transformation of gold.
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The electron microscopy method has been used for studying the topography of the active centre of the nitrogenase in combination with the method of electron density labels. The active centre has been shown to place on the nitrogenase macromolecule asymmetrically. The non-heme iron atoms of the nitrogenase active centre have been regularly packed and located on the macromolecule as a quadrangular cluster closely to the surface of subunits. Two free sulfhydryl groups of the ATP-site of the nitrogenase active centre are in close proximity to the cluster. ATP-site of the nitrogenase active centre containing these groups is located on the Fe-enzyme, while the main part of the iron-containing cluster is located on the Mo-Fe-enzyme.
The isotopic effect during oxidation of methane and deuteromethane by a suspension of Methylomonas rubrum cells, for which methane is the only source of carbon, was observed. The rate of CH4 oxidation is 12.5 times higher than that of CH4 oxidation. It is demonstrated that CD4 is a competitive inhibitor of CH4 oxidation. The results obtained suggest that the disruption of the C-H bond is the limiting step of enzymatic oxidation of methane.
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A method of statistical processing of electron micrographs of molecular objects modified by electron-dense labels containing mercury is proposed. The method allows one to study size, degree of modification and heterogeneity of objects. Application of the method for study of modified nitrogenase and its Fe-Mo containing co-factor, lysozyme, myoglobin, sodium thiomolybdate and trichlortriazine has shown the features of chemical modification and electron micrographs of these molecules. The method can be used for processing of data about complex biological objects modified by electron-dense labels.