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Biomedical subjects

A Pagès

Publications and source records attributed to A Pagès.

At least 19 recordsLinked to original sources
PubMed1998

Rat messenger RNA for the retinal pigment epithelium-specific protein RPE65 gradually accumulates in two weeks from late embryonic days.

The RPE65 protein appears late during the retinal development. To study the basis for this regulation, the rat RPE65 cDNA was sequenced and the mRNA subsequently quantitated at various stages by competitive RT-PCR. RPE65 mRNA was detected as early as E18 (36 copies/ng of whole eye total RNA). It gradually accumulates up to P12 (27000 copies/ng) at which point it reaches a steady state level. This increase is interrupted for 3 days (P2-P4) during which the levels of mRNA remain stable. This timing and rate of accumulation parallels that of rat and mouse opsin mRNA and suggests that common factors may control the activation of genes in photoreceptors and retinal pigment epithelium cells.

Animals↗
PubMed1997

Turkey rhinotracheitis virus and Escherichia coli experimental infection in chickens: histopathological, immunocytochemical and microbiological study.

The aim of this study was to evaluate the response of chickens to a combined infection with turkey rhinotracheitis virus (TRTV) and Escherichia coli O78:K80. Groups of specific-pathogen-free chickens were inoculated by eyedrop and intranasal routes with TRTV and/or E. coli O78:K80. Presence of E. coli O78:K80, histopathological changes and tissue distribution of viral antigen in the respiratory tract of chickens were evaluated. Dual infection resulted in increased severity of clinical signs, and macroscopic and microscopic lesions compared with those groups given single infections. All 36 chickens inoculated with TRTV plus E. coli O78:K80 showed severe rhinitis. Moreover, periorbital edema and fibrinous airsacculitis and pericarditis were observed in one of the three chickens inoculated with both agents and sacrificed at day 5 p.i. In addition, purulent material in the air spaces of the cranial bones was seen in three of the six animals from the same group sacrificed at days 5 and 7 p.i. The distribution of viral antigen in tissues was similar in groups inoculated with TRTV and TRTV plus E. coli, but viral antigen was detected only in main bronchi of chickens from the latter group. The quantity of E. coli O78:K80 isolated from the nasal cavity was greater in the group given dual infection. The results obtained suggest that TRTV may act as primary agent, enhancing E. coli multiplication. The lesions observed in the group inoculated with both agents could correspond to an initial stage of swollen head syndrome (SHS) and contribute to the hypothesis that SHS could be due to a mixed infection with TRTV and E. coli.

Animals↗
PubMed1996

Ultrastructural study of turkey rhinotracheitis virus infection in turbinates of experimentally infected chickens.

Ultrastructural changes associated with turkey rhinotracheitis virus infection were studied in turbinates of chickens experimentally infected with the isolate CVL 14/86/1. Chickens were sacrificed at 3, 5 and 7 days after inoculation and samples of the middle turbinate were taken, fixed, dehydrated and embedded in an hydrophilic resin. An immunofluorescence technique on semithin sections was carried out and viral antigen was observed in the cytoplasm and associated to cilia of the turbinate epithelial cells, on days 3 and 5 after inoculation. Ultrastructurally, gold stained intracytoplasmic nucleocapsid aggregates of turkey rhinotracheitis virus were observed in ciliated and non-ciliated epithelial cells, as well as budding virus particles, at days 3 and 5 postinoculation. Different ultrastructural abnormalities, including cytoplasmic blebs, clumping and loss of cilia were observed in the apical cell membrane of many infected cells, associated with the presence of intracytoplasmic inclusions. On day 5 after inoculation, substitution of ciliated and non-ciliated epithelial cells was noted and many desquamated epithelial cells were observed within the lumina. Regenerative changes in the ciliated epithelium were observed by day 7 postinoculation. These results indicate that turkey rhinotracheitis virus is able to replicate in ciliated and non-ciliated epithelial cells causing severe alterations to the cell surface and ciliary apparatus of the turbinate epithelium. Viral-induced damage to the turbinate epithelium could enhance the susceptibility of epithelial cells to secondary bacterial infection.

Animals↗
PubMed1994

[Congenital cystic adenomatoid malformation of the lung. Apropos of 7 cases].

Seven new cases of congenital cystic adenomatoid malformation of the lung have been observed in our institution during the past twelve years. The diagnosis was made in five cases on stillborn fetuses between 16 to 23 weeks of gestation and in two cases in a three-day-old infant and in a two-month-old baby. Adopting the histological classification proposed by Stocker, the seven cases observed were type II. A review of the literature on this malformation reveals the most important characteristics of this entity (clinical aspects, macroscopic and microscopic features, associated malformations) and provides certain elements of the pathogenesis which remains unclear. Finally, only histological examination can confirm the diagnosis of this malformation.

Cystic Adenomatoid Malformation of Lung, Congenita↗
PubMed1993

[Primary lymphoma of the central nervous system. Multiple remission with corticosteroid therapy].

A primary T-cell lymphoma of the central nervous system was diagnosed at autopsy in a 73-year-old woman. The course of the illness was made of multiple neurological episodes: hypersomnia, palsies of cranial nerves, aphasia, hemiparesis, epileptic fits during 4 years, with fast recoveries under corticosteroid therapy. CT scan and MRI abnormalities were also dramatically improved by the treatment.

Adrenal Cortex Hormones↗
PubMed1993

[Cervical thymic choristoma ].

Neck and thyroid tumors probably of thymic origin are more frequently reported since two years. It is therefore of interest to specify incidence and way of production of cervical ectopic tissue from they issue. So, authors studied 763 autopsies of fetus of various ages, newborns and infants and surgical pieces of 8 children and adults. Analysis of 72 cases of cervical thymic chorista.

Adolescent↗
PubMed1992

[Malignant mixed mesodermal tumor of the ovary. A review of the literature apropos of a case with immunohistochemical study].

A very large left ovarian tumor in a 76 year old woman, on microscopic study, consisted of an epithelial component: both glandular and squamous, and a sarcomatous component: both cartilage and striated muscle. Epithelial and sarcomatous patterns were intermingled in a spindle-cell stroma. Immunohistochemical study revealed, in these spindle cells, cytokeratin-positive anaplastic epithelial structures and vimentin-positive mesenchymal structures. A review of the literature concerning this rare ovarian tumor especially including cases with a immunohistochemical study is added. However, immunohistochemical study does not allow histogenetic interpretation.

Aged↗
PubMed1991

[Thalamo-peduncular germinoma].

A 24 year-old patient presented with chronic intracranial hypertension, palsy of both abducens nerves and upward gaze, and right hypoesthesia. CT scan and MRI showed a left thalamic tumor infiltrating the left cerebral peduncle. A stereotactic biopsy revealed a germ cell tumor. Following radiotherapy the patient recovered except for ophthalmoplegia. The tumor totally disappeared on CT scan and MRI.

Adult↗
PubMed1986

Radiation-induced intracranial fibrochondrosarcoma.

A 50-year-old patient developed a left temporal fibrochondrosarcoma 27 years after radiation therapy for a suspected pituitary adenoma. The long latency between the irradiation and the development of the sarcoma and the histological nature of the tumour are unusual features for a malignant radiation-induced intracranial tumour. The histogenesis of fibrochondrosarcoma of the brain is discussed.

Adenoma↗