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A Pich

Publications and source records attributed to A Pich.

At least 73 records · Page 4Linked to original sources
PubMed1991

Papillary eccrine adenoma. A histopathological and immunohistochemical study.

A case of papillary eccrine adenoma of the thigh of a 63-year-old woman is reported. Histologically, the tumor was composed of cystic dilated and branching tubular structures with papillary projections into the lumen, surrounded by a fibrous stroma. In addition, in some areas it was composed almost exclusively of solid strands of epithelial cells. The tumoral eccrine differentiation was assessed by immunohistochemical studies. Problems of differential diagnosis with low-grade sweat gland carcinoma and with tubular apocrine adenoma are discussed.

Cystadenoma↗
PubMed1988

Angiogenic activity of hyperplastic and neoplastic lymph nodes.

Angiogenic capacity was tested in 14 non-Hodgkin's lymphomas, 7 Hodgkin's lymphomas and 15 cervical lymph nodes nonneoplastic but draining a territory with a laryngeal carcinoma. The objective was to find out whether different groups of lymphomas showed differences in their angiogenic capacity and to compare the ability to induce neovascularization of neoplastic lymphocytes. Frequency and intensity of the angiogenic response were similar for classes of lymphomas different for morphologic and immunologic characteristics. The presence of a carcinoma was sufficient to induce in tributary, nonmetastatic lymph nodes an angiogenic activity comparable to that known to characterize antigenically stimulated lymphocytes.

Animals↗
PubMed1987

Immunohistochemical identification of the papillomavirus in uterine cervix: relationship with dysplasia, koilocytosis and evolution of the lesions.

Papillomavirus common internal capsid antigen (PV-Ag) was demonstrated using an immunoperoxidase technique in 74 out of 239 (31.0%) cases of cervical condylomata diagnosed histologically. All the positive cases but 2 (72/74) had nuclear changes compatible with dysplasia or cervical intraepithelial neoplasia (CIN). Immunocytochemically determined PV-Ag positivity was found in 54 out of 92 CIN I (58.7%), in 14 out of 32 CIN II (43.8%) and in 4 out of 18 CIN III (22.2%). The extent of koilocytosis in the epithelium had a direct correlation with the PV-Ag positivity: when koilocytosis extended to more than two thirds of the epithelium thickness, 24/41 cases (63.4%) were positive; when between one third and two thirds, 32/105 cases (30.5%) were positive; when present in less than one third 16/93 cases (17.2%) were positive. The positivity was 41.8% under 30 years of age and 20.5% over 30. Out of 16 positive cases followed with multiple biopsies for 6-20 months, 9 remained positive without change in CIN degree and 7 became negative, with CIN regression in 4 cases and progression to CIN III with disappearance of koilocytosis in 3 cases. More specific methods than histocytopathology alone are needed for epidemiologic and follow-up studies, especially in the cases in which the disappearance of antigenic and morphological expression of PV infection is not followed by dysplasia regression.

Adult↗
PubMed1985

Lymphoid nodules and nodular lymphoid hyperplasia in bone marrow biopsies.

Out of 2,474 bone marrow biopsies we have observed 330 cases (13.3%) with presence of lymphoid nodules (LN). LN were frequent in old age (24.6% over 80 years), in females (17%) and in some diseases, such as rheumatoid arthritis and systemic lupus erythematosus (73.7% of the cases), partial aplasia (34%), hypersplenism (30.4%), hemopoietic dysplasia (25%), chronic renal failure (20.4%), polycythemia vera (20.2%), idiopathic thrombocytopenic purpura (18.8%), acute leukemia (17.7%). Nodular lymphoid hyperplasia of the bone marrow was found especially in systemic autoimmune diseases (26.3%), hypersplenism (9.8%), preleukemia (7.3%) and acute leukemia (4.2%). The presence of excessive medullary LN could indicate a bone marrow microenvironment damage, possibly of autoimmune origin.

Adolescent↗
PubMed1984

[Retrosternal thyroid cancers. Considerations on 25 cases].

Intrathoracic thyroid cancer is an unusual finding, and few reports are available on this item. Literature data have been reviewed, and some considerations on epidemiologic characteristics of the disease and on its treatment were gained from an analysis of reports based on the files of I Surgical Clinic of the University of Turin, Surgical service of the Oncological Department of the S. Giovanni Battista Hospital and II Pathological Anatomy Service of the University of Turin. No correlation was found between previous thyroid disease, sex, histological findings, previous radiotherapy and incidence of the disease. Older age at the onset and recurrent laryngeal nerve lesions are frequently associated with intrathoracic thyroid cancer; papillary carcinoma is rarely found. Intrathoracic situation is not likely, in Author's opinion, to affect the long term prognosis more than usual risk factors.

Adult↗
PubMed1977

Production of casein and the presence of estrogen receptors in human breast cancer.

The relationship between the presence of estrogen receptors and casein (evaluated on a semiquantitative basis with a specific immunofluorescence method) was statistically analyzed in 50 cases of human breast carcinomas. No significant correlation was found between these two parameters, whereas a relationship was established between the production of casein and the degree of histological differentiation. The results of this study, like those of other studies, revealed a lack of correlation between the presence of estrogen receptors and the degree of histologic differentiation.

Breast Neoplasms↗
PubMed1977

Morpho-functional differentiation in lobular carcinoma of the breast.

Lobular carcinoma of the breast has been studied using histochemical methods for mucosubstances; immunocytochemical methods for casein and actin; the ruthenium red electronycytochemical method for acid glycoproteins and an immunoelectroncytochemical method for casein. Mucosubstances and casein showed a similar cytoplasmic localization, but casein production was much more intense and also showed a more diffuse cytoplasmic localization. Occasionally casein assumed the form of target-like 'inclusions' as seen characteristically with the mucosubstances. The neoplastic cells were not stained by antisera against actin. Ultrastructurally, some cells showed an intracytoplasmic lumen with microvilli and/or an irregular outline at one extremity which was covered by microvilli. An electron-dense 'fuzz' and casein coated the microvilli of cells exposed respectively to ruthenium red and an anticasein serum followed by peroxidase--anti-peroxidase complexes. It is concluded that lobular carcinoma shows evidence of epithelial rather than myoepithelial differentiation with the emphasis on epithelial secretory cells engaged in intensive milk protein production. All 10 tumours tested for oestrogen receptors were positive in contradistinction to ductal carcinoma with a lower incidence of positivity. It appears that, in addition to distinctive histological and histochemical features, lobular carcinoma has an almost constant endocrine pattern in respect of its oestrogen receptor content.

Actins↗
PubMed1976

Immunocytochemical detection of casein and casein-like proteins in human tissues.

By means of specific immunochemical methods, material reacting with anti-human casein antisera has been detected in various human tissues and mainly in ductules of the breast, in the sebaceous and sweat glands of the skin, in the bronchial epithelium and glands, at the surface of some alveoli of the lung, in the exocrine pancreas, in the glands of the endometrium in proliferative phase and in the distal and collecting tubules of the kidney. The exact chemical nature and the physiological significance of the proteins present in extramammary sites and reacting with anti-casein antibodies are not clear; it appears, however, that casein might be a protein not specific of the breast, and that casein-like material might be present in other areas and mainly devoted to exocrine secretion. The interest of these findings is also related to the presence of proteins reacting with anti-human casein antisera in carcinomas of the lung, the endometrium and the gastrointestinal tract.

Breast↗
PubMed1975

Immunofluorescence detection of casein in human mammary dysplastic and neoplastic tissues.

To detect and localize casein in human mammary dysplastic and neoplastic tissues, an indirect immunofluorescence method has been devised. Anti-casein antibodies have been obtained from rabbits immunized with casein isolated from human milk. Cryostat sections post-fixed in alcohol and paraffin sections from routinely formalin-fixed tissues proved suitable. The immuno-fluorescence method revealed the presence of casein in epithelial cells in mammary dysplasia and in some, but not all, cases of carcinoma of the breast. Well differentiated (Grade I) carcinomas were shown to contain casein, mainly localized at the inner border of the epithelial cells; the milk protein was present also in cells metastatic in lymph nodes. In Paget's disease of the nipple, casein could be detected in neoplastic cells in the ducts and also in cells identifiable with the typical intra-epidermal Paget cells. This finding is evidence of a functional differentiation of Paget cells along lines proper to the mammary epithelium.

Animals↗
PubMed1975

Mammary and extramammary Paget's disease. An immunocytochemical study.

The localization and distribution of human casein has been investigated in 20 patients with Paget's disease (16 with the mammary and 4 with the extramammary form) by means of immunofluorescence and immunoperoxidase techniques. This milk protein has been detected in neoplastic cells in intraductal carcinomas of the nipple and in intraepidermal cells identifiable as Paget cells. The degree of the staining varied in different cells of the same case and in different cases. Some casein-containing intraepidermal cells, as revealed by immunofluorescence, could not be recognized after retaining of the sections as Paget cells: they could not morphologically be distinguished from other basally located epidermal cells. This finding raises the question of the existence of "pre-Paget" cells. The results obtained are discussed in relation to theories on the origin and nature of Paget cells. The immunocytochemical methods for casein detection might also be find possible application in the diagnosis of Paget's disease.

Antigens↗
PubMedDate not supplied

Correlation between argyrophilic nucleolar organizer region counts and labelling index in multiple myeloma.

The correlation between the bromodeoxiuridine (BrdU)-labelling index (LI) of plasma cells and a new proliferation marker, the Argyrophilic Nucleolar Organizer Regions (AgNORs), was investigated in 44 myeloma patients at diagnosis. A preliminary analysis was made to verify the reproducibility of the assessment of plasma cell infiltration (PC%) in bone marrow aspirates, used to collect cells for LI determination, and in bone marrow biopsies, used for AgNORs evaluation. Although an overall good correlation was observed between PC% in biopsies and aspirates (r=0.58, p=0.001), the ratio between PC% in biopsies and in aspirates ranged form 0.35 to 7.5. Only 17 patients (38.6%) were within the 0.5-1.5 range. A positive correlation between LI and AgNORs was observed in these patients (r=0.68, p=0.003), whereas the correlation was lost in patients with higher ratio between PC% in biopsies and in aspirates (r=0.08, p=0.69). The prognostic significance of AgNORs was confirmed by survival analysis, showing a reduced survival for patients with high (>4.4) AgNOR counts (14 months vs 35 months, p=0.004). The AgNORs analysis therefore allows the simultaneous evaluation of myeloma cell infiltration, degree of differentiation and kinetics of growth in bone marrow biopsies. AgNOR counts deserve to be included in the procedures for diagnosis and prognostic evaluation of myeloma patients.

Biopsy↗
PubMedDate not supplied

Therapy and survival in male breast carcinoma: A retrospective analysis of 50 cases.

The relationship between therapy and overall survival was retrospectively investigated in 50 patients with primary male breast carcinoma. Forty-five had radical or modified radical mastectomy and 5 simple mastectomy. Thirty-five received adjuvant post-operative therapy, including radiation, hormone and chemotherapy, given separately or in combination. The mean follow-up period was 67 (range, 1-230) months. The median survival was 33 months for patients receiving surgery alone and 86 months for those who also had adjuvant therapy (p=0.003). No difference in survival was found between simple or radical/modified radical mastectomy, nor among the various types of adjuvant therapy. Adjuvant therapy was most effective in large size, node positive and poorly differentiated tumors, and retained independent prognostic significance in multivariate analysis. With the limitation due to the small number of cases, our data suggest that adjuvant therapy may improve survival in males with cancer of the breast.

Adult↗