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Biomedical subjects

A Pierre

Publications and source records attributed to A Pierre.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Measurement of recA protein induction in Salmonella typhimurium: a possible biochemical test for the detection of DNA damaging agents.

RecA protein was purified from S. typhimurium and its concentration was measured in crude extracts by an immunoradiometric assay. The dose-response relations and the kinetics of recA protein induction following treatment of the cells with ultra-violet light, nalidixic acid, mitomycin C, and cisplatin were studied in E. coli and S. typhimurium. The recA protein amplification was complete in a few hours and was stable for at least 3 hours. Dose-response curves showed a linear region for low doses of all the inducer agents tested. This direct relation between the recA protein level and the amount of inducer agent allows the quantification of the recA protein inducing potency of chemicals. The recA protein amplification was very sensitive to low doses of inducer agents: an UV dose of 0.25 J/M2, 500 ng of NAL or 50 ng of MMC induced a two-fold increase in cellular recA protein content. In addition, the measurement of RecA protein induction did not require the survival of the cells. These observations led us to suggest a new biochemical assay for detecting DNA damaging substances by the direct measurement of the recA protein level following treatment of the cells.

Antibiotics, Antineoplastic↗
PubMedDate not supplied

The insulin-like growth factor axis in cell cycle progression.

Emerging evidence suggests that members of the Insulin-like Growth Factors (IGFs) family, including IGF-I, IGF-II, the IGF-I receptor (IGF-IR), and the IGF-binding proteins (IGFBPs) play a central role in the development and progression of cancer. Cancer cells exhibit an increased and deregulated proliferative activity. Abnormalities in many positive and negative modulators of the cell cycle are also frequent in many cancer types. Recent advances in the understanding of cell-cycle control mechanisms have been applied to outline the molecular mechanism through which IGFs regulate cell cycle progression. In this review, we will provide a brief overview of the role of the IGF system as a regulator of some components of the cell cycle.

Animals↗