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A R Morales

Publications and source records attributed to A R Morales.

At least 37 records · Page 2Linked to original sources
PubMed1984

Prekeratin immunohistochemistry in the diagnosis of undifferentiated carcinoma of the nasopharyngeal type.

We investigated the value of prekeratin immunostaining in establishing the diagnosis of undifferentiated carcinoma of the nasopharyngeal type (lymphoepithelioma). As in squamous cell carcinoma of the nasopharynx, all seven lymphoepithelial carcinomas stained for prekeratin whereas other look-alike but histogenetically different neoplasms (malignant lymphoma, esthesioneuroblastoma, and small round cell sarcomas) did not stain. In addition to confirming the squamous epithelial nature of lymphoepithelioma, our findings indicated that immunoperoxidase staining for prekeratin is a useful diagnostic tool in the differential diagnosis of nasopharyngeal carcinomas in general and lymphoepithelioma in particular.

Animals↗
PubMed1984

Maximizing the effectiveness of the autopsy in cases of sudden death.

Autopsies are essential in the investigation of sudden unexpected deaths. To maximize the effectiveness of the autopsy in these cases, the pathologist must assess the circumstances leading to the person's death, should be knowledgeable of the diseases most often responsible for sudden death, and should use dissection techniques most appropriate for their disclosure. In adults, the great majority of sudden deaths are cardiogenic and related to diseases of the coronary arteries, cardiac valves, or myocardium. In infants and young children, however, extracardiac diseases are prevalent in the causation of sudden deaths. Approximately one third of these are inexplicable (sudden infant death syndrome).

Adult↗
PubMed1983

Carcinoembryonic antigen in normal, preneoplastic and neoplastic gallbladder epithelium.

Carcinoembryonic antigen (CEA) was found in normal epithelium, premalignant lesions, and all histologic types of carcinomas of the gallbladder. Immunocytochemical staining for CEA was limited to the apical surface of normal epithelium. In premalignant lesions and in carcinomas, the staining was more extensive, occurring within the cytoplasm and gland secretions. These findings show that the presence and the pattern of distribution of CEA in normal and neoplastic gallbladder epithelium closely resemble that in other gastrointestinal epithelia.

Adenocarcinoma↗
PubMed1983

Thyroglobulin in carcinoma of the thyroid: an immunohistochemical study.

To determine the value of thyroglobulin as an immunohistochemical marker for thyroid neoplasms, we studied 42 primary thyroid carcinomas, 38 metastatic carcinomas, and four sarcomas involving the thyroid gland. All follicular and papillary carcinomas, regardless of their morphologic variation, stained positively for thyroglobulin, whereas the medullary carcinomas, metastatic tumors, and sarcomas showed negative staining reactions. The only small-cell variant of follicular carcinoma and ten of 14 spindle and giant cell carcinomas showed the lowest thyroglobulin reactivity. It is concluded that immunohistochemical demonstration of thyroglobulin is a sensitive and specific method of identifying thyroid carcinomas of follicular cell origin.

Carcinoma↗
PubMed1983

Immunoreactive prekeratin in Brenner tumors of the ovary.

In an attempt to further characterize benign, proliferating, and malignant Brenner tumors, immunoperoxidase staining for prekeratin was undertaken in a group of these neoplasms as well as in a variety of histologically different tumors of the ovary. All Brenner tumors reacted positively for prekeratin, whereas none of the other epithelial or sex cord-stromal tumors of the ovary stained. On the basis of these findings, we conclude that prekeratin might be a useful marker for differentiating malignant Brenner tumors from histologically similar, poorly differentiated ovarian neoplasms.

Brenner Tumor↗
PubMed1983

Immunohistochemical markers for prostatic cancer.

In a patient with an unclassifiable primary or metastatic neoplasm, with or without a history of prostatic cancer, immunostaining for PA or PSAP may prove invaluable. The procedure is simple, rapid, inexpensive, and extremely accurate in demonstrating the prostatic origin of tumors. It should be noted however, that the specificity of results is entirely dependent upon the specificity of the primary antibody, which should be meticulously defined before the procedure is used for diagnostic purposes.

Acid Phosphatase↗
PubMed1983

Prekeratin in spindle cell tumors of the skin.

A variety of benign and malignant skin lesions was stained for the presence of prekeratin using an unlabeled antibody peroxidase-antiperoxidase method and an antibody raised against human prekeratin protein. No prekeratin could be detected in benign or malignant lesions derived from melanocytes, and prekeratin could not be found in atypical fibroxanthoma, Kaposi's sarcoma, angiosarcoma, dermatofibrosarcoma protruberans, and leiomyosarcoma. Paget's cells did not contain prekeratin. Varying staining intensities were observed in keratinocytic atypical hyperplasias. Of particular interest is the observation that many spindle cell lesions that were difficult to identify with 100% assurance from routine stains could be positively identified as squamous cell carcinoma after prekeratin was found in the atypical cells.

Animals↗
PubMed1982

Paget's disease of the skin. A unifying concept of histogenesis.

Utilizing the peroxidase antiperoxidase technique we explored the presence and the distribution of carcinoembryonic antigen (CEA) in seven cases of mammary and sixteen of extramammary Paget's disease of skin. In every case positive immunostaining was observed in all the Paget's cells and the underlying tumor (where one was present), whereas intervening keratinocytes and melanocytes did not stain. CEA was also present in the cells and secretions of normal eccrine and apocrine glands. Our observations confirm that Paget's cells are of glandular origin.

Adenocarcinoma↗
PubMed1982

Carcinoembryonic antigen in sweat-gland carcinomas.

Using standard immunoperoxidase techniques, four cases of malignant sweat-gland carcinoma were examined for the presence of carcinoembryonic antigen (CEA). This substance was universally present in these lesions in spite of their wide spectrum of histologic features. CEA was mainly localized within the cytoplasm of tumor cells and in the lumina of neoplastic glands. The findings indicate that the presence of CEA in malignant skin adnexal tumors could help to identify and categorize these lesions as derived from eccrine or apocrine adnexal epithelium.

Adenocarcinoma↗
PubMed1982

Hemangioblastomas: histogenesis of the stromal cell studied by immunocytochemistry.

Twenty-one cases of hemangioblastoma from the cerebellum, spinal cord and retina were studied using the unlabeled antibody peroxidase-antiperoxidase technique with antibodies directed against glial fibrillary acidic protein (GFAP) and factor VIII related antigen (VIIIR:Ag). In 19 of 21 cases studied with anti-GFAP, astrocytes were identified peripherally, and in 13 cases they were found centrally within the tumor. In no instance did stromal cells react positively for GFAP. Sixteen cases with anti-VIIIR:Ag antibody were examined, and in all cases many stromal cells showed positive staining. It is concluded that the stromal cells were of endothelial origin. The occasional stromal cells that other investigators have identified as reacting positively for GFAP may represent stromal cells capable of ingesting extracellular GFAP derived from reactive astrocytes within the tumor, or they may be lipidized astrocytes.

Antigens↗
PubMed1982

Polyacrylamide-bismuth for postmortem coronary arteriography.

High quality postmortem human coronary arteriograms are obtained by injecting an acrylamide-bismuth mixture into the isolated aortic root under physiologic pressure. The acrylamide advances to the terminal size (20 micrometer.) arterioles, polymerizes, and remains within the vessels during the processing of tissue for histologic studies. Because the polyacrylamide does not affect the arterial wall morphology or the surrounding myocardium, the acrylamide-bismuth injection technique offers the clarity of the cast-corrosion procedure for postmortem radiograms fo the epicardial and intramural coronary arteries and adds the capability for histologic studies of arterial and myocardial tissues.

Acrylic Resins↗
PubMed1981

Prostatic-specific antigen: an immunohistologic marker for prostatic neoplasms.

Antiserum to a human prostate-specific antigen was raised in a rabbit and utilized by immunoperoxidase staining to evaluate its potential value as a diagnostic histologic marker for tumors of prostatic origin. All primary and metastatic prostatic malignancies reacted positively, whereas nonprostatic neoplasms did not stain with this procedure. This is the first immunohistochemical marker for prostate gland epithelium which does not represent prostatic acid phosphatase.

Adenocarcinoma↗
PubMed1981

Early electrophysiologic and anatomic alterations in cat ventricular muscle after coronary artery ligation.

The effect of coronary artery ligation on electrophysiologic properties of cat ventricular muscle cells was studied. Depression of resting potential, action potential rate of rise and amplitude was observed in infarcted cells, 30 min to 5 days after ligation. Action potential duration was markedly shortened in acute stages (30-120 min) but gradually lengthened to above control by 48 h. Anatomic sequelae included oedema, loss of fibre striation and cellular necrosis.

Action Potentials↗
PubMed1981

The making of a pathologist.

Numerous skills are required of pathologists today, skills so diverse that the traditional formats of residency programs no longer are functional. The author of this article explains what practice in pathology entails, the knowledge of which is essential if residents are to receive the proper training for practice in the field.

Internship and Residency↗
PubMed1981

Kaposi's sarcoma: immunohistologic evidence for an endothelial origin.

Factor VIII-related antigen (VIIIR-Ag), a marker for endothelial cells, was used to explore the histogenesis of Kaposi's sarcoma. Formaldehyde-fixed, paraffin-embedded routine histologic sections from 37 cases of Kaposi's sarcoma and from 16 other spindle-cell tumors of the skin were stained for the presence of VIIIR-Ag, using an immunoperoxidase technique. Positive reactions were noted in all cases of Kaposi's sarcoma regardless of their histologic variations. Although the intensity of reaction was greater in cells that lined vascular channels and blood-filled clefts, many intertwining spindle cells contained VIIIR-Ag in their cytoplasm as well. Since VIIIR-Ag is elaborated only by endothelial cells and megakaryocytes, positive immunologic reaction for this factor in the cells of Kaposi's sarcoma strongly supports an endothelial derivation.

Antigens↗