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Biomedical subjects

A Réffy

Publications and source records attributed to A Réffy.

At least 91 records · Page 5Linked to original sources
PubMed1979

Myofibrillar proteins, enzyme activity and fibre types in human skeletal muscle.

The myofibrillar proteins and relative LC-3 content of myofibrils prepared from five different, left and right muscles of the human forearm were studied by electrophoresis in 10% SDS-polyacrylamide gels followed by densitometry. The specific K-activated ATPase activity of the myofibrillar myosin of the myofibrils was determined and the distribution of the fibre types was analyzed by histochemical methods. The qualitative pattern of the myofibrillar proteins was found to be identical in the different muscles; in the other parameters, however, a variation in a narrow range was observed. The relative LC-3 content, the ATPase activity and the type II fibre content of the right side muscles were always higher than those of the corresponding left side muscles.

Adult↗
PubMed1979

[Myocardial damage in hemorrhagic hypotension].

Damage to the myocard caused by the loss of blood was studied in experiments on dogs. After long-lasting hypotonia focal lesions of the myocard of degenerative type have been found. Desorganization of the myofilaments and invagination of the disc could be seen in the area of the intercalar discs. Filaments in the other regions have not shown any alterations. In the mitochondria hypoxic lesions were revealed. One part of the alterations are not reversible. After 48 hours of survival focal necrosis with hyper contraction and calcification could be observed. It was surrounded by cellular infiltration containing mononuclear cells. Cellular necrosis revealed, may lie on the basis of the central lesion of the circulation appearing after long-lastng hypotonia.

Animals↗
PubMed1979

[Changes in the skeletal musculature due to tendon rupture].

Alterations of the sceletal muscles after spontaneous rupture of the tendon were studied. It was established that after the rupture of the tendon in the muscle activity of the succinedehydrogenase, myofibrillar ATP-ase, creatinphosphokinase and aldolase markedly decreases. After the rupture of the tendon alteration of the proportion of various types of fibers may also be observed i.e. the rate of the fibres of type I considerably decreases. Electron microscopic findings indicate that alterations of the musculature are diffuse first of all elements of contractility are damaged: atrophy and rupture of them can be seen. Fibres of normal ultrastructure were not found at all, which indicates that both types of fibres I and II are equally damaged.

Adult↗
PubMed1978

[Specific fibre compositon of human hand and arm muscles].

The authors examined the fiber composition of the finger, hand, and arm muscles. It is established that the type of fiber composition is not influenced by the mechanical employment of the hand- and arm muscles. The fiber composition of the finger muscles, however, is different in the two hands, in that the amount of fiber type II is significantly higher than that of the fiber type I in the dominant hand. In case of the non-dominant hand the fiber type I is predominant.

Arm↗
PubMed1977

The role of free fatty acids in pulmonary fat embolism.

Pulmonary embolism was produced in rabbits by the intravenous injection of 0-5 ml/kg body-weight of glycerol trioleate and mineral oil. Lung weight, pulmonary total lipid, esterified and free fatty acids content increased in both groups, but the concentration of free fatty acids increased only in the animals injected with neutral fat. Mineral oil injection produced stasis and haemorrhage; neutral fat produced exudation of serous liquid into the alveoli and an inflammatory reaction. There are definite differences between the pulmonary effects of mineral oil embolism and neutral fat embolism. The observations support the assumption that pulmonary changes in fat embolism are due to the toxic effect of free fatty acids liberated from the embolized neutral fat.

Animals↗
PubMed1977

[Changes in the granula of type II pneumocytes corresponding to the oxygen content of the air].

The morphology of granula of type II-granulocytes from lungs of rabbits and rats was studied. In pneumocytes of rabbits dense bodies predominated with high lipoprotein content, whereas in rats the majority had lamellar structure. Under treatment with hyperbaric oxygen the granula showed increased lipoprotein content, suggesting that the emanation of the content of granula to the alveolar surface is inhibited. Under hypoxia the release from the granula was enhanced, and after 16 hours of hypoxia the amount of granula was decreased as well as their lipoprotein content.

Animals↗
PubMed1977

Intramitochondrial calcification and myocardial inclusions in the rabbit due to anoxic hypoxia.

Intramitochondrial calcium granules and calcification of the mitochondrial cristae were examined in myocardial cells of rabbits in acute anoxic hypoxia, under normal circulation. The alterations were noted first after 2 hours of hypoxia, and became progressively larger and more ubiquitous with increasing periods of anoxic hypoxia. After 8 hours or longer hypoxia intramitochondrial "cristalloids" were found in some mitochondria.

Acute Disease↗
PubMed1976

Histochemical and ultrastructural alterations of the duodenum in acute hypoxia.

Laboratory animals were kept for 2, 8 and 16 hours in a pressure chamber, the air of which contained 8% O2 and 92% N2. Histochemical and ultrastructural examinations revealed the following duodenal alterations: 1. The alkaline phosphatase activity of the epithelium and glandular epithelium showed no alteration; the acid phosphatase activity was slightly increased in hypoxia. 2. The succinic dehydrogenase and cytochrome C oxidase activities of the glandular epithelium showed a marked decrease. 3. Two hours of hypoxia led to destruction of the microvillous epithelium. Prolonged hypoxia resulted in the destruction of the microvilli as well as of the cuticula. 4. Hypoxia of short duration had no damaging effect on glandular epithelial cells. After 8 to 16 hours of hypoxia, glandular secretion was reduced and the epithelial cells were evacuated. From the findings of the present investigation it is concluded that the decrease in the production of protective intestinal juice, due to the damaging effect of hypoxia on the epithelium and glandular epithelium as well as on the mitochondria, and the increase in the absorption of the intestinal content should be considered responsible of the additional damages to the intestinal epithelium.

Acid Phosphatase↗
PubMed1975

Light and electron microscopic studies of the pulmonary alveolar surfactant.

22 methods of light and electron microscopy have been tested for the identification of the pulmonary alveolar surfactant. Among the non electron microscopic methods Romhányi's anisotropic staining with toluidine blue was the most serviceable. In addition to simple performance it allows the identification of the smallest alteration -- not to be identified by other methods -- of the alveolar surfactant. Among the electron microscopic technics Ruthenium Red staining and Dermer's tricomplex method yielded the best results. Given that different chemical components of the alveolar surfactant will be identified by these 2 methods they supplement each other favourably. In the study of human cadaver material Baker's phospholipid reaction and Romhányi's anisotropic toluidine blue reaction is being used.

History, Ancient↗