Biomedical subjects
A Rüter
Publications and source records attributed to A Rüter.
Comparative colorimetry in cytophotometric measurements of azure B-eosin Y-stained and Giemsa-stained blood cell smears.
The advantages of the Commission Internationale de l'Eclairage (CIE) color measurements and the use of the standardizable purified azure B-eosin Y stain, as compared to the commonly used commercial Giemsa stain, were studied. Color measurement and analysis according to the CIE standards allows the comparison of color measurements from different slides stained by either the same or different procedures. Moreover, measurements from different cytophotometric systems can be compared. The results can also be directly correlated with human visual subjective color estimates and thus used to quantify the human observations. The color information in the air-dried cell specimens, which differs from the color characteristics of the components of the staining solution, was measured and analyzed.
[Femoral neck fractures in the child--therapy and results].
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[Shoulder prosthesis in traumatology].
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[Biomechanical examination of stress distribution in the femoral neck for varus-implanted and valgus-implanted cups (author's transl)].
Femoral neck fractures occur after cup-prosthesis implantation and inadequate trauma. By reaming the head the tension stress in the lateral tension banding system of the femoral neck is reduced. Therefore high unphysiological bending moments occur in the medial trabecular system, which resists only high pressure forces. Our investigations showed that the unfavourable change in the stress distribution in the femoral neck is worse for the varus- than for the valgus-implanted cup. This could be the cause for the femoral neck fractures after cup implantation and inadequate trauma.
[Results of corrective osteotomies of the radius base].
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[Additional measures in subcapital rotation osteotomy for management of habitual shoulder dislocation].
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[Bone and cartilage injuries of the knee joint].
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Relationship of ploidy and chromatin condensation in the rat liver, moreover a comparison of the nuclear texture in sections and touch preparations.
In touch preparations and tissue sections from normal rat hepatocytes and alpha-amanitine- and actinomycin D-poisoned liver cells the extent of chromatin condensation relatively to the degree of ploidy and the chromatin distribution were studied by means of computer aided cytophotometry. It could be found, that the relationship of the condensed and decondensed chromatin is independent of the degree of ploidy and first of all dependent upon the intoxicant. Therefore, the extent of chromatin condensation can be utilized also in sections as additional parameter for automated cell screening.
Color correction of understained and overstained leukocytes using the chromaticity coordinates of the neighboring erythrocytes to compensate for staining artifacts.
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[In vivo synthesis of cartilage after transplantation of chondrocytes in animal experiments (author's transl)].
Long-term results after cartilage transplantation are not satisfactory owing to poor viability of the chondrocytes. In vitro experiments with isolated fetal and neonatal rabbit chondrocytes revealed a much better proliferative ability compared with adult cartilage derived cells. Cryopreservation of cell suspensions did not decrease the proliferative ability. In in vivo experiments, cultured neonatal, fetal, and cryopreserved rabbit chondrocytes were tested in transplants and compared with cell-free controls or radiated chondrocytes. These chondrocytic transplants showed good viability and seemed to synthesize cartilage matrix, which could be stained with safranin, whereas only fibrous tissue was found in controls.
[Isoelastic prostheses for the shoulder].
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[Treatment of pathological vertebral fractures].
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Computer aided analysis of chromatin network and basophil color for differentiation of mononuclear peripheral blood cells.
Computer aided differentiation of plasmoblasts, Pfeiffer cells, immunoblasts, lymphocytes and centrocytes is achieved with the parameters of chromatin network arrangement and structure, and multispectral cytoplasm color. The digital methods involve: (a) segmenting the nuclear image into topographic sections and analyzing the optical density distribution from the chromatin in these sections; (b) determining the nuclear structure with a 7 x 7 median filter, gradient filter and contour following algorithms; and (c) clustering two-dimensional chromatic data from panoptically stained cellular components. The parameters reported here are a subset of those needed for the automated diagnosis of many hematologic diseases especially the leukemias.
Automated quantitative analysis of single and double label autoradiographs.
A method for the analysis of silver grain content in both single and double label autoradiographs is presented. The total grain area is calculated by counting the number of pixels at which the recorded light intensity in transmission dark field illumination exceeds a selected threshold. The calibration tests included autoradiographs with low (3H-thymidin) and high (3H-desoxyuridin) silver grain density. The results are proportional to the customary visual grain count. For the range of visibly countable grain densities in single labeled specimens, the correlation coefficient between the computed values and the visual grain counts is better than 0.96. In the first emulsion of the two emulsion layer autoradiographs of double labeled specimens (3H-14C-thymidin) the correlation coefficient is 0.919 and 0.906. The method provides a statistical correction for the background grains not due to the isotope. The possibility to record 14C tracks by shifting the focus through the second emulsion of the double labeled specimens is also demonstrated. The reported technique is essentially independent of size, shape and density of the grains.
[Meniscectomy (author's transl)].
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[In vitro study of isolated chondrocytes concerning the prognosis of transplanted cartilage].
In order to find out reliable criteria to conclude on the prognosis of transplanted cartilage the quality of isolated rabbit chondrocytes was assayed in vitro. Using a semisolid medium a culture system is introduced, which is able to detect colony forming units of chondrocytes. Furthermore their proliferative and differentiating capacity was investigated. Colony formation in vitro follows a strict dose response relationship. An inverse relationship was observed between the age of the donor and the colony incidence in vitro. Cells from neonatal cartilage could be induced to grow as fibroblastic colonies in the presence of conditioned medium from fibroblastic monolayer cultures. No effect, however, could be observed with chondrocyte derived conditioned medium.
[Functional results of primary talectomy].
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