Biomedical subjects

A Raczyńska

Publications and source records attributed to A Raczyńska.

At least 19 recordsLinked to original sources

PubMed1996

[Urease test, microbiologic tests, histologic and serologic tests for the evaluation of Helicobacter pylori infections in persons with peptic ulcer and gastritis].

The presence of Helicobacter pylori in the gastric mucosa of 150 patients with gastric, duodenal ulcer and endoscopic gastritis was investigated by rapid urease-based test, culture, histological examination and serology. Overall, 78% were positive for Helicobacter pylori by rapid urease-based test, 69.3% by culture, 53.7% by histological staining and 93.2% by serology (p < 0.001). The frequency of Helicobacter pylori was higher in patients with gastric or duodenal ulcer compared with those with endoscopic gastritis and those after gastric resection. However, the differences were significant only with reference to rapid urease-based test, (p < 0.02). The EIA (Roche) serological test had the highest sensitivity and the lowest specificity. This indicates the seed for serological kit to be independently evaluated on the population to be studied. The rapid urease-based test was simple, rapid and inexpensive, and it was more sensitive and equally specific compared with culture and Giemsa stain.

Adolescent↗

PubMed1996

[Morphologic changes of gastric mucosa in Helicobacter pylori infection in patients with peptic ulcer disease and chronic gastritis].

The present study was carried out to value the histological method of detection of Helicobacter pylori in gastric mucosa and to analyse the pathological changes of the Helicobacter pylori-positive patients with duodenal and gastric ulcer and with chronic gastritis. The studies population consisted of 150 patients, 76 F and 74 M. The samples were taken during gastroscopy from the prepyloric region and gastric body. After routine histological procedure they were stained HE and by Giemsa method for detection of H.pylori. The latter was identified in 56.6% of of the patients with duodenal ulcer, in 54.8% of those with gastric ulcer, in 17.6% of the patients after partial gastrectomy and in 46.9% of patients suffering from chronic gastritis. The increased density of H.pylori (third grade) was detected in the cases with clinical diagnosis of duodenal and gastric ulcer and erythematous endoscopic gastritis and was correlated with the histological picture of chronic active gastritis, which was characterized by diffuse mixed cellular exudation, lesion of the superficial epithelial, mucosal erosions and by foveolar hyperplasia. We suggest that this morphological picture corresponded to the active phase of Helicobacter infection. We conclude, that the histological method by Giemsas staining is effective especially in the acute phase of H.pylori infection and represents high diagnostic value and may be used in monitoring during the antibacterial (anti H.pylori) treatment. There is the correlation between massive density of H.pylori observed in gastric mucosa and chronic active gastritis.

Adolescent↗

PubMed1996

[Early results of treating Helicobacter pylori infections in patients with gastric ulcer and gastritis].

In the present study, the effectiveness of a triple therapy for eradication of Helicobacter pylori was evaluated. Therapy consisted of 120 mg tripotassium dicitrato bismuthate q.d.s. for four weeks, 500 mg amoxycillin q.d.s. and 500 mg metronidazole t.d.s. for two weeks. In 77 Helicobacter pylori-positive patients with duodenal ulcers (n = 32), gastritis (n = 18) and after gastric resection (n = 7), rapid urease-based test, culture, histology and serology were used to confirm the eradication, or relapse. The overall eradication rate was 75.3%, ulcers were healed in 82.1% and an improvement of the endoscopic gastritis was observed in 75.3% of the patients. The eradication rates were not statistically different among the subgroups. 1-3 months after the treatment IgG titres had fallen by 25% and over in 67.5% of the patients irrespective of the success of bacterial eradication. Side effects, particularly diarrhoea and nausea, were common (53.2%) but mild. Only 6.5% of the patients' had to discontinue the treatment because side effects became intolerable.

Adult↗

PubMed1993

[Influence of antagonistic bacteria on quantity of staphylococci in the oral cavity].

The study was aimed at establishment whether antagonistic influence between physiological oral cavity bacterial flora and staphylococci do exist and whether they influence on quantity of staphylococci in oral cavity environment. In samples of saliva taken from group of 11-13 year old children and a group of adults of both sexes, determinations were performed regarding total number of bacteria, number and percentage of bacteria inhibiting in vitro growth of standard indicator strain Staphylococcus aureus Oxford 209P and total number and percentage of staphylococci (together coagulase-positive and/or coagulase-negative). It was found that in each saliva sample bacteria inhibiting the indicator strain are present and their mean percentage is significantly higher in children than in adults and amounts respectively 47.1 +/- 28.5 and 35.0 +/- 20.3 (p = 0.0316). Higher percentage of antagonistic bacteria in children is associated with lower percentage of staphylococci in bacterial flora of saliva. Statistical analysis has confirmed existence of negative correlation, both linear and ranking, between the number of antagonistic bacteria and percentage of staphylococci in the saliva. This correlation is more strongly expressed and significant in children than in adults and linear correlation coefficient of Pearson amounts respectively to r = 0.7521 (p < 0.0005) and r = 0.3325 (p < 0.05). These results indicate that antagonistic bacterial flora is a significant factor limiting quantity of staphylococcal population in human oral cavity, especially in children.

Adolescent↗

PubMed1993

[Influence of antagonistic flora on quantity of staphylococci in the oral cavity].

The study was aimed at establishment, whether in oral cavity co-inhabit bacteria demonstrating antagonistic properties against staphylococci and whether this phenomenon may influence the size of staphylococcal population in this environment. Two locations of bacteria in oral cavity were investigated--saliva and tooth plaque. It was found that total quantity of staphylococci (both coagulase-positive and/or coagulase-negative) in saliva decreases with the age of investigated persons. Mean values amount respectively for children to 3.5 +/- 1.0 log CFU/ml and for adults to 2.9 +/- 0.7 log CFU/ml. This is statistically different (p = 0.0003). Amount of staphylococci in tooth plaques remains generally at the same level and amount to 1.4 +/- 0.6 log CFU/ml. In each tested sample of saliva and tooth plaque, presence of bacteria antagonistic to isolated from a given sample staphylococci, was detected. Among 2-4 year children and in adults, percentages of antagonistic bacteria against coagulase-positive staphylococci were higher than against coagulase-negative strains. Among children aged 5-6 and 11-13 years opposite proportions were found. Statistic analysis confirmed existence of negative correlations, both linear and ranking, between quantity of antagonistic bacteria and amounts of coagulase-positive and coagulase-negative staphylococci in the same tested samples of saliva and tooth plaque. These results suggest that antagonism between bacterial flora of oral cavity and staphylococci, is presenting one of factors limiting the quantity of staphylococci in this environment.

Adolescent↗

PubMed1993

[Production of glycocalyx by coagulase negative staphylococci isolated from the oral cavity].

The study was aimed at determination whether and how frequently coagulase-negative staphylococci persisting in human oral cavity possess the same feature. The material consisted of 255 strains of coagulase-negative staphylococci isolated from oral cavity of persons of both sexes and in differing age. Glycocalyx production by these strains was tested quantitatively by a colorimetric method. Glycocalyx was produced by 222 out of 225 tested strains (87.1%). For individual genera this frequency was following: epidermidis 164/186, hominis 14/14, warneri 12/13, saprophyticus 7/8, auricularis 4/5, haemolyticus 1/4, simulans 4/4, caseolyticus 2/2, xylosus 0/1 and for unidentified 14/18. Majority of tested strains produced only slight amounts of the glycocalyx. Only 46 out of the -20.7% of active strains (18.0% of all tested strains) produced large amount of glycocalyx. Among these strains, 37 belonged to the genus epidermidis, 6 to hominis and one each to warneri, simulans and auricularis. There was no correlation between amount of glycocalyx produced by individual strains and their number in the oral cavity. These results indicate that production of glycocalyx is a common feature of coagulase-negative staphylococci belonging to different genera and may appear with different intensity. Oral cavity may be a source of strains producing large amounts of glycocalyx and thus potentially pathogenic.

Coagulase↗

PubMed1993

[Antagonism between oral cavity streptococci and staphylococci].

The subject of this study was investigation of quantitative relations between streptococci, lactic acid bacilli and actinomycetes and staphylococci present in the oral cavity. It was found that in each tested sample of saliva streptococci are present which inhibit growth of an indicator strain Staphylococcus aureus 209P. Percentage of such streptococci varies from 11.1% to 100% (mean value 52.9 +/- 19.7%). There is a negative correlation between number of streptococci and staphylococci in samples of saliva delivered from the same oral cavity. Pearson's linear correlation coefficient was r = -0.7962 (p < 0.0001), and Spearman's correlation rank was rs = -0.7667 (p < 0.0001). Lactic acid bacilli were inhibiting growth of staphylococci only in conditions in which there were not neutralized by organic acids produced by these microorganisms. Actinomycetes had no influence at all on growth of these microorganisms. These results suggest that streptococci are responsible for antagonistic properties of oral cavity bacterial flora in relation to staphylococci. Numbers of streptococci conditions size of staphylococcal population in this environment. Staphylococci practically exert no antagonistic activity against cohabiting with them streptococci.

Adult↗

PubMed1990

[Elastolytic activity of staphylococci isolated from human oral cavity].

Elastolytic activity of 292 strains of staphylococci isolated from saliva samples obtained from children and adults was examined. It was found that 41% of coagulase-positive and 57.1% of coagulase-negative staphylococci were able to produce elastase. The difference in production frequency in relation to the ability to produce coagulase was statistically significant (p less than 0.01). Among coagulase-negative strains in addition to S. epidermidis, S. hominis, S. saprophyticus, S. haemolyticus, S. simulans, S. capitis, S. warneri and several unidentified strains were able to produce elastase.

Adult↗

PubMed1989

[Bacteriocinogenic properties of Staphylococci isolated from the oral cavity].

Two hundred ninety two staphylococcal strains were isolated out of 130 saliva samples taken from children and adults, among which 116 were coagulase-positive and 176 coagulase negative. Bacteriocinogenic activity against Staphylococcus aureus strain Oxford 209P was found in 13 (4.5%) of the strains only. On the other hand, when a set of 15 sensitive staphylococcal strains selected by cross checking was used for the study 260 (89.0%) strains were found to be bacteriocinogenic. It was found that a higher percentage of coagulase-positive staphylococcal strains is sensitive to staphylococcins than of coagulase-negative strains. However, mean zone of inhibition is smaller in the case of former than of the latter strains. It was shown, that in the case of active strains a positive correlation exists between a percentage of coagulase-positive and negative strains inhibited by them and also between a percentage of all inhibited strains and a mean diameter if the growth inhibition zone. Simultaneous occurrence in saliva of two or more staphylococcal strains was found in 106 persons examined. In 93.4% of those cases coexisting strains did not show antagonistic properties: in remaining 6.6% despite of the number of simultaneously existing strains in oral cavity only one strain showed antagonistic properties against the remaining strains.

Adult↗

PubMed1986