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Biomedical subjects

A Rahi

Publications and source records attributed to A Rahi.

9 recordsLinked to original sources
PubMed2000

Genotyping human platelet alloantigens (HPA 1-5) in Saudis from Eastern Province, Saudi Arabia.

In this study we report for the first time the distribution of human platelet alloantigens (HPA) in Saudis. These antigens are implicated in the pathophysiology of alloimmune thrombocytopenia. We collected blood samples from 84 healthy male Saudi blood donors. DNA isolated by salting-out and ethanol precipitation was amplified for genes HPA 1-5 using the polymerase chain reaction/sequence specific primer method. We found high HPA-1 polymorphism similar to Caucasians. HPA-4 polymorphism in Saudis was, however, greater than in Caucasians, and more similar to that of the Japanese. These results suggest that both these two HPA systems may be clinically important in Saudis.

Antigens, Human Platelet↗
PubMed1988

Immunodiagnosis of ocular chlamydial infection.

The diagnostic roles of enzyme immunoassay and indirect immunofluorescence tests were studied in 99 patients with various grades of active trachoma and in 72 patients with inactive trachoma. Normal individuals and patients with non-specific chronic conjunctivitis were used as a control group. Chlamydial antigens were detected in the conjunctival swabs from a large proportion of patients with severe or moderate trachoma. Cases with 'antigen-negative' but clinically severe trachoma showed presence of specific IgG in the blood, thus indicating that serological tests may complement the ELISA test for the detection of chlamydia in infected tissues. The nature of cellular reaction in the conjunctiva seems to be related to the severity of trachoma and presence of the infective organism. 'Antigen-negative' trachoma thus represents either an error of diagnosis or limited sensitivity of currently available immunologic techniques, or a predominantly hypersensitivity state triggered by transient tissue parasitism. Recombinant DNA technology and chronobiologic study of infiltrating lymphocytes are likely to provide some insight in such cases.

Adolescent↗
PubMed1980

Antibodies to skeletal muscle actin and their reactivity with malignant melanoma of the choroid.

Imprints of choroidal malignant melanoma cells were treated with serum containing antibodies to a purified preparation of actin derived from skeletal muscle. Evidence of a positive reaction, as shown by indirect immunofluorescence, substantiated an impression based on morphological criteria that choroidal melanoma cells contain actin protein. The significance of this protein in tumour biology is discussed, and a possible interference by antiactin antibodies in the immunodiagnosis of choroidal melanoma is highlighted.

Actins↗
PubMed1978

Immunological investigations in post-traumatic granulomatous and non-granulomatous uveitis.

Histopathological and immunological investigations have been performed in 16 cases of post-traumatic granulomatous (10) and non-granulomatous uveitis (6). Most cases of sympathetic ophthalmitis showed evidence of cell mediated immunity to uveoretinal antigens, though it was not possible to make a definitive diagnosis on immunological grounds alone. Three patients with post-traumatic non-granulomatous uveitis showed a positive immunological response to ocular antigens, and 2 of these later developed clinical evidence of sympathetic ophthalmitis, which suggests that post-traumatic non-granulomatous uveitis in such cases may represent a presympathetic (i.e., incipient) or modified stage of the disease.

Antigens↗
PubMed1978

Contractile proteins in retinal endothelium and other non-muscle tissues of the eye.

The present study is the first investigation to demonstrate, by employing the combined approach of immunologically and electron microscope methods, the presence of actin-like contractile proteins in the mammalian retina, the corneal epithelium and endothelium, the iris, and the ciliary body, and to confirm their presence in lens epithelium. This is also the first report to demonstrate by these methods the presence of microfilaments and intermediate filaments in retinal vascular endothelium. Since we have shown that actin filaments are especially abundant in immature retinal endothelial cells, the question of their function arises, and we have discussed their possible relevance to the closure of immature retinal vessels when exposed to hyperoxia.

Actins↗
PubMed1977

Keratoconus and coexisting atopic disease.

The association of keratoconus and atopic disease has been reported on several occasions but the only controlled clinical study that has so far been published found no evidence to support this view. Since it is now known that atopy is often associated with changes in various immunoglobulins, particularly IgE, it was considered desirable to determine the immunological profiles of a large series of keratoconus cases in order to seek evidence for coexistence of the two conditions in one individual. In this study of 182 cases of keratoconus a definite history of atopy was found in 35% compared with 12% in the matched control group. The serum IgE was significantly raised (P less than 0.001) in keratoconus and markedly so in those cases with associated atopic disease. Serum levels of IgG and IgM were also raised, but contrary to the findings of other observers IgA levels were normal. These findings suggest that atopic traits are more common in patients with keratoconus than in general ophthalmic patients.

Female↗
PubMed1977

Reticulum fibres in relation to retinal vessels.

Argyrophilic perivascular and intervascular fibres in the mammalian retina are shown by specific antireticulin immunofluorescence to consist of reticulin. The possible significance of these findings is briefly discussed.

Animals↗