PubMed Health⌕ Search

Biomedical subjects

A Raile

Publications and source records attributed to A Raile.

7 recordsLinked to original sources
PubMed1996

[Clinical electro-ophthalmology at the Max Planck Institute of the Frankfurt University Ophthalmology Clinic 1970-1991].

The survey shows the frequency and distribution of diseases evaluated by electroophthalmological methods. Patients with retinal diseases (51.2%) and those with diseases of the optic nerve (21.8%) were examined most frequently. In a high percentage these investigations lead to a clinically useful assessment: described as confirmation or exclusion of a clinical diagnosis, as establishing a possible differential diagnosis or clearing up formerly unknown aspects of a disease. In cases of hereditary retinal disorders only 11% remained unclear, with presumed optic neuritis only 6%. The importance of electroophthalmological investigations is there ability to assess functional deficits in the visual system especially in somehow more rare retinal and centrally located disorders, functional deficits of unknown origins or in general diseases including the visual system.

Cross-Sectional Studies↗
PubMed1989

Cortical and retinal refractory periods in the human visual system.

Refractory periods of the visual system were investigated in 12 healthy subjects by simultaneously recording retinal (ERG) and cortical (VEP) evoked electrical activity. Double-flash stimuli were presented at different interstimulus intervals, and response components evoked by the second flash were analyzed in detail, and related to psychophysical detection thresholds. With short interstimulus intervals ERG b-wave peak latencies were increased and b-wave amplitudes were significantly reduced, while P100 component latencies of the VEP were significantly influenced only at long interstimulus intervals. Regression analysis of the individual data as well as analysis of the retinocortical transmission times showed that the cortical latency changes were not simply caused by changes on the level of the retina. Additional influences of the interstimulus interval on nonretinal structures of the human visual system must be assumed. The subjective psychophysical detection thresholds were significantly higher than the threshold values at which reliable electrical or cortical response components could be elicited.

Adult↗
PubMed1983

Conditions for the enhancing effect of protease inhibitors on the concanavalin A induced thymidine response of murine lymphocytes.

Incorporation of [3H]-thymidine - [3H]-TdR - into concanavalin A (Con A) stimulated murine splenocytes and thymocytes was found to be enhanced by addition of certain concentrations of phenyl-methylsulfonylfluoride (PMSF), di-isopropylfluorophosphate (DFP), N-alpha-tosyl-L-lysyl-L-chloromethylketone (TLCK), and soybean trypsin inhibitor (SBTI). No enhancement could be observed when mononuclear cells of the peripheral blood were used, and a medium enhancement when thymocytes were applied. Furthermore, no enhancing effect of the protease inhibitors (PI) on the Con A response of murine splenocytes could be observed within the first 24 h of the culturing period. DFP, PMSF, and TLCK enhanced the Con A response to a similar degree, whereas SBTI was less effective. DFP and SBTI proved to be also effective when they were added after 15-24 h to the Con A cultures, if the cultures were harvested 48 h later. Removal of adherent and phagocytic spleen cells or reduction of the concentration of spleen cells shifted the effective DFP concentration to lower concentrations, whereas addition of adherent spleen cells caused a shift of the enhancing DFP amounts to higher concentrations. The data presented suggest that the enhancing effect of PI on the T cell response depends on the concentration of PI, the time of culturing and incubation, the PI used, the origin of the stimulated cells, and especially on the number of adherent and phagocytic cells. These findings might explain - at least in part - the different results on the effect of PI on the T cell response obtained in the past.

Animals↗
PubMed1982

Differential effect of low molecular weight alcohols on the Con A stimulation of mouse spleen cells.

Incorporation of [3h]thymidine ([3H]TdR) into concanavalin A (Con A)-stimulated murine splenocytes was found to be enhanced by addition of certain concentrations of ethanol, 2-propanol and acetone. The alcohol/acetone-induced enhancement of the Con A response was found to be accompanied by an increase of the percentage of living cells as assessed by trypan blue exclusion. Concentrations of ethanol and 2-propanol which caused maximum [3H]TdR uptake in Con A cultures were also found to lead to higher percentages of aggregated cells than in comparison to Con A cultures without alcohol. The data suggest that alcohols in certain concentrations are capable to achieve optimum Con A stimulation.

Alcohols↗