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Biomedical subjects

A Ranki

Publications and source records attributed to A Ranki.

At least 127 records · Page 7Linked to original sources
PubMed1980

Histochemical identification of human T lymphocytes from paraffin sections.

The alpha-naphthyl acetate esterase (ANEA) is a histochemical marker for human T lymphocytes in cell smears and frozen tissue sections. We have now applied the ANAE method to paraffin-embedded tissue sections. We first demonstrated with cytocentrifuged cell smears of blood leukocytes that the ANAE activity is preserved upon prolonged storage in formol calcium, Holt's buffer, acetone, xylene, and heat. When the tissue sections were similarly processed and embedded in paraffin, the ANAE positive (T) lymphocytes were identified by their distinct display of one or more reddish-brown reaction dots in the cell cytoplasm. ANAE positive mononuclear phagocytes were easioy distinguished from the T lymphocytes by their diffuse, sodium fluoride-sensitive pancytoplasmic reaction. The extension of the ANAE method to paraffin-embedded tissue sections with superior morphological integrity, makes it possible to apply it in practical biopsy pathology.

Biopsy↗
PubMed1978

Presence of alpha-naphthyl acetate esterase activity in human haematopoietic cell lines and in fresh biopsy specimens of lymphoma and myeloma.

We have previously shown that non-proliferating human T- but not B-lymphocytes contain demonstrable amounts of acid alpha-naphthyl acetate esterase (ANAE). The usefulness of this histochemical marker for the diagnosis and classification of malignant lymphoid tumors was investigated by use of a panel of established normal and malignant human haematopoietic cell lines and fresh biopsy cells from malignant lymphomas and myelomas. The results showed that not only the T-cell derived acute leukaemia lines, but also histiocytic lymphoma and myeloma lines and some of the lymphoma (Burkitt and lymphocytic) and non-neoplastic lymphoblastoid cell lines with B-cell surface markers expressed strong ANAE reactivity. Some but not all of the immunoglobulin producing myeloma and lymphocytic lymphoma biopsies were ANAE-positive. Inhibition experiments with sodium fluoride and E-600 demonstrated that although the T-lymphocyte specific esterase is predominantly of 'A'-type, the malignant lines contain also non-specific 'B' esterase and pseudocholinesterase. As the presence of the various esterases did not demonstrate any specific distribution pattern among he haematopoietic cell lines of different origin, we concluded that the ANAE marker is no longer T-specific when malignant lymphoid cells are considered, and that the usefulness of this marker in routine diagnostic work therefore is limited.

B-Lymphocytes↗
PubMed1977

Expression of the acid alpha-naphthyl acetate esterase marker by activated and secondary T lymphocytes in man.

Acid alpha-naphthyl acetate esterase (ANAE) activity is characteristic of resting human T lymphocytes. The expression of the ANAE marker by activated human T and B lymphocytes (blasts) and by corresponding 'secondary' lymphocytes has been investigated. Human blood lymphocytes were stimulated by selective T-cell (phytohemagglutinin (PHA) and concanavalin A (Con A)) or B-cell (Staphylococcus aureus strain Cowan 1) mitogens or in the mixed lymphocyte culture (MLC), and the percentage of blasts expressing the marker was quantiated. Whereas 95% of Con-A-activated blasts expressed the marker, approximately 25%-30% of MLC-activated blasts and only 10%-25% of PHA-activated blasts were ANAE-positive. After reversion to secondary lymphocytes, the PHA- and MLC-activated cells regained the ANAE activity, and more than 90% of the blast-derived secondary T lymphocytes were ANAE-positive. Only 2%-8% of blast cells activated by Staphylococcus aureus strain Cowan 1 were ANAE-positive. We therefore conclude that ANAE is not a reliable marker for T cells when activated cells (blasts) are considered.

B-Lymphocytes↗
PubMed1977

Lack of subclass selection during density purification of human lymphocytes.

The percentage of acid alpha-naphthyl acetate esterase (ANAE) marker-carrying lymphocytes (T-lymphocytes) was determined directly from the blood, after density purification of lymphocytes over Ficoll-Isopaque and removal of monocytes by iron powder plus magnet. The effect of Ficoll-Isopaque centrifugation was insignificant; neither did the iron powder plus magnet procedure increase the relative proportion of the T cells in these preparations. We therefore conclude that in optimal conditions there is no subclass-specific enrichment of T or B lymphocytes during the purification procedure.

B-Lymphocytes↗
PubMed1976

Identification of resting human T and B lymphocytes by acid alpha-naphthyl acetate esterase staining combined with rosette formation with Staphylococcus aureus strain Cowan 1.

We describe a method enabling the identification of both lymphocyte class and morphology from a single microscopic slide. As a marker for B cells we used surface immunoglobulin. The surface-Ig-carrying cells were rosetted after poly-valent anti-Ig treatment with Staphylococcus aureus strain Cowan 1 (StaCw) and the cells were cytocentrifuged onto a microscope slide. The lymphocytes forming rosettes with StaCw were identical with cells expressing surface Ig studied by fluorescein-isothiocyanate-conjugated anti-Ig. As a marker for T cells we used the acid alpha-naphthyl acetate esterase (ANAE) histochemical marker. The cell smears were first stained for ANAE and subsequently counterstained to distinguish also cell morphology. The ANAE-marker-carrying cells were all included in the population of lymphocytes forming rosettes with sheep erythrocytes. Thus both T and B lymphocytes could be simultaneously identified from a single microscopic slide, and we therefore recommend the method for routine clinical work.

B-Lymphocytes↗
PubMed1976

Identification of mouse T and B lymphocytes from cytocentrifuged cell smears.

A method enabling direct enumeration of mouse T and B cells from cytocentrifuged cell smears is described. The acid alpha-naphthyl acetate esterase (ANAE) staining was used as marker for T cells. The distribution of the ANAE marker-carrying lymphocytes closely followed the percentual distribution of T cells in spleen and lymph node. Electrophoresis analysis demonstrated that while more than 95% of resting spleen and lymph node T cells carried the ANAE marker, only less than 5% of the B cells were ANAE positive. The B cells (surface-bound Ig-carrying small lymphocytes) were identified by anti-Ig serum followed by rosetting of the B cells with Staphylococcus aureus strain Cowan 1 (StaCw). The ANAE marker-carrying cells did not overlap with the StaCw rosette-forming lymphocytes. Thus we conclude that the combination of StaCw rosetting and ANAE staining enables accurate identification of resting T and B cells from a single microscope slide. Ninety to 100% of Con A-activated blasts expressed the ANAE marker but only 60-85% of PHA and MLC-activated blasts were positive. Twenty to 33% of the blast cells stimulated by LPS expressed the ANAE marker. Thus the ANAE marker is not a reliable criterion for T cells in actibated state.

Animals↗
PubMedDate not supplied

Carbon dioxide (CO2)-laser therapy cures macroscopic lesions, but viral genome is not eradicated in men with therapy-resistant HPV infection.

BACKGROUND AND OBJECTIVES: We have evaluated the efficacy of CO2-laser in eradicating human papillomavirus (HPV) DNA from genitoanal skin lesions. STUDY DESIGN: Biopsies of 38 male patients with histologically confirmed HPV-infection after an average of 2 years of follow-up were analyzed. Post-treatment biopsies were obtained from all residual or recurrent HPV-suspect (acetowhite) lesions in 23 patients. RESULTS: After an average of three separate CO2-laser treatments, 15 of 38 patients were devoid of any clinical or acetowhite lesions. By in situ hybridization (ISH), the frequency of HPV-types 6/11 decreased from 52% to 26%, and HPV-types 16/18 decreased from 48% to 17%, respectively, in 23 patients biopsied twice. When ISH-negative biopsies were further analyzed with polymerase chain reaction (PCR) and southern blotting (SB) for HPV-16, HPV-types 16/18 were detected in a total of 65% of biopsies before CO2-laser therapy, and in 61% after the therapy. The cure rate achieved with CO2-laser was 39% (15/38) according to clinical, 61% (14/23) according to histopathological, and 26% (6/23) according to molecular biological criteria. The frequency of Bowenoid papulosis was reduced from 57% (13/23) to 17% (4/23). CONCLUSIONS: Although CO2-laser is ineffective in eradicating HPV genome from therapy-resistant penile warts, the treatment reduces the recurrence of atypical changes and visible warts.

Anus Diseases↗
PubMedDate not supplied

Comparison of four in situ hybridization methods, based on digoxigenin- and biotin-labelled probes, in detecting HPV DNA in male condylomata acuminata.

We have compared the efficacy of digoxigenin- and biotin-labelled probes in detecting HPV DNA by in situ hybridization on paraffin-embedded tissue sections of 57 male condyloma-suspect genital lesions. Each biopsy was hybridized with at least three of the following four methods: digoxigenin-labelled HPV DNA probes (Dig-HPV), biotinylated HPV-DNA probes (Bio-HPV), and two commercial methods (ViraType in situ and PathoGene), both based on biotinylated DNA probes. The hybridization products were visualized with colourigenic enzyme substrates. In most biopsies, the 4 methods gave equal results although cross-hybridization was most often found with the low-stringency ViraType method. Dig-HPV 6/11 probes gave positive results about twice as often as either of the commercial methods. No such difference, however, was found for HPV 16/18 probes. DNA of any type of HPV 6/11, 16/18 or 31/33/35 or 51 was detected in 28/43 (65%) of lesions showing condyloma acuminatum histology but in none of the 14 biopsies with no histological signs of HPV infection. In HPV-positive condylomata with no cellular atypia. HPV 6/11 was detected in 87% (13/15), and HPV 16/18 in 27% (4/15). In biopsies with cellular atypia, HPV types 6/11 were detected in 62% (8/13), HPV types 16/18 in 46% (6/13), and HPV types 31/33/35 or 51 in 50% (6/12). In about 50% of the biopsies where at least one hybridization method gave a positive result, either one of the commercial methods gave a negative result.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗