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Biomedical subjects

A Reeves

Publications and source records attributed to A Reeves.

At least 19 recordsLinked to original sources
PubMed2002

In vivo three-dimensional imaging of plants with optical coherence microscopy.

Achieving the ability to non-destructively, non-invasively examine subsurface features of living multicellular organisms at a microscopic level is currently a challenge for biologists. Optical coherence microscopy (OCM) is a new photonics-based technology that can be used to address this challenge. OCM takes advantage of refractive properties of biological molecules to generate three-dimensional images that can be viewed with a computer. We describe new data processing techniques and a different visualization algorithm that substantially improve OCM images. We have applied OCM imaging, in conjunction with these improvements, to a variety of structures of plants, including leaves, flowers, ovules and germinating seeds, and describe the visualization of cellular and subcellular structures within intact plants. We present evidence, based on detailed examination of our OCM images, comparisons to classical plant anatomy studies, and current knowledge of light scattering by cells and their components, that we can distinguish nuclei, organelles and vacuoles. Detailed examination of vascular tissue, which contains cells with elaborate wall structure, shows that cell walls produce no significant OCM signal. These improvements to the visualization process, together with the powerful non-invasive, non-destructive aspects of the technology, will broaden the application of OCM to questions in studies of plants as well as animals.

Arabidopsis↗
PubMed2001

Visual pattern encoding with weighted hermite polynomials.

The human visual system is spatially inhomogenous, and this property should be included in models of visual processing. We used weighted Hermite polynomials (WHPs) to encode and to characterize such inhomogenous processing. Simulations using an order-transfer-function (OTF) defined for each WHP order, at three spatial scales, provide elegant predictions of spatial frequency discrimination, of WHP order discrimination, and variations in two-point resolution and detection sensitivity with retinal eccentricity.

Computer Simulation↗
PubMed2001

MicroMeasure: a new computer program for the collection and analysis of cytogenetic data.

The ability to identify individual chromosomes in cytological preparations is an essential component of many investigations. While several computer software applications have been used to facilitate such quantitative karyotype analysis, most of these programs are limited by design for specific types of analyses, or can be used only with specific hardware configurations. MicroMeasure is a new image analysis application that may be used to collect data for a wide variety of chromosomal parameters from electronically captured or scanned images. Unlike similar applications, MicroMeasure may be individually configured by the end user to suit a wide variety of research needs. This program can be used with most common personal computers, and requires no unusual or specific hardware. MicroMeasure is made available to the research community without cost by the Department of Biology at Colorado State University via the World Wide Web at http://www.biology.colostate.edu/MicroMeasure.

Chromosome Banding↗
PubMed2000

Optical coherence microscopy. A technology for rapid, in vivo, non-destructive visualization of plants and plant cells.

We describe the development and utilization of a new imaging technology for plant biology, optical coherence microscopy (OCM), which allows true in vivo visualization of plants and plant cells. This novel technology allows the direct, in situ (e.g. plants in soil), three-dimensional visualization of cells and events in shoot tissues without causing damage. With OCM we can image cells or groups of cells that are up to 1 mm deep in living tissues, resolving structures less than 5 microm in size, with a typical collection time of 5 to 6 min. OCM measures the inherent light-scattering properties of biological tissues and cells. These optical properties vary and provide endogenous developmental markers. Singly scattered photons from small (e.g. 5 x 5 x 10 microm) volume elements (voxels) are collected, assembled, and quantitatively false-colored to form a three-dimensional image. These images can be cropped or sliced in any plane. Adjusting the colors and opacities assigned to voxels allows us to enhance different features within the tissues and cells. We show that light-scattering properties are the greatest in regions of the Arabidopsis shoot undergoing developmental processes. In large cells, high light scattering is produced from nuclei, intermediate light scatter is produced from cytoplasm, and little if any light scattering originates from the vacuole and cell wall. OCM allows the rapid, repetitive, non-destructive collection of quantitative data about inherent properties of cells, so it provides a means of continuously monitoring plants and plant cells during development and in response to exogenous stimuli.

Microscopy↗
PubMed2000

Theory of spatiochromatic image encoding and feature extraction.

We consider how to interpret, filter, and cross-correlate complex-value color (hue and saturation) images by using a single discrete Fourier transform: the spatiochromatic discrete Fourier transform. The model defines new types of spatiochromatic oriented sinusoidal gratings, termed rainbow gratings, which encode the variation of color over space. We demonstrate how color-opponent detectors observed within the vertebrate visual system can be easily defined by linear filters within this representation. This model also allows us to filter and detect both spatial and chromatic patterns in images by using a single cross-correlation procedure. In doing so, we explore a new form of the Cauchy-Schwartz inequality applied to complex-valued scalar products. Results demonstrate the power of this form of spatiochromatic matched filtering in detecting signals embedded in such a significant amount of noise that they are not visible to the unaided human eye.

Animals↗
PubMed2000

Rotating objects to determine orientation, not identity: evidence from a backward-masking/dual-task procedure.

The effects of picture-plane rotations on times taken to name familiar objects (RTs) may reflect a process of mental rotation to stored viewpoint-specific representations: the rotate-to-recognize hypothesis. Alternatively, mental rotation might be used after stored object representations are activated by a misoriented stimulus in order to verify a weak or distorted shape percept: the double-checking hypothesis. We tested these two accounts of rotation effects in object recognition by having subjects verify the orientations (to within 90 degrees) and basic-level names of 14-msec, backward-masked depictions of common objects. The stimulus-mask interval (SOA) varied from 14 to 41 msec, permitting interpolation of the SOA required for 75% accuracy (SOAc). Whereas the SOAc to verify orientation increased with rotation up to 180 degrees, the SOAc to verify identity was briefer and asymptoted at approximately 60 degrees. We therefore reject the rotate-to-recognize hypothesis, which implies that SOAc should increase steadily with rotation in both tasks. Instead, we suggest that upright and near-upright stimuli are matched by a fast direct process and that misoriented stimuli are matched at a featural level by a slightly slower view-independent process. We also suggest that rotation effects on RTs reflect a postrecognition stage of orientation verification: the rotate-to-orient hypothesis, a version of double-checking that also explains the well-known reduction in orientation effects on RTs when naming repeated objects.

Attention↗
PubMed2000

Intraosseous hematoma in a newborn with factor VIII deficiency.

We present an unusual case of an intraosseous hematoma in a newborn with a known bleeding disorder. This cephalohematoma was diagnosed shortly after birth, was entirely within the bony skull, and was in fact determined to be an intraosseous hematoma. The initial CT scans showed the unusual appearance and location of the lesion; later scans showed a significant amount of remodeling, with resolution of the hematoma. Although the coagulopathic diagnosis was independent of this finding, a bleeding disorder might be considered in other patients with similar CT findings.

Bone Diseases↗
PubMed1999

Synthesis of the nanomolar photoaffinity GABA(B) receptor ligand CGP 71872 reveals diversity in the tissue distribution of GABA(B) receptor forms.

A radioiodinated probe, [125I]-CGP 71872, containing an azido group that can be photoactivated, was synthesized and used to characterize GABA(B) receptors. Photoaffinity labeling experiments using crude membranes prepared from rat brain revealed two predominant ligand binding species at approximately 130 and approximately 100 kDa believed to represent the long (GABA(B)R1a) and short (GABA(B)R1b) forms of the receptor. Indeed, these ligand binding proteins were immunoprecipitated using a GABA(B) receptor-specific antibody confirming the receptor specificity of the photoaffinity probe. Most convincingly, [125I]-CGP 71872 binding was competitively inhibited in a dose-dependent manner by cold CGP 71872, GABA, saclofen, (-)-baclofen, (+)-baclofen and (L)-glutamic acid with a rank order and stereospecificity characteristic of the GABA(B) receptor. Photoaffinity labeling experiments revealed that the recombinant GABA(B)R2 receptor does not bind [125I]-CGP 71872, providing surprising and direct evidence that CGP 71872 is a GABA(B)R1 selective antagonist. Photoaffinity labeling experiments using rat tissues showed that both GABA(B)R1a and GABA(B)R1b are co-expressed in the brain, spinal cord, stomach and testis, but only the short GABA(B)R1b receptor form was detected in kidney and liver whereas the long GABA(B)R1a form was selectively expressed in the adrenal gland, pituitary, spleen and prostate. We report herein the synthesis and biochemical characterization of the nanomolar affinity [125I]-CGP 71872 and CGP 71872 GABA(B)R1 ligands, and differential tissue expression of the long GABA(B)R1a and short GABA(B)R1b receptor forms in rat and dog.

Affinity Labels↗
PubMed1999

Learning enhances adult neurogenesis in the hippocampal formation.

Thousands of hippocampal neurons are born in adulthood, suggesting that new cells could be important for hippocampal function. To determine whether hippocampus-dependent learning affects adult-generated neurons, we examined the fate of new cells labeled with the thymidine analog bromodeoxyuridine following specific behavioral tasks. Here we report that the number of adult-generated neurons doubles in the rat dentate gyrus in response to training on associative learning tasks that require the hippocampus. In contrast, training on associative learning tasks that do not require the hippocampus did not alter the number of new cells. These findings indicate that adult-generated hippocampal neurons are specifically affected by, and potentially involved in, associative memory formation.

Animals↗
PubMed1999

Distribution of crossing over on mouse synaptonemal complexes using immunofluorescent localization of MLH1 protein.

We have used immunofluorescent localization to examine the distribution of MLH1 (MutL homolog) foci on synaptonemal complexes (SCs) from juvenile male mice. MLH1 is a mismatch repair protein necessary for meiotic recombination in mice, and MLH1 foci have been proposed to mark crossover sites. We present evidence that the number and distribution of MLH1 foci on SCs closely correspond to the number and distribution of chiasmata on diplotene-metaphase I chromosomes. MLH1 foci were typically excluded from SC in centromeric heterochromatin. For SCs with one MLH1 focus, most foci were located near the middle of long SCs, but near the distal end of short SCs. For SCs with two MLH1 foci, the distribution of foci was bimodal regardless of SC length, with most foci located near the proximal and distal ends. The distribution of MLH1 foci indicated interference between foci. We observed a consistent relative distance (percent of SC length in euchromatin) between two foci on SCs of different lengths, suggesting that positive interference between MLH1 foci is a function of relative SC length. The extended length of pachytene SCs, as compared to more condensed diplotene-metaphase I bivalents, makes mapping crossover events and interference distances using MLH1 foci more accurate than using chiasmata.

Adaptor Proteins, Signal Transducing↗
PubMed1999

Two-dimensional multi-criterion segmentation of pulmonary nodules on helical CT images.

A multi-criterion algorithm for automatic delineation of small pulmonary nodules on helical CT images has been developed. In a slice-by-slice manner, the algorithm uses density, gradient strength, and a shape constraint of the nodule to automatically control segmentation process. The multiple criteria applied to separation of the nodule from its surrounding structures in lung are based on the fact that typical small pulmonary nodules on CT images have high densities, show a distinct difference in density at the boundary, and tend to be compact in shape. Prior to the segmentation, a region-of-interest containing the nodule is manually selected on the CT images. Then the segmentation process begins with a high density threshold that is decreased stepwise, resulting in expansion of the area of nodule candidates. This progressive region growing approach is terminated when subsequent thresholds provide either a diminished gradient strength of the nodule contour or significant changes of nodule shape from the compact form. The shape criterion added to the algorithm can effectively prevent the high density surrounding structures (e.g., blood vessels) from being falsely segmented as nodule, which occurs frequently when only the gradient strength criterion is applied. This has been demonstrated by examples given in the Results section. The algorithm's accuracy has been compared with that of radiologist's manual segmentation, and no statistically significant difference has been found between the nodule areas delineated by radiologist and those obtained by the multi-criterion algorithm. The improved nodule boundary allows for more accurate assessment of nodule size and hence nodule growth over a short time period, and for better characterization of nodule edges. This information is useful in determining malignancy status of a nodule at an early stage and thus provides significant guidance for further clinical management.

Algorithms↗
PubMed1998

The effect of photon noise on the detection of white flashes.

Thresholds for detecting brief, white, foveal test flashes drop abruptly within 0.2 sec of the offset of a white adapting field. The magnitude of the abrupt drop is proportional to the square root of field intensity (square root of I) correct for bleaching and dark light. Thresholds are then stable out to 1.6 sec for 200 msec tests, or recover only slightly for 20 msec tests. These results exclude some simple deterministic models in which Weber-like gain controls in the luminance pathway are assumed to recover exponentially in the dark, but can be explained parsimoniously if turning off the field abolishes photon-driven noise, improving the S/N ratio while leaving visual responsivity virtually unaltered. This theory was first put forward by Krauskopf and Reeves [(1980) Vision Research, 20, 193-196] for S-cone thresholds; it implies that the Weber law for increment thresholds is not due to a single gain control, but rather expresses the product of two distinct square root of I factors, adjustment of responsivity and photon-driven noise. Removal of the noise, not recovery of gain, permits thresholds to fall in early dark adaptation.

Adaptation, Ocular↗
PubMed1997

Simulated cataract does not reduce the benefit of RSVP.

Normally sighted younger and older (mean age 71 years) observers read sentences and random lists of words from a rapid serial visual presentation (RSVP) display and a scroll display using their normal vision and through two levels of cataract simulators. Unlike patients with central field loss (CFL), there was no decrease in the benefit of RSVP with reduced vision due to the cataract simulators. However, the usefulness of sentence-level context was reduced as visual acuity was reduced. In addition, older readers did not benefit as much from RSVP as younger readers, and many in the older group were unable to read using the more severe cataract simulators from either display format. From these data we conclude that the benefits of RSVP are not reduced with reduced acuity and contrast sensitivity, and that there are age-related changes in reading rates from dynamic text displays not related to acuity.

Adult↗
PubMed1997

Effects of imagery on vernier acuity under conditions of induced depth.

Imagery interferes with visual acuity (the "Perky effect") when an image is close to a visual target and both the image and the acuity target are located in the same depth plane. Whether imagery-induced interference occurs when a mental image and a target are separated by induced depth was investigated. Participants projects an image in front of or behind a vernier acuity target on a frontal or back plan suggested by the panels of an outline cube. A drop in accuracy for the target was found when an image was projected in front of, but not behind, the target. Thus, induced depth can influence the Perky effect. By contrast, real lines interfered with the target regardless of perceived depth plane, which is inconsistent with the hypothesis that imagery and perception are equivalent. Results support the hypothesis that images interfere with perception only when the participant must see through an image to obtain information specifying the visual target.

Depth Perception↗
PubMed1997

Transient lumanopia: the invisibility of flicker in early dark adaptation.

Flicker thresholds for brief white (5-arcmin, 16-18-Hz) foveal flicker bursts can rise for at least 1 s after a steady white adapting field is extinguished. We call this anomalous loss of sensitivity in early dark adaptation lumanopia by analogy with dyschromatopsia, a similar anomaly of the hue pathways. Lumanopia is greatest at 400 Td. Flicker thresholds for 10-12 Hz flicker bursts do not show lumanopia. The temporal-frequency dependence of lumanopia is specific to early dark adaptation, as flicker thresholds vary little from 10 to 18 Hz after steady light or dark adaptation. Lumanopia has implications for models of retinal gain controls.

Dark Adaptation↗