New method for the elution of platelet antibodies.
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Biomedical subjects
Publications and source records attributed to A Ribera.
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Following a total blood transfusion, a 65 year old woman suffered severe thrombocytopenia characterized by purpura and haemorrhage. An immunological study revealed the existence of IgG and IgM type antibodies in the serum attached to the patient's platelets and to the eluate obtained from these. The specificity of the antibody was Zwa. The patient's platelets are Zwa-negative. Even in the remission phase of the thrombocytopenia it was still possible to obtain antibodies in the platelets of the eluate. Questions raised by this factor are discussed and, despite the difficulties posed, an attempt is made to find a coherent immunological explanation based on the results of the serological study.
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The presence of antibodies against human immunodeficiency virus type 2 (HIV-2) was retrospectively investigated in serum samples from 433 parenteral drug abusers from Barcelona. A third generation ELISA was used for the initial screening of sera, and a specific western blot technique for the definitive confirmation of the results. The presence of anti-HIV-2 was demonstrated in 9 samples (prevalence 2.1%). All of them belonged to patients also infected with HIV-1. The 9 cases reported here are the first seropositive patients for HIV-2 described in native individuals from this country.
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We have studied retrospectively the presence of serologic markers of type 1 and type 2 human immunodeficiency virus infection in 102 subjects coming from West Africa and living in Catalonia. We have proven the presence of specific antibodies to type 2 human immunodeficiency virus in three asymptomatic subjects among whose epidemiologic antecedents the unique risk factor was heterosexual promiscuity. These three subjects are the first seropositive to type 2 human immunodeficiency virus reported in our country. In none of the studied cases serologic markers of type 1 human immunodeficiency virus infection were detected.
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