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Biomedical subjects

A Rotman

Publications and source records attributed to A Rotman.

At least 37 records · Page 2Linked to original sources
PubMed1980

Platelet uptake of serotonin in psychotic children.

Platelet serotonin (5-HT) uptake was assayed in ten long-term hospitalized children subdivided into two diagnostic groups "autistic" and "other psychotic". Weekly blood samples were taken from these children over a period of 6-7 weeks and serotonin uptake was determined. Constant and significant uptake values (13.28 +/- 2.0 pmol serotonin/10(8) platelets/5 min for autistics and 9.59 +/- 1.58 pmol serotonin/10(8) platelets/5 min for other psychotics) were obtained. There was no correlation with drug treatment or with behavioral fluctuations in individual children. Three weekly uptake assays performed on six parents suggest the possible importance of genetic factors in these diseases. Biochemical-kinetic methodological problems of platelet 5-HT assessment are discussed.

Adolescent↗
PubMed1980

A comparative uptake study of serotonin, dopamine, and norepinephrine by platelets of acute schizophrenic patients.

Active uptake of serotonin, dopamine, and norepinephrine by blood platelets of 22 acute schizophrenic patients and 15 normal control subjects was studied over a 6-week period. The Brief Psychiatric Rating Scale (BPRS) was used to evaluate the subjects' mental state over this period. Serotonin uptake by platelets of the schizophrenic group was 40% lower than that by platelets of the control group (p less than 0.001). No significant differences in uptake of dopamine or norepinephrine were observed. These results might reflected a genetic defect in the schizophrenic patients. No significant correlations were found between the biochemical and BPRS results. A correlation was found between the uptake results with dopamine and norepinephrine but not between these amines and serotonin. This finding may provide indirect support for previously published experimental data which suggest that the carriers to dopamine and norepinephrine differ in function from those of serotonin.

Adolescent↗
PubMed1979

Serotonin uptake by blood platelets of acute schizophrenic patients.

Active uptake of serotonin by blood platelets of acute schizophrenic patients has been compared to that of a control group. Preliminary results presented in this article indicate that the uptake of the schizophrenic patients was about 40% lower than that of controls. Patients were followed over a period of 5 weeks with no significant change in uptake.

Adolescent↗
PubMed1978

Nuclear magnetic resonance studies of 6-hydroxydopamine and its interactions with SH-containing model compounds. Evaluation of possible mechanism for neurocytotoxicity.

6-Hydroxydopamine (I) is a well-known neurocytotoxic agent which has become an important tool in many neurochemical studies in recent years. Biochemical investigations of the mechanism of action of 6-hydroxydopamine indicated that this amine binds covalently and irreversibly to proteins. In the present work, molecular properties of 6-hydroxydopamine in aqueous solution such as self-association, ionization, intramolecular conformations, and possible cyclization were investigated using 1H nuclear magnetic resonance spectroscopy. A model study for the interaction of 6-hydroxydopamine with proteins was undertaken by using SH-containing molecules: cysteine, glutathione, and bovine serum albumin. The binding of these compounds to 6-hydroxydopamine was found to cause labilization of the hydrogen attached to C2 of the amine aromatic ring. This effect was interpreted in terms of nucleophilic attack of RS- on C1 of 6-hydroxydopamine. A proposed model for neurocytotoxicity is discussed.

Cysteine↗
PubMed1975

Norepinephrine uptake sites in cardiac tissue. Lack of affinity of 6-hydroxynorepinephrine and related compounds.

The effects of a series of phenethylamines and the corresponding phenethanolamines on (i) rate of uptake of radioactive norepinephrine into cardiac tissue in vivo and (ii) the rate of efflux of radioactive norepinephrine from prelabeled cardiac storage sites have been determined. The results indicate that m- and p-hydroxuphenethylamines and the corresponding phenethanolamines have high affinities for uptake into cytoplasm and storage vesicles of noradrenergic terminals in the heart. o-Hydroxyphenethylamines such as 2-hydroxyphenethylamine and 2,4,5-trihydroxyphenethylamine (6-hydroxydopamine) also have moderate to high activity as inhibitors of norepinephrine uptake and as releasing agents for norepinephrine, but o-hydroxyphenethanolamines such as 2-hydroxyphenethanolamine, 2,5-dihydroxyphenethanolamine, and 2,4,5-trihydroxyphenethanolamine (6-hyproxynorepinephrine) have little or no activity as inhibitors of uptake or as releasing agents. 2,6-Dihydroxyphenethylamines have little or no activity as inhibitors of uptake or as releasing agents. The results are consonant with significant binding of the gauche conformers of 2-hydroxyphenethylamines to uptake sites. Such conformers would be preferred because of stabilization by hydrogen bonding between nitrogen and phenolic oxygen. Apparently a hydrophobic region of the site prevents binding of such stabilized gauche conformers of 2-hydroxyphenethanolamines and 2,6-dihydroxyphenetylamines.

Animals↗