[Cyclic nucleotides in malignant neoplasms of the colon].
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Biomedical subjects
Publications and source records attributed to A S Agabalian.
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The blood levels of cyclic nucleotides and serotonin were measured in 143 patients with functional and inflammatory diseases of the large intestine. The patients were divided into 3 groups administered various treatment: drug (basic) therapy in Group 1, basic therapy + alternating magnetic field in Group 2, and basic therapy + sinusoidal modulated currents in Group 3. Follow-up of the examined parameters over the course of treatment has shown that by day 16 of inpatient treatment they normalize, this demonstrating the treatment efficacy.
Plasmid DNAs were isolated from Bacillus thuringiensis and their physico-chemical properties were studied. The DNAs were purified using ultracentrifugation in a CsCl2 density gradient. The molecular weight of these extrachromosomal DNAs determined by electrophoresis in 0.6% agarose gel and by electron microscopy was 6 . 10(6)--10 . 10(6) daltons; a DNA with a molecular weight of 90 . 10(6) daltons was also found. Digestion with restriction endonuclease EcoRI yielded fragments of the plasmid DNAs with cohesive ends; these were further identified by means of electrophoresis in 1% agarose gel.
Physico-chemical characteristics of plasmid DNAs isoalted from Pseudomonas putida G7 were studied as well as the behavior of these DNAs in th eourse of chromatography on columns with Sepharose 4B and kieselguhr with methylated albumin (MAC). This strain was found to contain several plasmid DNAs having molecular weights of 33-36X10(6), 15-18X10(6), and 3-5X10(6) dalton. The plasmid DNAs of biodegradation are supposed to be located in the vicinity of chromosomes, and only a small part of them is characterized by extrachromosomal localization.