PROTEIN SYNTHESIS IN CELL-FREE PREPARATIONS FROM DROSOPHILA MELANOGASTER.
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Biomedical subjects
Publications and source records attributed to A S FOX.
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Embryonic cells isolated from eggs ofDrosophila melanogasterhave been cultured continuously in a new medium. Generation time for cell division is 30 hours. Chromosome number remains constant for at least 10 days. Cells from embryos of the mutant maroon-like grow at the same rate as those from wild-type embryos, but cells from rosy-2 grow slower and at a lower optimum temperature.
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All tested stocks of Drosophila melanogaster exhibit a peptide in males but not in females. The failure of some investigators to demonstrate its presence is attributable to their choice of chromatographic solvents. Acid hydrolysis confirms its peptide identity. Its presence in females transformed into males by the mutant tra implicates the sex-determining loci in its formation.
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