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Biomedical subjects

A S Kuznetsov

Publications and source records attributed to A S Kuznetsov.

86 records · Page 5Linked to original sources
PubMedDate not supplied

[Impairment of cholesterol-acceptor function of high density lipoproteins in patients with ischemic heart disease].

An ability of high density lipoproteins HDL3 to accept cholesterol from erythrocyte membranes was studied in healthy persons and in patients with ischemic disease of heart. The cholesterol-acceptory function was estimated as follows: by incorporation of fluorescent probes (cholestatriene and pyrene) into particles of HDL3, by elimination of cholesterol from erythrocyte membranes and by increase of the lipoproteins size evaluated using the method of quazi-resilient dispersion of laser light. In ischemic disease of heart the property of high density lipoproteins, specifically of HDL3 fraction, to accept cholesterol from cell membranes was impaired. Middle size of HDL3 particles was decreased in the patients with ischemic heart disease as compared with that of healthy persons.

Cholesterol↗
PubMedDate not supplied

[Interaction of plasma lipoproteins and apolipoproteins with lipid bilayer membranes].

It was shown that the interaction of lipoproteins (LP) with bilayer lipid membranes (BLM) resulted in some changes in the physical-chemical properties of the membranes. Adsorption of very low and low density lipoproteins (VLDL and LDL) at concentrations of 5-8 g protein/ml increased the surface potential difference and decreased transversal elasticity module of the bilayer. LP concentrations higher than the mentioned ones increased BLM conductance and caused instability and disruption of the membranes. The same effects were revealed for high density lipoproteins (HDL) at higher concentrations--15-20 micrograms protein/ml. The effect of apolipoproteins in the interaction of LP with BLM was investigated. It is proposed that apolipoproteins and especially apo B are the main factor which affects the nonreceptor interactions of LP with the membranes.

Adsorption↗
PubMedDate not supplied

[Interaction of low density lipoproteins with charged ligands: a study using the fluorescent probe 1-aniline naphthalene-8-sulfonate].

Heparin, chodroitin sulfates A, B, C, clofibrate and its derivatives as well as diphenyl disulfonate substituted the fluorescent probe I-aniline naphthalene-8-sulfonate in blood plasma low density lipoproteins. Constants of association for complexes of low density lipoproteins-heparin and chondroitin sulfate C, calculated on the basis of these data, were equal to 1.15.10(6) and 0,46.10(6) M-1, respectively. At the some time, the ligands studied altered the lipoprotein charges.

Chondroitin↗
PubMedDate not supplied

[Fluorescent probe--a cholesterol analog: localization in plasma lipoproteins and in model lipid particles].

The absorption spectrum of fluorescent probe--a cholesterol analog cholesta-5,7, 9(11)-trien-3 beta-ol has a vibrational structure with the maximum 326 nm. Its fluorescence spectra maximum is 370 nm. The localization of the probe in lipoproteins of high, low and very low density and in lipid spheres is studied. There are measured the areas, which occupied one molecule of cholesterol and phosphatidyl choline on the surface of lipid spheres and the radius of the lipid spheres. The localization of the probe in lipoproteins and lipid spheres is determined. The areas which occupied one molecule of phosphatidyl choline on the surface of lipid sphere is equal to the same area in mono- and bilayers. Cholesterol has the same condensing action on phosphatidyl choline in lipid spheres as in mono- and bilayers. All the probe molecules are localized on the surface of lipid spheres and lipoproteins and the B-ring of the molecule is immersed on 1.3 +/- 0.2 nm relative to polar groups. The hydroxyl group of cholesterol is arranged near the carbonyl group of phospholipid and the formation of the H-bond between these groups is possible.

Cholestenes↗
PubMedDate not supplied

[Determination of the radius, volume and surface area of plasma lipoproteins using fluorescent probes].

The effect of radiationless energy transfer between the fluorescent probes was used to determine the radius, volume and surface area of the blood plasma lipoprotein. Anthracene and p-terphenyl, distributed over the whole volume of lipids of a lipoprotein particle, and HSPH-14 localized on its surface served as energy donor and acceptor probes. Human blood lipoproteins of very low (LVLD), low (LLD2), and high (LHD2 and LHD3) density were studied. All the fluorescence-measured lipoprotein volumes were rather close to the data resulted from the weight analysis. The surface areas were equal to 230, 210, 400 and 330 m2 per 1 g of lipoprotein and the radii--11, 11, 7.5, 7.2 nm, respectively. All the measurements were made in solutions, without any denaturating effects on lipoprotein, its concentration being 1 mg/ml and lower.

Chemical Phenomena↗
PubMedDate not supplied

[Isoelectric focusing of high density lipoproteins in the wall of the human aorta].

Charge distribution of high density lipoproteins (HDL) from the human aorta intima has been studied using the method of isoelectric focusing in the polyacrylamide gel. Four groups of aortal HDL (A-HDL) were identified by focusing in the pH range 4.6-6.0: 1) the prevailing group (pH 4.7-4.9); 2) the intermediate, the most heterogeneous group (pI 5.0-5.9), including to 15-17 subfractions (these two groups are also present in the plasma); 3) the cathodic group (pI 6.0) practically absent in the plasma and one of the main groups among A-HDL (22% of all A-HDL), certain subfractions of this group have pI about 6.9; 4) the anodic group (pI 3.75), a minor group (5% of all A-HDL), also absent in the plasma. No differences are found between charge distribution of HDL from the normal aorta intima and atherosclerosis-affected intima. The data obtained confirm that plasmic HDL penetrating to the arterial intima are subjected to considerable changes in it.

Aged↗
PubMedDate not supplied

[The effect of blocking the reticuloendothelial system on the metabolism of low density lipoproteins in rabbits].

Intravenous ethyl oleate-induced blockade of the reticulo-endothelial apparatus (REA) in rabbits was found to reduce the elimination rate of both native and 125I-modified (acetylated) low lipoproteins. Lower lipoprotein catabolism is likely to result in hyperlipidemia observed in REA blockade. At the same time the latter was shown to induce the changes in low lipoproteins, which diminished their capacity to interact with fibroblasts, on the one hand, and to stimulate their macrophage uptake.

Animals↗
PubMedDate not supplied

[Genetic effect of incorporated 137 Cs in male mice after single injection of the isotope].

A comparative study of the incidence of genetic damages induced in mouse male germ cells by a single injection of 137Cs and external gamma-irradiation shows that the incidence of dominant lethal mutations in the postmeiotic cells is similar with both radiation types. The frequency of reciprocal translocations in stem cell spermatogonia was considerably lower with 137Cs than with external gamma-irradiation which might be attributed to the prolonged effect of the radionuclide.

Animals↗