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Biomedical subjects

A S Popov

Publications and source records attributed to A S Popov.

At least 19 recordsLinked to original sources
PubMed1989

[Creatine kinase isoenzymes in the diagnosis of acute cranio-cerebral trauma].

The cerebrospinal fluid (CSF) creatine kinase (CK) activity and isozymic spectrum (EC 2.7.3.2) have been examined in patients with craniocerebral injuries of varying severity. The CK activity has been elevated in all the patients. Three isoforms have been detected: CK-BB, CK-MB, and CK-MM. CK-BB has been detected in all the patients in the presence of the total CK activity; this is explained by the isozyme release from the brain tissue during the injury and as a result of functional and structural impairment of the cellular membranes in intensification of lipid peroxidation. The CK-MM activity is due to blood admixture in the CSF and to impaired hematoencephalic barrier during the injury. The presence of CK-MB in the CSF of patients without cardiac symptoms probably results from a recombination of CK-BB and CK-MM isoforms and is of no diagnostic significance. Measurements of the total and isozymic CK activity in the CSF of patients with craniocerebral injuries may become a test for the laboratory diagnosis of the trauma severity and course.

Adolescent↗
PubMed1989

[Creatine phosphokinase in the cerebrospinal fluid of patients with acute meningitis].

A study is presented of the general activity and isoenzymatic spectrum of creatine phosphokinase (CPK) in the cerebrospinal fluid (CSF) in patients with acute meningitis of different etiology. The enzymatic activity proved to be increased due to the cerebral (CPK-BB) isoform. Liberation of CPK-BB from the brain tissue and its penetration into the intercellular fluid and then into the CSF was related to the functional-structural changes in the cellular membranes of the brain in meningitis.

Acute Disease↗
PubMed1984

[Logical structure of the diagnostic process].

The main elements of the diagnosing process are analyzed from the phylosophical viewpoint. The due professional and phylosophical level of the physician is stressed to be necessary.

Diagnosis↗
PubMed1976

[Several methodologic features of prolonged human lymphocyte storage].

When using in the lymphocytotoxic reaction lymphocytes stored in frozen condition the proportion of perished cells after thawing should not exceed 10-20%. Therefore, a study was made of the influence of various concentrations of dimethylsulfoxide (DMSO), composition of the medium and of other parameters for the purpose of elaboration of the optimal conditions of freezing human lymphocytes. The best results were obtained in freezing cells in the autologous serum with 12.5% DMSO. However sufficiently favourable results were also seen in using AB serum and 20% bovine serum in Eagle's medium.

Blood Preservation↗
PubMed1976

[Dialectics of the objective and subjective in the diagnosis of diseases].

The reader's attention is drawn to the fact that the dialectics of the objective and subjective in the cognition process is one of the most important and "enternal" philosophic problems. At each historical step of development of the science and public practice, in each sphere or cognition activity the former is of a definite specificity. It is the authors' opinion that the same is fully refered to such complicated field of cognition as the diagnosis of diseases.

Diagnosis↗