PubMed Health⌕ Search

Biomedical subjects

A S Randhawa

Publications and source records attributed to A S Randhawa.

10 recordsLinked to original sources
PubMed1977

Agglutinins to Coxiella burnetii and Brucella spp, with particular reference to Brucella canis, in wild animals of southern Texas.

The prevalence of agglutinins to Coxiella burnetii and Brucella spp, particularly Brucella canis, was determined in 269 wild animals (14 species) in southern Texas. Serologic evidence of coxiellosis and brucellosis, including B canis infection, was shown for coyotes, raccoons, opossums, badgers, jackrabbits, and feral hogs. Using the microagglutination test, the seroprevalence of C burnetii, phases I and II (titer greater than or equal to 4) was 4.1 and 27.9%, respectively. For brucella agglutinins, prevalence rates were 7.1, 8.9, and 6.7%, as determined by the brucellosis card test, the rapid slide agglutination test, and the salt 2-mercaptoethanol tube agglutination (titer greater than or equal to 50) test, respectively.

Agglutinins↗
PubMed1977

Prevalence of seropositive reactions to Brucella canis in a limited survey of domestic cats.

The prevalence of Brucella canis agglutinins was determined in 170 cats (114 from animal shelters in California and 56 from an animal hospital in Texas). Seropositive reactions in the cats from animal shelters were 5.3, 11.4, and 0%, respectively, the the rapid slide agglutination test, salt 2-mercaptoethanol tube agglutination test, salt 2-mercaptoethanol tube agglutination test, and brucellosis card test. For hospitalized cats, the respective percentages were 7.1, 8.9, and 0%. One (0.9%) of 114 cats from the animal shelters and 5 (8.9%) of 56 hospitalized cats were seropositive by the salt 2-mercaptoethanol tube agglutination test at titers greater than or equal to 1:200. Isolation of bacteria was not attempted; thus, the findings of this study may need cautious interpretation.

Agglutination Tests↗
PubMed1977

Variables affecting viral plaque formation in microculture plaque assays using homologous antibody in a liquid overlay.

A liquid antibody microculture plaque assay and the variables that govern its effectiveness are described. The assay is based on the principle that low concentrations of homologous antibody can inhibit secondary plaque formation without inhibiting formation of primary plaques. Thus, clear plaques that followed a linear dose response were produced. The assay was found to be more rapid, less cumbersome, and less expensive than assays using agar overlays and larger tissue culture plates. It was reproducible, quantitative, and had about the same sensitivity as the agar overlay technique in measuring infectious coxsackievirus type B-3. It was more sensitive in assaying adenovirus type 3 and Western equine encephalomyelitis, vesicular stomatitis, Semliki forest, Sendai, Sindbis, and Newcastle disease viruses than were liquid, carboxymethylcellulose, and methylcellulose microculture plaque assays. The variables influencing sensitivity and accuracy, as determined by using coxsackievirus type B-3, were: (i) the inoculum volume of virus; (ii) the incubation period of virus; and (iii) the incubation temperature.

Adenoviruses, Human↗