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A S Skopelitou

Publications and source records attributed to A S Skopelitou.

6 recordsLinked to original sources
PubMed2001

Heterotopic gastric mucosa together with intestinal metaplasia and moderate dysplasia in the gall bladder: report of two clinically unusual cases with literature review.

We report the clinicopathological findings of two patients with ectopic gastric mucosa within the gall ladder. The first patient, a 78 year old man, was asymptomatic. He was admitted to hospital for a colon adenocarcinoma. Intraoperatively, a firm nodule was palpable in the gall bladder. Histological examination of the resected specimen revealed a body type gastric mucosa in the submucosa, adjacent to which were extensive pyloric gland and intestinal metaplasia with mild to moderate dysplasia. The remaining gall bladder mucosa demonstrated changes of chronic cholecystitis. The second patient was a 62 year old woman with symptoms of chronic cholecystitis. The preoperative diagnosis was consistent with this diagnosis with a "polyp" at the junction of the neck and cystic duct. Cholecystectomy was performed and the histological examination of the resected specimen showed that the "polyp" consisted of heterotopic gastric mucosa with glands of body and fundus type. In the remaining mucosa, chronic cholecystitis was evident. To the best of our knowledge, this is the first report of a clinicopathological presentation of heterotopic gastric mucosa, pyloric gland type, and intestinal metaplasia with dysplastic changes in the gall bladder. As heterotopic tissue may promote carcinogenesis of the gall bladder, close attention should be paid to any occurrence of such lesions in this anatomical region.

Aged↗
PubMed1997

Effect of interferon-alpha2b administration on rat liver regeneration after partial hepatectomy.

The purpose of the present study was to delineate the effect of interferon-alpha2b (IFN-alpha2b) administration on the liver regenerative capacity after partial hepatectomy in rats. The administration of IFN-alpha2b simultaneously with partial hepatectomy did not affect hepatic proliferation in a statistically significant manner. When IFN-alpha2b was administered either 2 or 12 hr postoperatively, an inhibition of hepatocyte proliferation was observed 24 hr postoperatively, while at further time intervals up to 48 hr, DNA synthesis remained similar to that observed in the simply partially hepatectomized rats. The enzyme thymidine kinase (TK), has been implicated in the suppression of proliferation in interferon-treated cell cultures. In all IFN-alpha2b-treated groups of rats, alterations of TK activity were observed without being correlated to the liver regenerative status. Additionally, the administration of the polyamine putrescine in partially hepatectomized rats treated at the time of surgery with IFN strongly enhanced TK activity, but did not affect DNA biosynthesis. In the above-mentioned in vivo model of controlled cellular proliferation, the administration of IFN-alpha2b affected the rate of hepatocyte proliferation depending on the time of its administration; this effect was not correlated to the enzymatic activity of TK, as inhibited TK activity is responsible for the suppressed DNA synthesis in in vitro systems.

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PubMed1994

Proliferating cell nuclear antigen (PCNA) expression in regenerating rat liver after partial hepatectomy.

Proliferating cell nuclear antigen (PCNA) is a nuclear protein maximally elevated in the S phase of proliferating and transformed cells and is recognized by the monoclonal antibody PC-10 in paraffin tissue sections. The liver regenerative process after partial hepatectomy in rats was estimated with the in vivo incorporation of [3H]thymidine into liver DNA and the liver thymidine kinase activity. The expression of PCNA in rat liver after partial hepatectomy was performed by immunohistochemical staining with PC-10 in paraffin embedded tissues, at different time intervals up to 240 hr. Proliferating cell nuclear antigen expression, [3H]thymidine incorporation into DNA, and liver thymidine kinase activity exhibited marked oscillations during the liver regenerative process. A close relationship was demonstrated among DNA synthesis, thymidine kinase activity, and PC-10 score. Our results suggest that PC-10 monoclonal antibody may be used as a worthwhile proliferation index in the evaluation of the rate of liver regeneration in rats.

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PubMedDate not supplied

FHIT gene expression in human urinary bladder transitional cell carcinomas.

BACKGROUND: Our purpose was to investigate the expression of FHIT (Fragile Histidine Triad) gene product in a series of 110 urinary bladder TCCs, and its eventual relationship with histological grade, clinical stage, recurrences and patients' survival. MATERIALS AND METHODS: We performed immunohistochemistry in archival material of formalin-fixed, paraffin-embedded tissues, using the anti-Fhit antibody and the Streptavidin-biotin peroxidase method. RESULTS: In 30 out of 110 cases (27.27%) Fhit protein was absent whereas in 32 cases (29.08%) it was abnormally expressed. In 48 cases (43.63%) Fhit protein was diffusely expressed in all tumor cells. A statistically highly significant correlation (p < 0.001) was noticed between Fhit protein absence or reduction and clinically advanced tumors. Conversely, abnormal Fhit protein expression was not associated with age, histological grade, tumor size, number of recurrences, and clinical outcome in terms of patients' survival. CONCLUSIONS: These results confirm that FHIT gene inactivation is a late event in urinary bladder carcinogenesis. Fhit protein reduced expression or complete absence correlates with advanced clinical stage of the disease, and does not seem to correlate with tumor recurrences and patient survival.

Acid Anhydride Hydrolases↗