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Biomedical subjects

A S Stern

Publications and source records attributed to A S Stern.

59 records · Page 4Linked to original sources
PubMed1980

Enkephalin biosynthetic pathway: proteins of 8000 and 14,000 daltons in bovine adrenal medulla.

Two [Met]enkephalin-containing polypeptides have been purified from extracts of bovine and adrenal chromaffin granules. One is 8000 daltons in size and contains the enkephalin sequence at the carboxy terminus. The other (14,000 daltons) has two internal enkephalin sequences and a third sequence at the carboxy terminus. All of these sequences can be released by trypsin, indicating a possible enkephalin precursor role for the polypeptides.

Adrenal Medulla↗
PubMed1980

Analgesic activity of the naturally occurring heptapeptide [Met]enkephalin-Arg6-Phe7.

[Met]Enkephalin-Arg6-Phe7 is an opiate-like peptide normally found in the the adrenal gland and brain that has analgesic (antinociceptive) activity when administered directly into the cerebral ventricles of mice. On a molar basis, [Met]-enkephalin-Arg6-Phe7, with a median effective dose (ED50) of 38.5 nmol/mouse, is 8 times more potent than [Met]enkephalin. As with [Met]enkephalin, analgesic activity is blocked by naloxone and intravenous administration does not produce characteristic opiate effects in tests for analgesic, antidiuretic, or antidiarrheal activity. These findings suggest that [Met]enkephalin-Arg6-Phe7 may be at least as important as the enkephalins in the postulated enkephalin system mediating pain and analgesia.

Analgesics↗
PubMed1979

Isolation of the opioid heptapeptide Met-enkephalin [Arg6,Phe7] from bovine adrenal medullary granules and striatum.

Bovine adrenal chromaffin granules have been shown to contain, in addition to Met-enkephalin and Leu-enkephalin, at least three small peptides with opiate receptor activity. One of these adrenal peptides has been purified to homogeneity and its sequence was shown to be Met-enkephalin-[Arg6,Phe&]. This heptapeptide was also found in beef striatal extracts in amounts comparable to those of Leu-enkephalin.

Adrenal Medulla↗