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Biomedical subjects

A S Watson

Publications and source records attributed to A S Watson.

17 recordsLinked to original sources
PubMed1995

Molecular evolution of the F glycoprotein of human parainfluenza virus type 1.

Human parainfluenza virus type 1 (hPIV1) is a major cause of upper and lower respiratory tract infections among children. Immunity is mediated at least in part by antibody to the fusion (F) surface glycoprotein. Thus, genetic variation in the F gene could influence host range, virulence, and immunity. To examine the genetic diversity among hPIV1 isolates, the F genes of hPIV1 isolates from a single geographic location were sequenced and compared with the F gene of a strain isolated in 1957. Genetic variation was 2.2%-3.4%, averaging 0.8 amino acid changes per year. Changes were progressive over time, and virus evolution was dominated by a single lineage. Three of 7 isolates tested did not induce syncytium formation in tissue culture. This phenotype could not be ascribed to a single unique mutation in the F gene, but these 3 isolates had mutations in the transmembrane region of the HN gene. It is unlikely that the limited genetic evolution of the F gene will be an obstacle to vaccine development.

Amino Acid Sequence↗
PubMed1995

Sequence and analysis of the rpoB gene of Mycobacterium smegmatis.

The rpoB gene encodes the beta subunit of the DNA-dependent RNA polymerase of bacteria. Mutations in defined areas result in resistance to rifampin. Mycobacterium smegmatis is naturally resistant to rifampin, but analysis of the rpoB gene revealed no identifiable rifampin resistance mutations. Another mechanism of resistance may be present.

Amino Acid Sequence↗
PubMed1995

Severity of alcohol dependence in the East End of Glasgow.

A survey of 100 consecutive admissions for alcohol abuse in 1990 highlights the severity of alcohol dependence in the East End of Glasgow. The survey identifies a specific sub-group of heavy drinkers who consume strong lager, usually in open spaces, referred to locally as 'garden parties'. In the total population, males outnumbered females 3:1 with a mean consumption of 268 and 230 units of alcohol per week, respectively. The severity of alcohol abuse was reflected in their social, physical and central nervous system complications. Two-thirds of the population had a family history of alcohol abuse. Over three-quarters of the population showed self neglect with nearly half the males ascribing their marital breakdown or family neglect directly to their drinking problem. Both sexes showed a high prevalence of history of paraesthesiae, while half the male population had clear signs of peripheral neuropathy on admission. A history of delirium tremens in over 50% of the population with a history of seizures in 21% of males and 35% of females indicates central nervous system involvement. Three sub-groups were identified: history of delirium tremens, history of persecutory ideation and history of serious criminality. All three sub-groups had a shorter length of drinking with higher consumption and increased frequency of 'garden parties' than the group as a whole. This survey reinforces the need for appropriate in-patient detoxification and rehabilitation facilities for patients with severe alcohol dependence, to reduce their impact on other health, social work and criminal justice services.

Alcohol Drinking↗
PubMed1994

The translation efficiency of ovalbumin mRNA is determined in part by a 5'-end hairpin structure.

We have investigated the role in translation of the secondary structure in the noncoding leader (NCL) sequence of ovalbumin mRNA. Deletion of a stem-loop structure from the mRNA 5'-end (hairpin-1) produced a 2.5-fold decrease in mRNA translation rate in both rabbit reticulocyte wheat germ cell-free systems. A corresponding 2-fold reduction in mRNA binding affinity for reticulocyte eucaryotic initiation factor 2 (eIF-2) was also observed. These effects were independent of mRNA capping. Both translation rate and eIF-2 binding affinity were restored by addition to the mRNA of a sequence containing a hairpin-1-like structure. The positive correlation between cell free translation rate and mRNA binding to eIF-2 suggests that hairpin-1 is both an initiation signal and part of a specific eIF-2 recognition site. Methylation interference indicated a direct interaction between eIF-2 and hairpin-1 of ovalbumin mRNA.

Animals↗
PubMed1994

Human parainfluenza virus type 1 evolution combines cocirculation of strains and development of geographically restricted lineages.

The hemagglutinin neuraminidase (HN) glycoprotein of human parainfluenza virus type 1 (HPIV-1) mediates attachment to the host cell and is the target of protective antibody. Since the efficacy of a potential vaccine depends on antigenic constancy, the antigenic and genetic stability of the HPIV-1 HN glycoprotein was examined for 13 isolates obtained between 1981 and 1989. Antigenic analysis with a panel of 11 monoclonal antibodies demonstrated a single change among 3 isolates from 1989 that distinguished them from all other isolates. The HN genes from all 13 isolates and 13 previously published HN gene sequences shared > 95% homology. Evolutionary analysis demonstrated cocirculation of strains, without a dominant lineage. The 1989 isolates and the previously proposed subtype A isolates occupied distinct evolutionary branches, indicating geographically limited evolution. The slow rate of evolution and HN homogeneity may allow development of a single vaccine formulation for the prevention of disease.

Antigens, Viral↗
PubMed1992

The evolution of legal methods for dealing with mind-state in crimes.

From its very beginnings, British and American common law have attempted to deal with the mind-state of perpetrators of crime. This has taken various procedural and statutory forms that have often been characterized by confusion and contradictions in purpose. Clarification of the psychological and legal issues may assist in bringing the criminal treatment process to a more rational basis.

Expert Testimony↗
PubMed1988

Jumper's knee.

Explore the source record for details and available documents.

Athletic Injuries↗
PubMed1981

Cysts and "pseudocysts" of the menisci of the knee joint.

A series of 636 consecutive knee arthrotomies was subjected to a prospective study, including the incidence, clinical presentation, and operative findings of meniscal cysts and associated pathological conditions. The clinical differential diagnosis was studied in detail, and from this, the importance of a specific clinical sign indicating a torn lateral meniscus was realized. This sign was termed a "pseudocyst". It was concluded that meniscectomy was the operation of choice and not simple cyst removal, and that the high incidence of associated meniscal tears and other less frequent joint pathological conditions justified a standardized approach designed to assess the meniscus and other intraarticular structures, rather than a simple incision over the joint-line swelling.

Adult↗
PubMedDate not supplied

The meniscal "pseudocyst." A clinical sign of a torn meniscus.

We report a study of 636 patients requiring knee surgery, all of whom underwent detailed preoperative assessment. Fifty-eight patients had a clinical sign of a lump on the joint line when the knee was examined at 45 degrees of flexion, which has been thought to indicate a meniscal cyst. Of these 58 patients, however, only 30 patients had a meniscal cyst demonstrated at surgery. The remaining 28 patients had a meniscal tear without a cyst. In these 28 cases, the clinical sign of a lump protruding from the joint line was termed a "pseudocyst." This new clinical sign is important because of its frequency of occurrence and the complete correlation with meniscal tears requiring surgical intervention.

Adult↗