PubMed Health⌕ Search

Biomedical subjects

A Sárváry

Publications and source records attributed to A Sárváry.

16 recordsLinked to original sources
PubMed2001

Factor XIII of blood coagulation as a nuclear crosslinking enzyme.

Intracellular localization and distribution of Factor XIII subunit A (FXIIIA) was investigated in association with monocyte-macrophage differentiation in a long term culture of human monocytes by light- and electron microscopical as well as biochemical and immunobiochemical techniques. To allow the detection of FXIIIA in cells with well-preserved ultrustructure, immunosera against glutaraldehyde-derivatized recombinant FXIIIA were developed in rabbits, then characterized and used in this study. In the early phase of macrophage differentiation intranuclear accumulation of FXIIIA was detected as a transient phenomenon in cells of the 2nd day culture by optical sectioning with 0,7 microm steps in laser scanning confocal microscopy and immunoblotting technique. FXIIIA could be detected by immunoelectron microscopic postembedding staining over electrodense DNA-containing areas. Fluoresceinated monodansylcadaverine incorporation assay was used to demonstrate that FXIIIA is not only present in the nuclei, but also expresses its transglutaminase activity. Our finding of the nuclear accumulation of FXIIIA in differentiating human macrophages is also unique in that a blood clotting factor has, for the first time, been localized in nuclei and has been shown to be an intracellular crosslinking enzyme. The possible role of nuclear FXIIIA in association with cellular processes involving chromatin structure remodeling, such as cell death, cell differentiation or cellular proliferation requires further in-depth investigation.

Cell Culture Techniques↗
PubMed2000

Effect of tumor-conditioned medium on intercellular communication and proliferation of Balb/c 3T3 cells.

The possible role of tumor cell-derived factors in the regulation of gap junctional intercellular communication and proliferation of fibroblasts was studied in a model system of Balb/c 3T3 cells growing in tumor conditioned medium by Lucifer Yellow CH dye-transfer and BrdU incorporation assays. Six to 24 h incubation of Balb/c 3T3 cells in a medium conditioned by WiDr adenocarcinoma cells enhanced the gap junctional communication between the cells by 25-40% as revealed by intercellular transfer of a fluorescent dye Lucifer Yellow CH. Simultaneously the cell proliferation rates were examined and found to be reduced by 23% at 24 h treatment. Since adenocarcinoma cells are known to secrete different growth factor-like polypeptides into their conditioned medium, we suppose that tumors that produce these molecules might alter their host environment through the enhancement of cell-cell communication thereby facilitating the exchange of modulatory factors.

3T3 Cells↗
PubMed1998

Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy.

Besides flow cytometry, fluorescence microscopy combined with computerized image analysis offers an alternative tool for assessing phagocyte oxidant generation at the single-cell level. This technique provides an opportunity for the direct visualization of cells and simultaneous measurement of cellular fluorescence intensity. Thus, we developed a simple method for the quantitative evaluation of intracellular superoxide anion and hydrogen peroxide production with image cytometry by using hydroethidine and dihydrorhodamine 123 dyes, respectively. Human neutrophils stimulated with phorbol dibutyrate and labeled by these fluorogenic substrates showed intense, well recognizable red or green fluorescence. The intensity of signals from individual granulocytes of cytospin preparations were quantitatively measured in digitized images. There was a great heterogeneity in response to the stimulus within the granulocyte population as shown by the integrated fluorescence intensity values. In agreement with the results of parallel flow cytometric experiments, this simple image analysis performed on cells of cytospin preparations was able to detect the defects in the oxidative metabolism of neutrophils from patients with cervix carcinoma. We demonstrated that even minor alterations in superoxide anion/hydrogen peroxide generation can be detected by image cytometry as efficiently as by flow cytometry. This result validates imaging microscopy as an alternative to flow cytometry in such experiments. In addition, the image cytometric technique allows the observation of the kinetics of free radical production in individual cell under adherent conditions. Therefore, we carried out image analysis of the oxidative burst of neutrophils adherent to uncoated glass and fibronectin- and type IV collagen-coated surfaces in response to stimulation with phorbol dibutyrate or N-formyl-methionyl-leucyl-phenylalanine. We elaborated a calibration technique for the quantitative measurement of the ethidium bromide generation mediated by superoxide anion within individual adherent granulocytes. The ethidium bromide production varied between 0.48 and 1.17 amol/cell/min.

Anions↗
PubMed1986

[Claw plates--new implants for para-articular osteosynthesis].

The claw plate types used by us are manufactured according to the same principle. These plates are mostly applied in the treatment of multiple fragment fractures and in the fixation of osteotomies. A strong fixation of the plate, also to little fragments close to the joint, is achieved by the claws and one screw. The plates may be used for neutralization or as a support, distance piece, or compression plate. The shape of the variable plate as well as the claws can be formed. The masses of the maximum and minimum plate are adapted to the different sizes of bones. No special instrument is needed to insert the claw plate, and the region around the joint can be laid open in a sparing manner. Regarding the experience accumulated during ten years and the good results obtained, we are of the opinion that claw plates can be used successfully to complete the methods applied hitherto in the treatment of joint-near fractures of all long bones except the femur.

Ankle Joint↗
PubMed1984

[The role of the fibula in the stability of the lower leg].

The authors wanted to study on 24 prepared lower leg sceleton the biomechanical role of the fibula in a "total load" position. The supporting capacity of the fibula makes nearly 1/3 of the total, therefore the surgical reconstruction of the fibula can be useful in the treatment of fractures of the lower leg.

Biomechanical Phenomena↗
PubMed1981

[A new way of producing autogenous cancellous bone graft (author's transl)].

Transplantation of autogenous cancellous bone graft is used with an increasing frequency in the operative treatment of fractures or in reconstructive procedures for stimulating bone healing, replacing traumatic or other defects, treating non-unions, especially in septic conditions. The amount of autogenous cancellous bone available for transplantation often is below the requirements in certain cases, e.g. in large bone defects or when repeated transplantations have to be done. To cover these needs, the author filled the donor site in the iliac crest with large amount of "specially treated" (disantigenized) calf cancellous bone. 10 weeks later a known quantity of fibrous reticular tissue with osteo-inductive capacity filled the framework of the implanted graft, which could be transplanted as autogenous cancellous bone. The procedure can be repeated according to further requirements.

Animals↗
PubMed1978

[The use of a new hook plate for the operate treatment of ankle fractures (author's transl)].

Authors describe their methods of treatment and results in 137 inpatients with ankle fractures treated during a period of one and half year. Analysing this material the indications and methods of surgical treatment for ankle fractures are dealt with. In order to facilitate the surgical treatment of the most common type of ankle fractures (i.e. pronation fractures in group B of Weber) authors constructed a hook plate, adapted to the external malleolus, which proved good in their practice. Within this material it was used for 38 patients. 77 per cent of the displaced ankle fractures were treated surgically. After an average of 13.8 months follow up time the results were found satisfactory, using an evaluation system adequat for international comparison. With their data the efficiency of the surgical treatment for displaced ankle fractures is emphasized. The new hook plate is first of all used for the operative treatment of pronation fractures in level of the syndesmosis, and is recommended on basis of good experiences.

Ankle↗