Biomedical subjects
Publications and source records attributed to A Sattar.
The study of the first 1,000 CT examinations performed in Taiz (Yemen Arab Republic: YAR) reveals that 57.6% of these examinations have an abnormal CT appearance. The traumatic and tumoral pathologies predominate while the parasitic diseases are less. The results suggest some medical priorities for the YAR.
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Joint tissues from patients with rheumatoid arthritis (3), osteoarthritis (3) and normal healthy individuals (2) were examined in the transmission electron microscope and the morphological features of capillaries within 2 mm of the synovial membrane were quantified. Weibel-Palade bodies (WPB) were significantly increased in capillary endothelial cells in rheumatoid and osteoarthritis compared with normal controls, when quantified by three different methods. WPB were positively correlated with both projections of the luminal plasmalemma and pinocytic vesicles in normal joint and some patients with rheumatoid and osteoarthritis. Pinocytic vesicles and projections were also positively correlated.
A rapid and simple procedure for the direct solubilization of DNA from adherent cultured cells, or sedimented cells, has been developed and employed in the measurement of DNA in small numbers of endothelial cells (1 X 10(3).
Acetylated low density lipoprotein (Ac-LDL) is taken up by bovine aortic and adrenal capillary cells but not by brain capillary cells. This indicates that the uptake of Ac-LDL is not a feature of all types of endothelial cell. A quantitative examination of lipoprotein uptake by flow cytometry showed cells in G2M took up 45% more Ac-LDL than those in G1S. Compared with confluent cultures, sub-confluent bovine aortic cells took up three times as much LDL but Ac-LDL uptake was increased by only 34%. This indicates that the Ac-LDL receptor is not down-regulated to the same extent as that for LDL.
An analysis was made of ultrastructural changes in capillary endothelial cells in experimentally induced angiogenesis and in a human pathological situation known to involve increased angiogenesis. Chick chorioalloantoic membrane (CAM) showing a positive angiogenic response to low-molecular weight angiogenesis factor isolated from rat Walker sarcoma or from human rheumatoid joint was compared with untreated CAM. Serotonin-treated CAM provided an additional control in that serotonin has the capacity to stimulate endothelial cell growth in vitro but did not induce angiogenesis on the CAM. Human rheumatoid joints were studied using normal healthy human joints as controls. The number of Weibel-Palade (W-P) bodies per unit of cytoplasmic area were higher in tumor angiogenesis factor-treated CAMs (not significant) and rheumatoid angiogenesis factor-treated CAMs (P less than 0.008) than in untreated controls. These differences were more pronounced if W-P body volumetric density was analyzed (P in both cases less than 0.008). Serotonin-treated control CAMs did not show higher numbers of W-P body or greater WPV than untreated controls. Numbers of W-P body and W-P body volumetric density were higher (P less than 0.008) in rheumatoid joints than normal joints. Median values for W-P body number were 16-fold higher and, for W-P body volumetric density, they were up to 30-fold higher in rheumatoid joints.
Aspergillus tamarii Kita. isolated from seeds of Paspalum scrobiculatum L. is found to produce ergot alkaloids in submerged culture. The culture filtrate and mycelium are observed to contain 0.125 mg/mL and 1.2 mg/g (dry weight) total alkaloids consisting of 86.5 and 91.3% fumigaclavine A, respectively. The identification of the compound was confirmed by high-performance liquid chromatography, ultraviolet, infrared, and mass spectrophotometry analyses. This is the first report of the production of ergot alkaloid by this fungus. The possible role of fumigaclavine A as a mycotoxin is discussed.
Sulfur-containing steroids, analogs, and derivatives were synthesized for evaluation in mice suffering acute toxicity from methylmercuric chloride. Steroids were administered by intraperitoneal injection, by stomach tube feeding, or by absorption through the tail skin. Thiocholesterol and the thiocholanoic acids were effective if given prior to poisoning. The thiosteroids were significantly more effective than penicillamine or dimercaprol under these conditions.
Dibucaine Number (DN) was determined in 103 patients suffering from duodenal ulcer diagnosed radiologically and 214 normal subjects (control). Statistically significant (p less than 0.001) difference was observed in the distribution of DN in the two groups. Whereas 94% of the control subjects had normal DN (above 70), 6% had intermediate DN (between 40--70) and none had atypical DN, only 32% of patients suffering from duodenal ulcer had normal DN, 64% had intermediate DN and 4% had atypical DN. The results have been discussed.
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1. Spectrophotometric determination of ampicillin showed comparable results with microbiological assay. 2. A bioavailability study with five brands of ampicillin revealed variations in the serum concentration at 3 hr. but showed somewhat similar concentrations at 6 hr. The results have been discussed.
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Explore the source record for details and available documents.
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In this article, we initially review several problems associated with the design and interpretation of certain types of experiments currently used to study wound healing, drawing attention to the fact that applications for standard statistical techniques in the analysis of the experimental results are often of limited value. We then argue that, because of the special nature of wound healing data, curve fitting of empirical model equations can often provide a convenient way to summarize treatment effects with large data sets. The various ways in which this technique could be used to facilitate the interpretation of experimental wound healing results are then explored. To illustrate this approach, we then took several wound healing experiments and introduced possible models that could be used, paying particular attention to simple equations with the smallest possible number of parameters. For each equation, the way that the parameters of the model could be interpreted with regard to the biologic effects represented is given. Examples are given to show the application of each model discussed theoretically in the interpretation of some typical experimental data sets.
BACKGROUND: Presence of dyslipidemia, i.e. raised Total Cholesterol (TC) and decreased High Density Lipoproteins (HDL) is an established phenomenon in type 2 diabetes mellitus. Its relationship to the fasting blood sugar (FBG) level in both diabetics and non-diabetics has yet to be established. The present study aims to show such a relationship. METHODS: 150 subjects were selected and divided into two groups, one of 50 controls (non-diabetics), the other of 100 patients with type 2 diabetes mellitus. Their FBG levels were determined by enzyme oxidase method, and their TC and HDL levels were determined using a standard kit method. RESULTS: FBG and TC:HDL ratios were increased in the patient group (p = < 0.001). Comparison of FBG and TC:HDL ratios revealed a highly significant rise (p = < 0.001) in the patient group. FBG with TC:HDL ratios between both groups showed a positive correlation (r = 0.554). CONCLUSION: The results of the present study suggest an association between FBG and TC:HDL ratios, which may be a contributory factor to the increased prevalence of coronary artery disease in patients with type 2 diabetes mellitus.