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Biomedical subjects

A Sawaguchi

Publications and source records attributed to A Sawaguchi.

8 recordsLinked to original sources
PubMed1992

Medicolegal diagnosis of asphyxia--cerebral blood flow and brain oxygen tension.

Over the last several years, using rabbit models the authors analyzed cerebral blood flow (CBF), the brain oxygen tension (PtO2) and other physiological parameters during asphyxiation as means of achieving a practical diagnosis of asphyxiation. The author reports here on the effects of compression of the carotid arteries and obstruction of the airway leading to hypoxia, based on an analysis of CBF and PtO2 during hanging. Immediately after hanging, the CBF dropped but then rose suddenly. After several minutes, it dropped along with blood pressure. The PtO2 continued to decrease until the time of irreversible apnea. These findings suggest the effects of compression of the carotid arteries and the airway occlusion. After death, these results were confirmed with the injection-molded resin specimens of the airway and by the angiograph. But the closure of the vertebral arteries was not produced by the hanging.

Animals↗
PubMed1992

Re-evaluation of death by asphyxia based on cerebral blood flow (VII)--on the information on electric potentials obtained from deep electroencephalographic electrodes.

The author presents electroencephalographic patterns (a physiological parameter of the brain function observed at rapid and fatal drowning of rabbits), cerebral blood flow (CBF) which support the physiological activities of the brain, and the relationship between systemic respiration and circulation with special reference to changes in the electrical potentials detected by deep electroencephalographic electrodes. The possibility of diagnosis of fatal drowning is examined, based on the results.

Action Potentials↗
PubMed1991

Allele frequencies of human complement factor I in a sample from Iwate, northern Japan, with the description of geographical cline.

Serum samples from 270 healthy blood donors of Iwate prefecture, northern Japan, were examined for polymorphism of factor I (IF) by using polyacrylamide gel isoelectric focusing followed by semidry horizontal electroblotting with enzyme immunoassay. In 270 individuals four different patterns were observed, and these were controlled by two common alleles, IF*A and IF*B, and one rare allele, IF*A2. Allele frequencies were estimated to be 0.1019, 0.8963 and 0.0018 for IF*A, IF*B and IF*A2, respectively. The data of IF allele frequencies thus far reported in Japan excluding Okinawa Island were compared, and a statistically significant (p less than 0.01) geographical cline was detected for IF*A and IF*B alleles.

Alleles↗
PubMed1990

Genetic polymorphism of human factor H (beta 1H globulin).

Polyacrylamide gel isoelectric focusing (PAGIEF) of EDTA plasma and neuraminidase-treated plasma samples at pH 3.5-9.5 containing 8.0 M urea followed by an electroblotting with enzyme immunoassay was applied for the detection of factor H (HF) phenotypes in 536 unrelated Japanese blood donors living in Tokyo. In the major cathodal components, phenotypes of HF were classified into three common and five rare patterns, and these were considered to be controlled by two common and two rare alleles. The data suggest that the HF*Q0 allele also exists in the Japanese population. Family studies confirm the hypothesis that the HF polymorphism is controlled by autosomal codominant Mendelian inheritance.

Complement C3b Inactivator Proteins↗
PubMed1990

Factor I reference typing report.

Complement factor I (IF) reference typing has been examined using polyacrylamide gel isoelectric focusing followed by electroblotting with enzyme immunoassay. The submitted samples could be classified into three common and two new variants, and these were controlled by two common and two rare alleles. Two common alleles were IF*B (the most common allele) and IF*A, and two new rare alleles were designated IF*A1 and IF*B1, respectively.

Alleles↗
PubMed1990

Cerebral blood flow and serum haptoglobin in the diagnosis of death by rapid drowning.

Diagnosis of death and the feasibility of resuscitation in cases of acute drowning in fresh water (FW) or sea water (SW) inhalation in rabbits were evaluated using cardiopulmonary function monitoring, measurement of cerebral blood flow (CBF) and determination of haptoglobin (HP). Death took place more quickly in FW drowning, two to five minutes, as compared with four to seven minutes required in SW drowning. In view of the CBF patterns in the brain circulation, many problems exist regarding the judgement of the point of death and the applicability of resuscitation. However, it is considered that brain damage in SW drowning occurs more rapidly than in FW drowning.

Animals↗
PubMedDate not supplied

Factor I (IF) polymorphism in the Japanese population: existence of a new variant and quantitative analysis of different allotypes.

Polyacrylamide gel isoelectric focusing (PAGIEF) of neuraminidase treated EDTA plasma samples at pH 7-9 with 1.0 M urea followed by semi-dry horizontal electroblotting with enzyme immunoassay was done for the detection of factor I (IF) allotypes in 604 unrelated Japanese blood donors living in Tokyo. In these samples three common types, IF A, IF AB, and IF B, and a new variant were observed. A new variant which had major bands in a more acidic region than IF A was designated IF A1. Family data was in accordance with the Mendelian inheritance. The allele frequencies were estimated as IF*B = 0.8924, IF*A = 0.1068 and IF*A1 = 0.0008, respectively. The IF protein level of various allotypes was measured using the single radial immunodiffusion technique. The mean IF protein level for IF B was slightly higher than that for IF A (P less than 0.001) and that for IF A1B (P less than 0.05).

Alleles↗