Gluconeogenesis and palmitate incorporation into triacylglycerols and phosphatidylcholine were measured in isolated hepatocytes from control and ethanol-treated rats. Basal gluconeogenesis and its hormonal response decreased in hepatocytes from ethanol-treated animals; palmitate incorporation into triacylglycerols increased. In ethanol-treated rat liver, 45Ca2+ uptake and methylating capacity were reduced, and the hormonal response exhibited differences in binding parameters of insulin.

Animals↗

PubMedDate not supplied

Acetylated low-density lipoproteins promote the release and metabolism of arachidonic acid by murine macrophages.

We studied the effect of acetyl-LDL on the synthesis of eicosanoids by primary cultures of mouse-resident peritoneal macrophages. A dosis of 150 micrograms of acetyl-LDL proteins promotes a maximal release of arachidonic acid metabolites into extracellular medium. This process is very rapid and reaches the maximal value about ten minutes after stimulation. The pattern of arachidonic acid metabolites released is different from that obtained when the cells are stimulated with a phagocytosable particle like zymosan or calcium ionophore A23187. The data show that the metabolism of arachidonic acid by cycloxigenase is diminished. In an unusual way the amounts of prostaglandin PGE2, 6-keto-prostaglandin PGF1 alpha and thromboxane TXB2 synthesized are similar.

Acetylation↗

PubMedDate not supplied

Effect of ethanol on arachidonic acid metabolism in macrophages.

The authors report preliminary data on the production of arachidonic acid metabolites by ethanol stimulated macrophages. They focus on the substantial implications for understanding the pathobiology of alcoholism.

Animals↗