Rabbits deficient in both c7 and c21 allotypes were found in a certain colony of partially inbred rabbits and appeared to lack IgG with gamma light chain. In this colony, the gamma-chain level in the serum was low as an inherited character, and the mode of hereditary transmission of group c allotype expression was not apparent from available breeding data. These rabbits and other random-bred rabbits under conditions of suppressed T-cell functions produced group c allotypes which otherwise never appeared.

Animals↗

PubMedDate not supplied

Evaluation of brompheniramine safety in pregnancy.

Explore the source record for details and available documents.

Abnormalities, Drug-Induced↗

PubMedDate not supplied

Persistence and differentiation of HTLV-I-transformed immature T-cells in HTLV-I carrier rabbits.

Two T-cell lines with immature phenotypes, CD4-8- and CD4+8+, were found among HTLV-I-transformed T-cell lines obtained during experiments using a rabbit model of adult T-cell leukemia. Persistence and differentiation of these clones in vivo were examined by retrospectively analysing a series of cell lines from individual animals and by adoptive transfer of these cells into syngeneic hosts. The double negative cell line did not differentiate and remained double negative in adoptive transfer into neonates and in long-term in vitro culture, while the double positive cell line differentiated both in vivo and in vitro into CD8+ cell, as if the fate of this cell line had been predetermined. Cells of the same clone as this double positive cell line persisted for more than one year in the peripheral blood of the animal. Long-term persistence was not observed for five cell lines of CD4 single positive phenotype, which were obtained from another carrier rabbit. These results suggested that HTLV-I-transformed immature T-cells persist in neonatally infected carriers, continuously giving rise to mature T-cells harboring HTLV-I.

Animals↗

PubMedDate not supplied

Emission of extremely strong magnetic fields from the head and whole body during oriental breathing exercises.

This article reports the result of an experiment that was designed to measure the biomagnetic field emanating from two individuals who were practising traditional Oriental Qi Gong breathing exercises. The biomagnetic field was measured with differential coils wound 80,000 turns, a magnetic needle compass and a digital electromagnetic wave detection device. It was found that an extremely strong magnetic field was emitted from the two individuals. One subject emitted a magnetic field at the level of 200-300 mT (2-3 mGauss) and the other at 0.13 mT (1.3 mGauss). In both cases, moreover, the magnetic needle compass rotated 30 degrees (this was tested 32 times). When the rotation of the needle occurred, a reproducible magnetic field of 800-1500 mT (8-15 mGauss) was indicated on the digital measuring device (this was tested 12 times). It is concluded that traditional Oriental Qi Gong breathing appears to stimulate an unusually large biomagnetic field emission.

Breathing Exercises↗

PubMedDate not supplied

Recovery from allotype suppression in rabbits and release from this suppression in specific immune response.

The recovery of allotypic expression in allotype suppressed rabbits was followed up in specific antibodies and in bulk immunoglobulins. The primary antigenic stimulation with BSA and/or MS2 phage was given at 4 weeks of age. In the early recovery group, where the recovery from suppression began at 11-13 weeks of age, specific antibodies bearing the suppressed allotype were found to be synthesized by the primary stimulation or with successive allotype was scarely expressed in specific antibodies despite its recovered expression in bulk immunoglobulins. The X-ray irradiation followed by the immunization procedures promoted the recovery of the suppressed allotype expression in anti-BSA antibody and in bulk immunoglobulins but not in anti-MS2 phage antibody. The results are discussed in terms of regulatory mecahnisms of immune response in allotype suppressed animals.

Animals↗

PubMedDate not supplied

Idiotype-related cellular events during the anamnestic immune response to Salmonella typhi in rabbits.

Himalayan rabbits of a closed colony were immunized with injections of heat-killed Salmonella typhi and antiidiotypic antibodies against the induced anti- S. typhi antibodies were produced in rabbits of the same colony as well as in random-bred rabbits. Rabbits of the closed colony showed no proliferative response in a mixed lymphocyte culture of peripheral blood. Antiidiotypic sera from Himalayan rabbits recognized the idiotype in the corresponding immunizing sera alone, while one of sera from random-bred rabbits showed a cross-reaction with 8 out of 10 anti-S. typhi sera of Himalayan rabbits but not with any of 10 anti-S. typhi sera of unrelated random-bred rabbits. The cross-reactivity of the antiserum remained intact after absorption with unrelated immune precipitates. With this antiidiotypic serum the immunizing serum formed a bimodial arc at beta-gamma mobility and all other anti-S. typhi sera containing the cross-reactive idiotype a single arc at beta mobility. Solubilized immune precipitates of cross-reactive idiotype-anti-idiotype reaction could bind radiolabelled lipopolysaccharide (LPS) from S. typhi, but this anti-LPS activity was not revealed in an isoelectric focusing analysis. Gel analysis showed that the cross-reactive idiotype was located mainly in the macroglobulin fractions. The idiotype in the serum of the immunizing rabbits diminished and then was undetectable following the 2nd and the 3rd immunizations. When 3H-TdR uptake was examined in a mixed cell culture of peripheral blood from immunizing and antiidiotypic rabbits, there was a fluctuation in the proliferative response with two peaks occurring at a 4-week interval. An analysis of such a proliferative response was carried out by separating leukocytes and plasma from blood of the immunizing and the antiidiotypic rabbits. Mixed culture of cells alone did not produce a proliferative response, while culture of cells from the immunizing rabbit together with antiidiotypic plasma resulted in a potent reaction, irrespective of the presence of plasma from the immunizing rabbit. Presence of cells from the antiidiotypic rabbit in the culture inhibited this proliferative response. A fluctuation in the proliferative response to antiidiotypic serum was also observed with peripheral blood leukocytes (PBL) from rabbits producing the cross-reactive idiotype, while the antiidiotypic serum did not stimulate cells from rabbits which had been similarly immunized with S. typhi but did not produce the idiotype. PBL from the immunizing rabbit where the idiotype production ceased following the tertiary immunization were found to suppress definitely the proliferative response induced by the cross-reactive idiotype-anti-idiotype reaction. The suppressive activity was lost in PBL from the same rabbit after a cortisone treatment and the following antigenic stimulation of the animal led to reappearance in the serum of the idiotype. These results support the immune regulatory model which involves idiotype-anti-idiotype interactions.

Animals↗

PubMedDate not supplied

Cycling in the idiotypical proliferative response of peripheral blood lymphocytes in normal and Salmonella typhi-immunized rabbits.

A partially inbred rabbit was immunized with repeated intravenous administrations of Salmonella typhi during a two year period, and three antiidiotypic sera were prepared against anti-S. typhi antibodies. An idiotype which was detectable by a heterologous precipitation reaction with one of the antiidiotypic sera and which was associated with anti-S. typhi antibodies was found using a radiobinding inhibition test in the normal serum from all rabbits examined in the colony, but not in any of unrelated rabbits. Peripheral blood lymphocytes from the idiotypic proband rabbit showed a cycling in the proliferative response with all the three antiidiotypic sera. The response with two of the three antisera subsided following repeated immunizations, with concomitant decline in the idiotype level in the serum, whereas the response with other serum was observed even after the lapse of 6 months after the repeated immunizations, the idiotype persisting in the serum. Peripheral blood lymphocytes from normal rabbits of the colony also showed a fluctuation in the proliferative response with the antiserum against the cross-reactive idiotype and expressed at intervals a suppressive activity on the proliferative response when mix-cultured with the responding lymphocytes in the presence of the antiidiotypic serum.

Animals↗

PubMedDate not supplied

Further characterization of milk pyroglobulin.

Milk pyroglobulin was capable of passing through the "Visking" 8/32 cellulose membrane, when dialysed against deionized water but not against saline. Gel filtration analysis of the protein suggested its dissociation into subunit molecules in deionized water. Amino acid composition revealed high contents of proline, glutamic acid, valine and leucine. The protein possessed a biological activity to increase the permeability of skin vessels when injected intradermally. The colostral whey which was experimentally freed from heat-coagulable substances contained as major components proteins which are antigenically related to the pyroglobulin.

Amino Acids↗

PubMedDate not supplied

Influence of moxibustion on collagen-induced arthritis in mice.

The influence of moxibustion, a traditional Chinese medical treatment; on type II collagen-induced arthritis (CIA) was examined in DBA/1J mice in vivo. Mice were immunized intradermally twice at 3-week intervals with bovine type II collagen (C II). The main incidence of arthritis started around day 30 and lasted to day 60 after the first immunization. Moxibustion, using three different regimens, was applied at the acupoint equivalent to GV4 every other day. Moxibustion, from day 0 to day 30 after the first immunization, suppressed the onset and development of arthritis, as well as anti-collagen antibody level. Treatment with moxibustion, from the day 31 to day 60, also resulted in a significant inhibition of progression of arthritis and production of anti-C II antibody. Furtherfore we examined the influence of moxibustion on the established arthritis. Moxibustion given from day 61 to day 120, mildly but significantly decreased the anti-C II antibody level in diseased mice, while the bone erosion and joint destruction were not affected. These results indicate that moxibustion could prevent the incidence and attenuates the development of murine CIA.

Animals↗