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Biomedical subjects

A Shannon

Publications and source records attributed to A Shannon.

At least 19 recordsLinked to original sources
PubMed1998

The replicative intermediate molecule of bovine viral diarrhoea virus contains multiple nascent strands.

The replication of bovine viral diarrhoea virus (BVDV) RNA is considered to involve replicative intermediates (RI) and replicative forms (RF) of virus RNA. These RNA species were radiolabelled metabolically by 3H-uridine in BVDV-infected cells and separated by sucrose gradient centrifugation. RNA in the fractions was then digested with RNase A in high salt (2 x SSC) and the RNase-resistance was determined. Using the Baltimore formula, this led to the calculation that the RI of BVDV contained 6-7 nascent strands on the template. This suggests that the structure of the BVDV RI is similar to that of poliovirus and dengue 2 virus.

Animals↗
PubMed1998

A stable cell line with a proportion of cells persistently infected with bovine viral diarrhoea virus.

Bovine turbinate (BTu) and lamb testis (LT) cell lines persistently infected with bovine viral diarrhoea virus (BVDV) arose as a result of a single change of medium containing commercial foetal calf serum. Infected cells comprise 30% and 50% respectively, of the total cell population, determined by immunohistochemical staining. The ratio of positive cells has remained unchanged during successive passages. Characterization of the persistently-infected BTu cells (BTuI) showed that only full length viral RNA was detected by northern blot hybridisation, indicating that DI particles were not involved. Secreted and intracellular virus from these cells was fully infectious for fresh BTu and LT cells. The BTuI cell line was fully permissive for a cytopathic BVDV isolate and a bovine herpesvirus, but non-permissive for two non-cytopathic BVDV isolates. Attempts to induce the permissive state in the BVDV-negative cells of the BTuI culture by treatment with actinomycin D and 5'-aza-cytidine failed. These cells provide a convenient model to study aspects of BVDV pathogenesis and replication.

Animals↗
PubMed1998

Smile enhancement using indirect veneers.

The anterior 8 veneers provided the patient with a much improved appearance. She was more self confident and happy with her smile. It was great to see the immediate gratification cosmetic procedures can have on a people. It was also great to receive a sincere thanks for really making a difference.

Adult↗
PubMed1997

Clinical guidelines for indirect resin restorations.

Ongoing advances in adhesive dentistry have made it possible to successfully and predictably bond tooth-supporting restorations using conservative preparation techniques. Improvements in the durability and esthetic properties of tooth-colored restorative materials have also increased the range of available treatment options. However, dentists have been slow to accept both direct and indirect posterior esthetics. This article provides a step-by-step technique for practitioners who choose to treat their patients with indirect resin esthetic restorations. It will not discuss other posterior restorative treatment techniques or materials (i.e. gold, porcelain, amalgam, bonded amalgam, or direct resin).

Adhesives↗
PubMed1997

Indirect posterior restorations: steps to clinical success.

Continued advances in adhesive dentistry allow predictable bonding of tooth-supporting restorations using conservative preparation. As tooth-colored restorative materials continually yield improved durability and esthetic properties, treatment options expand. There has been slow acceptance of both direct and indirect posterior esthetics. The focus of this article is to review clinically proven techniques that when followed will consistently result in long-term functional indirect restorations.

Bicuspid↗
PubMed1994

INMAGIC update.

Explore the source record for details and available documents.

Journal Article↗
PubMed1991

Isoniazid resistant tuberculosis in a school outbreak: the protective effect of BCG.

An outbreak of isoniazid resistant tuberculosis occurred in a large second level school. A total of 1,160 teenage pupils were at risk. Nineteen cases of tuberculosis were diagnosed, 15 were students, 9 of whom were among 251 non-vaccinated students and 6 among 909 vaccinated students. Two cases of miliary tuberculosis, one of whom also had tuberculous (TB) meningitis, occurred in the non-vaccinated group. The number of children with Heaf grade +3 or +4 was significantly greater among children who had been given Bacille Calmette-Guérin (BCG) vaccination (8 vs 4.4%). This suggests a boosting effect on the response in vaccinated children. The protective effect of neonatal BCG vaccination in this school outbreak suggests that it provides significant protection against tuberculosis lasting into adolescence.

Adolescent↗