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A Shrift

Publications and source records attributed to A Shrift.

26 records · Page 2Linked to original sources

Selenium absorption by excised Astragalus roots.

Absorption of selenate and selenite by excised roots of Astragalus Crotalariae, a selenium accumulator, and of A. lentiginosus, a non-accumulator, was favored by CaCl(2) and a pH of 4.0. The uptake of selenate and possibly selenite, is metabolically linked. Roots of a number of Astragalus species were examined, and in all cases selenate entered the roots much faster than selenite. In these short-term experiments there was no relation between uptake of the 2 ions and classification of a species as selenium-accumulator or non-accumulator.

Absorption↗

Methionine transport in Chlorella vulgaris.

Absorption of (35)S-l-methionine by Chlorella vulgaris was measured at concentrations that ranged from 0.1 to 10.0 mumoles/ml. A brief, rapid phase of uptake was followed by a more prolonged, slower phase that was linear only at the lowest concentrations. The radioactivity accumulated by the end of 1 hour's incubation at an exogenous level of 0.1 mumole/ml was retained by the cells despite the inclusion of 10 mumoles/ml of nonradioactive methionine in the rinse medium. As the exogenous concentration was raised, the ratio of intracellular soluble radioactivity to exogenous radioactivity decreased. Analysis of the accumulated, soluble radioactivity showed that 90% was in the form of methionine and that about 10% had been converted to a compound with properties of S-adenosylmethionine. Azide and ethionine were the most effective of the inhibitors tested.

Biological Transport, Active↗