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A Signorella

Publications and source records attributed to A Signorella.

5 recordsLinked to original sources

Feedback effects of mammary gland tumors on the host pituitary prolactin cell.

Prolactin (PRL) cells in pituitary glands of F-344 female rats bearing transplantable mammary gland adenocarcinomas (13762, 3230) were smaller, contained less intracellular hormone, and released less hormone in culture relative to non tumor-bearing littermates. In some instances removal of the tumor by surgery or chemotherapy (PAM) resulted in restoration of function in vitro. On the other hand, PRL cells from pituitaries of breast cancer patients were often hypertrophied. Since these patients had mastectomies prior to hypophysectomy, the data are consistent with the hypothesis that the tumor exerts negative feedback at the pituitary or hypothalamic level. In support of this hypothesis we have discovered a potent PIF in rat serum. In addition, an experimental protocol involving a) encapsulation of pituitary cells in Amicon hollow fibers, b) their implantation in ectopic sites and c) their subsequent in vitro culture yields data which suggest that the brain of the tumor bearing rat suppresses PRL release. It is suggested that decreased PRL cell function in animals bearing certain mammary tumors ultimately favors metastatic activity of the tumor cell.

Animals↗

Rat serum inhibits prolactin release from rat pituitary mammotrophs in culture.

Rat pituitary cells maintained in culture for 3 days in alpha-MEM supplemented with 17% horse serum produce and release 4-8X the quantity of prolactin (PRL) originally contained in the cells at the time of plating. Addition of small (200 microliter) quantities of rat serum repeatedly suppressed PRL release by approximately 50%. Inhibition was related to dose of rat serum. Other studies showed (a) that the cells could recover from serum-induced inhibition, (b) that the effect was independent of the age of the pituitary and/or serum donor, (c) that GH release was unaffected by rat serum addition, (d) that the effect could not be attributed to proteases in the serum preparations and (e) that addition of T3 to the culture medium prevented the inhibitory response. The inhibitory material(s) in rat serum is stable on freezing; is non-dialyzable; is heat-labile; is stable over 3 days incubation at 37 degrees C; and is associated with molecules between 85 000 and 146 000 dalton. Inhibitory activity was generated upon incubating whole clotted blood at 37 degrees C for 23 h. It is suggested that the inhibitory material might (a) be released from platelets during clotting or (b) be generated from an inactive precursor molecule cleaved by proteases released during the clotting process.

Aging↗

Coil springs.

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Dentistry↗