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A Slomiany

Publications and source records attributed to A Slomiany.

At least 343 records · Page 19Linked to original sources
PubMed1978

Isolation and characterization of the sulfated neolactotetraosylceramide from hog gastric mucosa.

A sulfated neolactotetraosylceramide has been isolated from the lipid extract of hog gastric mucosa. Isolation procedure involved extraction of lipids with 0.4 M sodium acetate in methanol/chloroform/water, alkaline methanolysis, column fractionation on DEAE-Sephadex, separation of the acetylated acidic glycolipids on silicic acid column, and thin layer chromatography. By partial acid hydrolysis, sequential hydrolysis with specific glycosidases, periodate oxidation, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: Galbeta1 leads to 4GlcNAc(6 comes from SO3)beta1 leads to 3Galbeta1 leads to 4Glc1 leads to 1 ceramide.

Animals↗
PubMed1978

The nature of ABH blood group antigens in human gastric secretion.

The origin of blood group ABH activity in human gastric content was investigated. Dialyzed and lyophilized samples of ten individual gastric secretions were assayed for ABH antigen under various conditions. The native activity persisted in delipidated residue of the respective secretions, but was completely missing in the lipid extracts of the analyzed samples. The alkaline degradation of the native and delipidated samples led to total loss of blood group activity of the analyzed materials, but no effect on A-active glycosphingolipid was evolved. Purified glycolipid portion of the lipid extract was lacking ABH activity and was shown to have distinct composition. This fraction contained only glyceroglucolipids and neither sphingosine nor other carbohydrates were present. On the basis of blood group activity assays of the native, delipidated, alkaline degraded samples and also on glycolipid analysis it was established that the ABH blood group activity of stomach secretion originated entirely from the glycoprotein portion of these samples.

ABO Blood-Group System↗
PubMed1978

Forssman glycolipid variants of dog gastric mucosa. Structure of a branched ceramide octasaccharide.

A fucose-containing ceramide octasaccharide exhibiting Forssman antigenic activity, and reacting in human H anti-H and anti-A systems, was isolated from water-soluble glycolipids of dog gastric mucosa. Defucosylation of the glycolipid resulted in the loss of H-activity, but had no effect on its Forssman nor blood-group A antigenic activity. The branched structure of glycolipid was identified by partial acid hydrolysis, sequential degradation with specific glycosidases and comparison of the permethylation products of the native and enzyme-degraded compound. The structure of this glycolipid is proposed to be: formula.

Animals↗
PubMed1978

Glyceroglucolipids of the human saliva.

Seven individual glycolipids (I--VII) have been isolated from the lipid extract of human saliva. All glycolipids contained glucose, glyceryl ethers and fatty acids, and differed from each other primarily with respect to the number of glucose residues. In addition, glycolipid V contained also the sulfate ester group. The structures of these glycolipids were identified by partial acid and alkaline hydrolysis, oxidation with periodate and chromium trioxide and methylation studies, as: Glc(alpha1 leads to 3)-diglyceride (glycolipid I), Glc(alpha1 leads to 6)Glc(alpha1 leads to 3)-diglyceride (glycolipids II and III), Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 3)-diglyceride (glycolipid IV), SO3H-6Glc(alpha1 leads to 6)Glc(alpha1 leads to 3)-diglyceride (glycolipid V), Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 3)-diglyceride (glycolipid VI) and Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 6)Glc(alpha1 lead to 6)Glc(alpha1 leads to 6)Glc(alpha1 leads to 3)-diglyceride (glycolipid VII). Diglyceride portion of these compounds consists of 1-O-alkyl-2-O-acyl-glycerol with the docosanoate and glyceryl-monodocosyl being the predominant acyl and alkyl components.

Diglycerides↗
PubMed1978

ABH-blood-group antigens and glycolipids of human saliva.

The nature of ABH-blood-group antigens in saliva was investigated. Human saliva was examined serologically for ABH-blood-group activity in its native form and after various treatments. The activity of the native form persisted in the delipidated samples, but was entirely lost after alkaline degradation. The lipid portion of saliva was completely inactive in the ABH hemagglutination inhibition system. The same results were obtained when purified glycolipid fraction of saliva was used instead of whole lipid extract. Neither alkaline treatment nor excessive amounts of salivary lipids effected antigenic activity of A-active glycosphingolipids of hog gastric mucosa admixed to saliva samples before alkaline degradation and/or in presence of large amounts of salivary lipids. The isolated glycolipid fractions contained at least eight glycolipids, each of which was composed of glucose, glyceryl ethers and fatty acids and differed from others with respect to number of glucose residues. Sphingosine and sugar residues involved in formation of ABH antigenic determinants were not detected. These findings together with data on stomach secretion [1,2] led us to the conclusion that ABH-blood-group antigens of saliva are exclusively of glycoprotein nature.

ABO Blood-Group System↗
PubMed1978

Studies on the occurrence of disialosyl groups in glycoproteins of salivary glands.

The major glycoprotein of pig submandibular and rat sublingual glands were investigated for the presence of disialosyl groups. The content of (C-8)-substituted sialic acid in the glycoproteins was determined by three different procedures: colorimetric, periodate oxidation and permethylation. Approximately 13% of sialic acid in pig submandibular glycoprotein, but none in rat sublingual glycoprotein, contained a substituent at C-8. This substituent was identified as sialic acid from its susceptibility to neuraminidase treatment. From our estimation, it appears that one out of four sialic acid residues in pig submandibular glycoprotein occurs in an N-acylneuraminyl-alpha(2 leads to 8)-N-acylneuraminyl group.

Animals↗
PubMed1977

Characterization of two major neutral glyceroglucolipids of the human gastric content.

Two major neutral glyceroglucolipids (A and B) have been isolated from lipid extract of human gastric content by the procedure involving column fractionation on DEAE-Sephadex, silicic acid, and thin-layer chromatography. Both glycolipids contained glucose, glyceryl ethers, and fatty acids. The structures of these glycolipids were identified by mild alkaline methanolysis, oxidation with periodate and chromium trioxide, and permethylation studies. Based on the obtained data, we propose that glycolipid A is a monoalkylmonoacylglyceryl hexaglucoside and glycolipid B is a monoalkylmonoacylglyceryl octaglucoside. The diglyceride portion of these glycolipids consists mostly of 1-O-alkyl-2-O-acylglycerol.

Diglycerides↗
PubMed1977

Glycolipids of the human gastric content. Structure of the sulfated glyceroglucolipid.

A new sulfated glycolipid has been isolated from the lipid extract of human gastric content by the procedure involving column fractionation on silicic acid and thin-layer chromatography. Degradation studies revealed the presence of glucose, sulfate, fatty acids and glyceryl monoethers. The structure of this glycolipid was identified by partial acid hydrolysis, oxidation with periodate and chromium trioxide, and permethylation studies, as: SO3H-6Glcalphal leads to 6Glcalphal leads to 6Glcalphal leads to 3-diglyceride. The diglyceride portion of this glycolipid consists mostly of 1-O-alkyl-2-O-acyl-glycerol.

Chemical Phenomena↗
PubMed1977

Water-soluble glycosphingolipids of dog gastric mucosa. Characterization of a branched ceramide heptasaccharide.

Two glycosphingolipids, the carbohydrate portion of which consisted of galactose, glucose and N-acetylgalactosamine, have been isolated from the aqueous phase of buffered tetrahydrofuran extract of dog gastric mucosa. The structures of these glycolipids were identified by partial acid hydrolysis, sequential degradation with specific glycosidases and methylation analysis. The structure of glycolipid I (GalNAcalpha1 leads to 3GalNAcbeta1 leads to 3Galalpha1 leads to 4Galbeta1 leads to 4Glc leads to ceramide) was found to be identical to that of Forssman hapten. The branched structure of glycolipid II, as determined by the combination of enzymatic degradation and comparison of the permethylation products of the enzyme-degraded compounds, is proposed to be: See Source.

Acetylgalactosamine↗
PubMed1977

Branched blood group A-active fucolipids of hog gastric mucosa.

New complex glycolipids have been extracted from hog gastric mucosa with the mixture of 0.4 M sodium acetate in methanol/chloroform/water. Three A-active fucolipids having branched carbohydrate chains have been purified from this extract. The postulated structures of these glycolipids are based on the results of partial acid hydrolysis, oxidation with periodate and chromium trioxide, and permethylation studies.

ABO Blood-Group System↗
PubMed1977

Structural studies on branched fucosphingolipids of hog gastric mucosa.

A structural studies have been performed on new complex glycolipids extracted from hog gastric mucosa by 0.4 M sodium acetate in methanol-chloroform-water, and which were purified to homogeneity by DEAE-Sephadex and Florisil column chromatography and by preparative thin-layer chromatography in three solvent systems. Five branched fucolipids have been purified from this extract, three of which have been characterized previously [1] and remaining two were subject of this investigations. Based on the results of partial acid hydrolyses, oxidation with periodate and chromium trioxide, permethylation and serological activities following structures were proposed.

Animals↗
PubMed1976

The glycosphingolipids of rat sublingual and submaxillary glands.

1. Glycosphingolipids have been isolated from rat sublingual and submaxillary glands by the procedure involving lipid extraction, column fractionation and thin-layer chromatography. 2. The major neutral glycosphingolipids in rat sublingual and submaxillary glands were monohexosylceramide, dihexosylceramide, tetrahexosylceramide and pentahexosylceramide. Both types of glands exhibited a low content of trihexosylceramide. The fucose-containing glycosphingolipids were not found. 3. The acidic glycosphingolipids in rat sublingual and submaxillary glands were composed of monohexose sulfatide, dihexose sulfatide and monosialo-and disialogangliosides of hematoside series. In addition, small quantities of gangliosides containing hexosamines were also present. 4. The distribution of acidic and neutral glycosphingolipids was similar in the sublingual and submaxillary glands, except for the tetrahexosylceramide and sultatides. Sublingual glands contained 1.5 and 3.0 times as much tetrahexosylceramide and sulfatides, respectively, as did submaxillary glands. 5. The glycosphingolipids of submaxillary and sublingual glands showed large similarity in fatty acid composition. The fatty acid composition of gangliosides resembled each other, but differed remarkably from those of sulfatides and neutral glycosphingolipids in the docosanoate content.

Animals↗