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Biomedical subjects

A Smit

Publications and source records attributed to A Smit.

28 records · Page 2Linked to original sources
PubMed1992

Plaque quantitation through protein measurement.

This study was undertaken to establish whether the quantitation of dental plaque protein by a dye-binding method (Coomassie G-250) may be used as an index of the amount of dental plaque sampled. Ten sites were sampled in 34 children on 5 occasions at 4 month intervals. The mean protein concentration in 1391 plaque samples was 6.9 +/- 4.1 micrograms (micrograms) (mean +/- standard deviation). A three-way analysis of variance showed that the plaque protein concentration was similar at the different sampling sites in the same child (p = 0.14), but statistically significant differences were observed with respect to time of sampling (F = 36.24; p = 0.0001) and individual sampled (F = 5.69; p = 0.0001). These observations indicate that plaque bacterial counts may be expressed as units of protein concentration and this method may be useful to relate the number of viable bacteria to an estimate of the amount of plaque collected. This ratio allows standardisation for any variation in the amount of plaque collected.

Analysis of Variance↗
PubMed1989

Structure and function of the androgen receptor.

The androgen receptor in several species (human, rat, calf) is a monomeric protein with a molecular mass of 100-110 kDa. The steroid binding domain is confined to a region of 30 kDa, while the DNA-binding domain has the size of approx. 10 kDa. A 40 kDa fragment containing both the DNA and steroid binding domain displayed a higher DNA binding activity than did the intact 100 kDa molecule. cDNA encoding the major part of the human androgen receptor was isolated. The cDNA contains an open reading frame of 2,277 bp but still lacks part of the 5'-coding sequence. Homology with the progesterone and glucocorticoid receptor was about 80% in the DNA binding domain and 50% in the steroid binding domain. The present data provide evidence that the androgen receptor belongs to the superfamily of ligand responsive transcriptional regulators and consists of three distinct domains each with a specialized function.

Amino Acid Sequence↗
PubMed1986

Plasma volume in black women with eclampsia.

Plasma volume was estimated in 11 black women with eclampsia and compared with 9 normotensive primigravidas. With respect to age, weight and height the two groups were similar and although plasma volume was 4.1% less in the reference group, this difference was not statistically significant.

Eclampsia↗
PubMed1982

Practical considerations in the positioning of EEG electrodes.

Although the 10-20 system of the International Federation was originally proposed as a basis for discussion, there has been little published comment on its strengths and weaknesses or suggestions for its improvement. There are inherent ambiguities in the system, which cannot be applied strictly in accordance with the published specification unless the 4 measurements between the right and left preauricular points and the nasion and the inion are all equal. A study of the cranial measurements of 20 normal adults showed that this criterion was fulfilled to within a margin of 10% in less than half. Further, the standard placements of the 10-20 system cover less than 3/4 of the cerebral convexity. A study of fronto-temporal epileptiform phenomena in 100 subjects indicated that in the majority these were of maximum amplitude over the anterior temporal region, some 20 mm below the typical positions of F7 and F8. In the light of these and previous studies, modifications of the 10-20 system are suggested.

Adult↗
PubMedDate not supplied

Training primary care physicians improves the management of depression.

The purpose of this pretest-posttest study was to evaluate effects of a training program designed to improve primary care physicians' (PCPs) ability to recognize mental health problems (MHP) and to diagnose and manage depression according to clinical guidelines. The primary care settings were in the northern part of The Netherlands. There were eight intensive, hands-on training sessions of 2.5 hours, each of which three were targeting depression (7.5 hours). In the pretraining phase we screened 1778 consecutive patients of 17 PCPs with the 12-item General Health Questionnaire (GHQ-12) and interviewed a stratified sample of 518 patients about presence of current depression with the Primary Health Care version of the Composite International Diagnostic Interview (CIDI-PHC). PCPs registered patient's mental health (status, severity, diagnosis) and treatment prescribed. Then we trained the PCPs. In the posttraining phase, we screened a new group of 1724 consecutive patients of the same PCPs and a new stratified sample of 498 patients went through the same interview and rating procedures as patients in the pretraining phase. Knowledge about depression was assessed pre- and posttraining. PCPs' knowledge of depression improved significantly. Recognition of MHP and accuracy of depression diagnosis improved, but was not statistically significant. The proportion of patients receiving treatment according to the clinical guidelines increased significantly. It was observed that training PCPs improves the management of depression.

Cohort Studies↗