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Biomedical subjects

A Sobek

Publications and source records attributed to A Sobek.

35 records · Page 2Linked to original sources
PubMed1993

Low dose spironolactone in the treatment of female hyperandrogenemia and hirsutism.

Thirty one women with hyperandrogenism, clinically divided into polycystic ovary syndrome (PCOS)--28 women and idiopathic hirsutism (I.H.)--3 women, were treated with low dose spironolactone (50 mg or 75 mg daily) for average five months. There was an excellent clinical response in 19 (61%), incomplete response in 8 (26%), no response in 5 women. Six of 18 patients with sterility became pregnant during the one year after treatment and delivered a healthy infant at term. Two patients dropped out of the trial because of intolerance of the therapy. Remarkable change of the menstrual pattern characterised as polymenorrhea was major side effect of the therapy. Other side effects were not problem. Spironolactone caused statistically significant reduction in testosterone, luteinizing hormone and prolactin values at the end of the treatment. Our results demonstrate that low-dose spironolactone is effective in the treatment of hyperandrogenism in women.

Adolescent↗
PubMed1991

Monitoring of ovulation by ultrasound.

Ovarian growth was monitored by ultrasound and plasma hormonal levels (E2, LH, FSH, progesterone) in 124 cycles of 71 patients. Providing a direct view of follicular maturation, ultrasound can help in the determination of the best time for the induction of ovulation. Examining the speed of follicular growth we can predict some ovulatory dysfunction.

Female↗
PubMed1991

Metoclopramide test in women with PCO syndrome after ovarian wedge resection.

Afternoon serum PRL levels and PRL responsiveness to metoclopramide (MCP) were determined in 36 women, aged 30.5 +/- 4.5, with normoprolactinemic anovulation. All women underwent a bilateral ovarian wedge resection with diagnosis polycystic ovarian disease (PCO) 2.9 +/- 2.0 years ago. After operation only four women had been pregnant. A bolus i.v. dose of 10 mg metoclopramide was given and serum PRL was estimated before, 30 and 60 min. after MCP administration. Diurnal serum PRL levels were approximately 9 ng in all patients. The PCO patients were classified into 2 groups in terms of the responsiveness to metoclopramide test. MCP induces rapid and marked elevation in serum PRL levels in all subjects. The maximum post MCP PRL value in the group I patients (n = 16) was 143.0 +/- 37.7 ng/ml, which was significantly higher than the maximum value in the II group patients (104.3 +/- 32.5 ng/ml) (P less than 0.005). Nine (56.2%) of the I group patients had maximum PRL values higher than 150 ng/ml; the proportion was statistically higher than 10 percent maximum PRL values in the group II (P less than 0.01). This finding suggests that the patients who had enhanced PRL responsiveness to MCP test have latent hyperprolactinemia, which can not be detected by analyzing PRL levels in blood samples taken randomly. This latent hyperprolactinemia presumably might be normalized by dopamine agonist therapy, resulting in resumption of ovulatory cycles in these women.

Adult↗
PubMedDate not supplied

Phosphoamino acids in proteasome subunits.

Proteasomes, the major catalysts of the non-lysosomal proteolytic pathway in eukaryotic cells, were analyzed for their content of phosphoamino acids using polyacrylamide gel electrophoresis and subsequent detection on Western blots by phosphoamino acid antibodies. No specific binding to proteasome subunits was observed with phosphoserine or phosphothreonine antibodies, whereas phosphotyrosine antibodies were bound by a single proteasome subunit, which was identified in rat as well as in human proteasomes as subunit C7-1. Since dephosphorylation of the subunit by phosphatases was not possible, analysis of phosphoamino acid content of all proteasome subunits was performed using another method. All proteasome subunits were isolated from 2D-polyacrylamide gels and subjected to partial acid hydrolysis. Phosphoamino acids were subsequently detected by capillary electrophoresis after their derivatization with phenylisothiocyanate. This analysis revealed no phosphorylated amino acid in subunit C7-1, however, subunit C3 contained phosphotyrosine and phosphothreonine, and phosphoserine was detected in subunits zeta, C5, C8 and C9.

Amino Acids↗