The authors examined 263 children and adolescents aged 5-20 years who suffered from so-called non-ulcerative dyspepsia or epigastric pain, because of the suspected presence of Campylobacter pylori. In 31.9% of the examined subjects direct microbiological methods revealed its presence in the gastroduodenal mucosa. The confidence limit of positive results of Campylobacter pylori in the population is between 27.58% and 38.84%, the probability being 95%. The authors proved a mutual correlation between endoscopic, histological, serological findings and detection of Campylobacter pylori by microbiological methods. The statistical significance of the correlation of campylobacter pylori and chronic gastritis B (mostly inactive) provides further support for the hypothesis of the aetiological role of Campylobacter pylori in the development of chronic gastritis B in children and adolescents. From the investigation it does not ensue, however, that colonization with Campylobacter pylori is associated with certain clinical symptoms in all instances. However, in the differential diagnosis of so-called non-ulcerative dyspepsia and epigastric pain in children and adolescents we must include infection with Campylobacter pylori among their possible causes.

Adolescent↗

PubMedDate not supplied

[Cytotoxic changes in cell cultures induced by newly isolated strains of Campylobacter pylori].

Suspensions of primocultures of two strains of Camplyobacter pylori, isolated from bioptic materials collected from patients with nodular hyperplasia of the pyloric antrum and with gastric ulcers were inoculated to 20-hour confluent or almost confluent cell cultures line VERO, McCoy and MA 104 in test tubes and Petri dishes. Both strains of C. pylori adhered markedly to cell lines VERO and McCoy and after 72 hours led to marked morphological changes of the cells. The changes were characterized in VERO cells by the presence of polygonal to star-shaped cells with processes, a reduced cytoplasm and only in the final stages by the formation of circular cells separating from the medium. Some cells were vacuolized. Cells of the McCoy line responded to the infection with C. pylori by a similar morphological picture, changes in the cell periphery were, as compared with the VERO line, more marked in the early stages. Cells of line MA 104 did not respond to infection with C. pylori by any morphological changes, not even after six days. The intensity of morphological changes was more marked in cell cultures line VERO infected with a strain isolated from a patient with a gastric ulcer, as compared with changes caused by a strain of C. pylori isolated from a patient with nodular hyperplasia of the pyloric antrum. The possibility to induce morphological changes of some stable lines supports the idea of the pathogenic action of Campylobacter pylori on the gastric mucosa.

Cell Line↗

PubMedDate not supplied

[Determination of antibodies to Campylobacter pylori in children and adolescents with gastroduodenal disorders].

Serological examination of antibodies against Campylobacter pylori was made in 195 boys and girls aged 9-20 years. In all on account of gastrointestinal complaints endoscopy was indicated. The results of microbiological and histological finding), in particular in the antrum and corpus of the stomach). Positive titres antrum and body of the stomach were compared with results of the complement fixation test and the ELISA reaction. Antibodies against C. pylori were detected in 64 of the examined subjects. The titres assessed by the complement fixation and ELISA test were in good agreement. Patients with positive sera had significantly more often a positive endoscopic finding (bacteria, chronic gastritis) and bacteriological finding), in particular in the antrum and corpus of the stomach). Positive titres of the complement fixation reaction persisted frequently for 15 months after the first examination. From the results ensues that serological examination of antibodies against C. pylori can be considered a sparing examination method which in patients with gastrointestinal complaints should precede endoscopic examination and moreover it can be used to follow up the course of the disease and success of therapy.

Adolescent↗

PubMedDate not supplied

[Effect of non-specific immunostimulation on the functional state of RES in rats with a transplanted Yoshida sarcoma (author's transl)].

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Animals↗