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Biomedical subjects

A Stajner

Publications and source records attributed to A Stajner.

At least 19 recordsLinked to original sources
PubMed1996

[Use of endogenous proteins in the early diagnosis of dysfunction in transplanted kidneys].

The early diagnosis of renal allograft dysfunction in the immediate posttransplantation period is very important. The prospective study was undertaken in 60 patients after kidney transplantation. The significance of blood and urine levels of glomerular and tubular proteins was studied for early diagnosis of allograft dysfunction. The authors evaluate some glomerular (CRP, transferrin, immunoglobulins) and tubular (alfa 1 microglobulin and beta 2 microglobulin) proteins for differential diagnosis of acute tubular necrosis and acute rejection of transplanted kidney.

Adult↗
PubMed1989

[Immunologic risks in patients after splenectomy].

The more frequent incidence of infections after splenectomy, some leading even to OPSI syndrome, is known from the literature and practice. The authors investigated in two groups of 20 splenectomized patients (on account of injury of the spleen or for haematological reasons) in particular changes of immunological indicators. On both groups they revealed a predominating increase of the absolute number of leucocytes, lymphocytes, the phagocytic index of PMN leucocytes, the ratio of active nucleoli, FW. They found also in both groups a predominating reduction of the number of T lymphocytes (p less than 0.05), with a high probability also NK cells, and a reduction of the late type reactivity of the skin test (Immunoskin test USOL, Praha). In patients splenectomized on account of injury the number of B lymphocytes was raised in 50% of the patients. In both groups of patients IgG serum levels were elevated, alpha-1-antitrypsin and haptaglobin levels were reduced. The CRP level in patients with haematological diagnoses was elevated as compared with the group of patients splenectomized on account of injury (p less than 0.05). In both groups after splenectomy a greater susceptibility to respiratory infections was found. On examination patients with a greater risk were detected and included under dispensary care.

Adult↗
PubMed1982

Migration of leukocytes under agarose in the presence of some polymers.

When investigating migration and chemotaxis of leukocytes under agarose polyvinylpyrrolidone (PVP; 11 kg/mol) and polyethyleneglycol (PEG; 6 kg/mol) were used instead of serum. The highest migration was detected with 0.5-1 mm of PVP. Phagocytosis was not influenced either by PVP or PEG within a concentration range of 0.01-1 mm.

Cell Movement↗
PubMed1980

Modification of fat and carbohydrate metabolism by neurohypophyseal hormones. I. Effect of lysine-vasopressin on non-esterified fatty acid, glucose, triglyceride and cholesterol levels in the serum of female rats.

The effect of the subcutaneous administration of lysine-vasopressin, in doses of 0.625, 1.25, 2.5 and 5 I.U./kg on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting female rats was determined. All the doses, in both fed and fasting animals, caused a marked drop in non-esterified fatty acid levels about 15 min after starting the experiment. The only exceptions were fed females given doses 2.5 and 5 I.U./kg, in which non-esterified fatty acid levels rose. Apart from animals given the smallest dose (0.625 I.U./kg), glucose levels rose during the same interval. In the case of triglyceride levels, a dose of 0.625 I.U./kg produced a decrease and the larger doses (1.25, 2.5 and 5 I.U./kg) an increase in fed females. In fasting animals, triglyceride levels fell (statistically non-significantly) after doses of 0.625, 1.25 and 5 I.U./kg, and rose after 2.5 I.U./kg. The cholesterol level showed a temporary tendency to fall in most of the experiments.

Animals↗
PubMed1980

Modification of fat and carbohydrate metabolism by neurohypophyseal hormones. II. Effect of lysine-vasopressin on non-esterified fatty acid, glucose, triglyceride and cholesterol levels in the serum of male rats.

The effect of subcutaneously injected lysine-vasopressin on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting male rats given doses of 2.5 and 5 I.U./kg was determined. About 15 min after starting the experiment, non-esterified fatty acid levels abruptly fell and glucose levels rose in both fed and fasting animals. The only exception were fed males given a dose of 5 I.U./kg in which there was a delayed increase in the non-esterified fatty acid level. Triglyceride levels rose in fed males and fell in fasting males. The cholesterol level fell in both fed and fasting animals.

Animals↗
PubMed1980

Modification of fat and carbohydrate metabolism by neurohypophyseal hormones. III. Effect of oxytocin on non-esterified fatty acid, glucose, triglyceride and cholesterol levels in rat serum.

The authors determined the effect of s.c. injected oxytocin on serum non-esterified fatty acid, triglyceride, cholesterol and glucose levels in fed and fasting rats of both sexes, in doses of 2.5 and 5 I.U./kg. No changes in non-esterified fatty acid levels were found in fasting animals of either sex. In fed males, non-esterified fatty acid levels rose abruptly after both doses; in fed females they fell after the larger dose. In fed animals of both sexes, the smaller dose of oxytocin produced a decrease, and the larger dose an increase, in triglyceride levels. In fasting females, triglyceride levels fell after both doses; in fasting males a temporary increase was found after the larger dose. In the great majority of cases cholesterol levels displayed a tendency to fall, in both males and females. In the case of glucose, a transient increase was recorded in fed animals of both sexes after the smaller dose and a decrease in males after the larger dose. In fasting animals, glucose levels temporarily rose in every case.

Animals↗
PubMed1977

The determination of nonesterified fatty acids in blood serum using a stable cupric reagent.

A modification of the method for the determination of nonesterified fatty acids in blood serum using a stable cupric reagent stabilized with sodium citrate, has been developed. 33 g of NaCl are dissolved in 100 ml of the described reagent, and its pH should not be additionally adjusted. The reagent is stable for at least one year if kept in the refrigerator at 5 degrees C. The determination may be done in 0.1--0.2 ml of blood serum. The relationship between the absorbance and concentration of palmitic acid is still linear at a concentration of 2500 mumol/l. Error of the method is +/- 3%.

Citrates↗