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Biomedical subjects

A Staubli

Publications and source records attributed to A Staubli.

10 recordsLinked to original sources
PubMed1989

Direct visualization of an IgM directed against a membrane antigen involved in both cell-matrix interactions and microfilament organization.

Dental mesenchymal cells were cultured in the presence of a monoclonal antibody. MC16A16, consisting of IgM and directed against a hydrophobic 165 kDa protein. Since the epitope recognized by MC16A16 was found to be at the outer cell surface, a direct visualization of IgM antibodies was used to localize the 165 kDa antigen by transmission electron microscopy. The present results demonstrate that the 165 kDa antigen is a membrane protein.

Actin Cytoskeleton↗
PubMed1989

Immunoperoxidase localization of fibronectin during odontoblast differentiation. An ultrastructural study.

Immunoperoxidase labeling of fibronectin in one-day-old mouse first lower molars allowed to visualize a striking redistribution of this glycoprotein during terminal cytodifferentiation of odontoblasts. The modifications involved both extracellular and cell surface localizations. The possible roles of these modifications in terminal differentiation of odontoblasts are discussed.

Animals↗
PubMed1988

Lipid detection by malachite green-aldehyde in the dental basement membrane in the rat incisor.

Developing rat incisors were treated with malachite green-aldehyde fixative solution (MGA), which retains and stains lipids. We observed positive staining occurring as dots in the basement membrane. Most of these dots (2-3.5 nm in diameter) were grouped in the lamina densa but some were also present in the lamina lucida and the lamina fibroreticularis. These data provide evidence for the existence of lipids in the dental basement membrane and suggest that they are distributed together with the various groups of proteins so far detected.

Aldehydes↗
PubMed1988

Effects of glycosaminoglycans on in vitro mouse dental cells.

Trypsin-dissociated dental papillae and enamel organs removed from the first lower molars of day-18 and day-19 mouse embryos were cultivated for 2 to 4 days on Millipore filters in DMEM supplemented with 15 per cent fetal calf serum and chondroitin sulphate and hyaluronic acid, individually or together. Three concentrations of glycosaminoglycans (GAGs) were also added to the media (01, 0.2, 0.4 mg/ml). Control cultures were made in the absence of GAGs, and additional experiments performed in which the presence of GAGs was associated with serum-free medium. Elongated and polarized odontoblasts showing synthetic activity were only observed in the presence of serum containing medium supplemented with 0.1 or 0.2 mg/ml of the GAGs. [3H]-thymidine autoradiography demonstrated that these cells were already post-mitotic at the onset of the culture. Polarized ameloblasts were never observed. These data provide evidence that GAGs are able to maintain the polarized state of cultured odontoblasts.

Ameloblasts↗
PubMed1988

Ultrastructural duality of extracellular fibrillar components of the odontoblast layer in the mouse molar.

The application of detergent solutions to dissociated molar dental papillae of mice permitted precise observation of the extracellular fibrillar material in the odontoblast layer and the predentine. Two types of fibrils were identified: cross-banded fibrils of variable diameter (25-200 nm) and aperiodic microfibrils of regular diameter (12.5 nm) and undetermined length. If the collagenic nature of the cross-banded fibrils is evident, the composition of the aperiodic microfibrils is probably different.

Animals↗
PubMed1979

[Anterior interbody fusion with neurological symptoms of a spondylolisthesis (author's transl)].

28 patients with spondylolisthesis who underwent anterior interbody fusion at the Orthopaedic Department of the University of Basle between 1968 and 1976, have been follow-up. In 90% satisfactory or good clinical results were achieved. In 82% the neurological symptoms - without laminectomy - were markedly improved or eliminated. According to the authors anterior interbody fusion (with reduction and enlargement of the intervetebral space) is indicated even when neurological symptoms are present.

Follow-Up Studies↗
PubMed1978

Basement membrane reconstitution and cytodifferentiation of odontoblasts in isochronal and heterochronal reassociations of enamel organs and pulps.

The restoration of the basement membrane and the terminal differentiation of odontoblasts were studied in iso- and heterochronal reassociations between dental mesenchyme and enamel organs. It was suggested that the cytodifferentiation of odontoblasts was triggered by the basement membrane secreted by the enamel organ of a specific stage.

Animals↗
PubMed1977

[Cellular relations during odontogenesis].

Epithelial-mesenchyme interactions during mouse molar tooth morphogenesis were observed by electron microscopy. The basal lamina was intact from day 10 to day 18. Aperiodic, later also periodic fibrils were deposited on the basal lamina. Ectomesenchymal cell processes were always in close relation with the basal lamina or with the fibrillar material. On day 19 some direct heterotypic cell contacts between odontoblasts and preameloblasts were observed. At all stages, especially the preodontoblasts but also preameloblasts, gave rise to aggregated vesicles, which were extruded in the epithelial-mesenchymal space.

Ameloblasts↗
PubMedDate not supplied

Epithelial-mesenchymal interactions: effects of a dental biomatrix on odontoblasts.

The functional differentiation of odontoblasts requires specific interactions between these cells and the extracellular matrix. To further analyze these phenomena we studied the effects of a "dental papillae biomatrix" on isolated dental papillae cultured in vitro. The dental papillae biomatrix was extracted from EDTA-dissociated day-18 mouse dental papillae by homogenization, NaCl and enzymatic treatments, and deposited on Millipore filters. This biomatrix was studied by means of transmission electron microscopy and indirect immunofluorescence: it contained collagen fibrils, type IV collagen, fibronectin and laminin; cellular residues were also observed. The dental papillae were isolated by trypsin treatment of homologous tooth germs and cultured on uncoated (control) and coated filters. As shown by histological and cytological data, odontoblast-like cells never differentiated in control cultures. In presence of biomatrix and serum, polarized functional cells were observed. The functional state of these cells was enhanced by the addition of ascorbic acid to the culture media. Study of the incorporation of 3H-proline in cultured dental papillae and in macromolecules secreted into the culture media corroborated the morphological findings.

Animals↗