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Biomedical subjects

A Stuart

Publications and source records attributed to A Stuart.

At least 55 records · Page 3Linked to original sources
PubMed1995

Protection against chemotherapy-induced alopecia by cyclosporin A in the newborn rat animal model.

BACKGROUND: We have previously shown that several agents will protect against chemotherapy-induced alopecia in the newborn rat animal model. Such protective agents render the hair follicle keratinocytes resistant to chemotherapy possibly by the expression of P-glycoprotein (Pgp). Cyclosporin A (CSA) is a potent inhibitor of Pgp. METHODS AND RESULTS: Efficacy of CSA was tested in its effects on chemotherapy-induced alopecia in the newborn rat animal model. CSA, when applied topically, protected rats from local alopecia induced by various agents. CONCLUSION: The mechanism of protection by CSA and its relationship to Pgp remain uncertain. The potential clinical applicability of this observation remains to be determined.

Administration, Cutaneous↗
PubMed1995

Hemispheric picture-naming hierarchies in stuttering subjects.

The present study was done to investigate the linguistic organization of the right hemisphere of stuttering subjects and the interhemispheric interactions that underlie verbal output in this population. Naming reaction times of 14 stuttering adults were measured to unilaterally presented pictures corresponding to vocabulary levels of < 5.5, 9.5-10.5, and > 18.0 years of age. An analysis of variance of latencies showed a significant main effect for picture vocabulary-age. Post hoc tests were interpreted as suggesting that the right hemisphere of stuttering subjects was capable of differential picture-encoding operations in a manner similar to the left hemisphere of normal speakers. Also, naming latencies favored left visual-field stimulations by 34 msec. Taken with significant and high correlations between visual fields for each level of picture vocabulary score, the right hemispheres of the stuttering subjects appeared responsible for picture-encoding operations. Left-hemispheric stimulus processing was not predicted, suggesting differences may exist in interhemispheric interactions underlying picture-naming functions in stuttering populations.

Adult↗
PubMed1995

Cytokines in proliferative diabetic retinopathy and proliferative vitreoretinopathy.

We determined whether interleukin-8, monocyte chemotactic protein-1, and macrophage-colony stimulating factor are present in the vitreous of patients with proliferative diabetic retinopathy (PDR) or proliferative vitreoretinopathy (PVR). The levels of these cytokines were measured by specific enzyme-linked immunoassays in vitreous from 30 patients with PDR, 13 patients with PVR, and 26 control individuals, including 10 cadaver eyes and 16 patients with idiopathic macular holes, idiopathic macular puckers, vitreous hemorrhages, or uncomplicated retinal detachments. Detectable levels of interleukin-8 were found in 90% of vitreous samples of patients with PDR, 85% with PVR, and 58% of control samples. IL-8 was significantly increased in PDR (mean +/- SEM; 25.0 +/- 5.3 ng/ml; p = 0.01), but not in PVR (11.9 +/- 3.9 ng/ml; p = 0.50) compared to control human vitreous (8.5 +/- 2.5 2.5 ng/ml). MCP-1 was detected in 90% of vitreous samples of patients with PDR, 92% with PVR, and 81% of control samples. MCP-1 was significantly increased in PDR (6.2 +/- 0.9 ng/ml, p = 0.001) and PVR (7.7 +/- 2.5 ng/ml, p = 0.001) over the levels in control vitreous (1.2 +/- 0.2 ng/ml). M-CSF was detected in 94% of vitreous samples of patients with PDR, 88% with PVR, and 92% from control vitreous. M-CSF was significantly elevated in PDR (32.3 +/- 8.3 ng/ml, p = 0.03), but not in PVR (23.6 +/- 12.8 ng/ml, p = 0.4) compared to control (10.7 +/- 3.5 ng/ml). Our results suggest that IL-8, MCP-1, and M-CSF participate in the pathogenesis of PDR and PVR.

Chemokine CCL2↗
PubMed1995

Word recognition performance in continuous and interrupted broad-band noise by normal-hearing and simulated hearing-impaired listeners.

Word recognition performance was investigated in 12 normal-hearing young adults in continuous and interrupted broad-band noise as a function of signal-to-noise ratio (S:N) with and without a simulated high frequency hearing loss (i.e., low-pass filtered at 2000 Hz). Subjects exhibited conventional sigmoid performance-intensity functions in continuous noise, for both unfiltered and filtered conditions. In contrast, subjects demonstrated shallower performance-intensity functions in the interrupted noise conditions with overall superior performance under adverse signal-to-noise ratios relative to the continuous noise conditions. Separate two-way analyses of variances investigating mean word recognition performance differences as a function of normal listening (unfiltered) versus the simulated hearing loss (filtered). Signal-to-noise ratio for both continuous and interrupted noise conditions revealed a significant main effects for S:N with both noise conditions (p < .05) and a significant main effect for the simulated hearing loss only in the interrupted noise condition (p < .05). It was hypothesized that subjects' diminished performance in the interrupted noise condition with the stimulated high frequency hearing loss reflected a reduced ability to temporally resolve auditory information between the gaps of noise.

Adult↗
PubMed1994

Applicability of standard irregular field calculations for high-energy photon beams.

Irregular-field calculations are usually based on a sector-summation technique. This technique typically involves separating the dose into primary and scatter components. While the conceptual basis for this procedure is valid at lower energies, it becomes suspect at higher energies. However, despite this limitation, a straightforward application of the technique yields good accuracy for 18 MV X-rays if certain limitations are observed. This work describes the calculational formalism used and the approximations made. Computed results are compared with measurements. The levels of uncertainty in both are discussed.

Humans↗
PubMed1994

Effect of high-pass filtering on the neonatal auditory brainstem response to air- and bone-conducted clicks.

The effect of standard high-pass analog filtering on the neonatal auditory brainstem response (ABR) to air- and bone-conducted clicks at low intensity screening levels was investigated. Simultaneous three channel recorded ABRs were obtained from 20 neonates with filter settings of 30-3000, 100-3000, and 150-3000 Hz at intensity levels of 20, 30, and 40 dB nHL. Statistically significant reductions in wave V amplitude and decreases in wave V latency were observed for both transducers across all three low level stimulus intensities with the progressive increase in the high-pass filter cutoff (p < .05). These data support the advocacy of less restrictive high-pass filtering (e.g., 30 Hz) for neonatal and infant ABR screening to air- and bone-conducted clicks.

Adult↗
PubMed1994

Effect of frequency-altered feedback on stuttering frequency at normal and fast speech rates.

The purpose of this study was to determine the effect of the magnitude and direction of the frequency shift of frequency-altered auditory feedback (FAF) on stuttering frequency at both normal and fast speech rates. Twelve adult male and 2 adult female subjects who stutter read 10 different passages at either a normal or fast speech rate under nonaltered auditory feedback (NAF) and each of four FAF conditions in which the feedback signal was shifted: up one-half octave; up one octave; down one-half octave; and down one octave. Mean stuttering frequency for NAF was significantly higher than mean stuttering frequencies for all FAF conditions (p < 0.05). There were no statistically significant differences between the FAF conditions (p > 0.05). Subjects exhibited significantly more disfluencies under the fast speech rate condition relative to the normal speech rate condition. Future research should examine the relationship between frequency shifts of less than one-half octave and stuttering amelioration.

Adolescent↗
PubMed1994

Neonatal auditory brainstem response thresholds to air- and bone-conducted clicks: 0 to 96 hours postpartum.

Auditory brainstem response (ABR) thresholds to air- and bone-conducted clicks were investigated in 40 full-term neonates. Subjects were divided into two groups of 20 according to postpartum age: less than 48 hours and between 49 and 96 hours. Mean ABR thresholds to air- and bone-conducted clicks for neonates less than 48 hours postpartum were 14.5 dB nHL (51.5 dB peak SPL) and 1.8 dB nHL (36.8 peak re: 1 microN), respectively, while those for neonates between 49 and 96 hours were 3.8 dB nHL (40.8 dB peak SPL) and 1.5 dB nHL (36.5 dB peak re: 1 microN), respectively. A significant difference was found between the two group mean ABR thresholds to air-conducted stimuli (p < .0001) but not for the bone-conducted stimuli (p < .8959). A statistically significant within-group difference was found between the ABR thresholds to air- and bone-conducted stimuli for only the neonates less than 48 hours of age (p < .0001). When the data was collapsed across groups, simple linear regression analyses revealed a statistically significant relation between postpartum age and ABR threshold to air-conducted stimuli (p < .0001) and a nonsignificant relation between postpartum age and ABR threshold to bone-conducted stimuli (p < .9744). These findings support the notion that some resolution of fluids and residuals in the middle ear occurs during the first 48 hours postpartum and that air-conducted stimuli are attenuated during that period. As such, a physiologic conductive deficit among the younger neonates is suggested.

Acoustic Stimulation↗
PubMed1993

Auditory brain stem responses to air- and bone-conducted clicks in the audiological assessment of at-risk infants.

Auditory brain stem responses (ABRs) to air- and bone-conducted clicks were used to assess the auditory status of 170 at-risk neonates. During the perinatal period, 20.6% (35/170 cases) of the at-risk infants failed ABRs to air-conducted clicks at 30 dB nHL in at least one ear. Ear-specific results indicated an initial failure rate of 15.0% (51/340 ears). Approximately two-thirds (32/51 ears) of these initial failures showed purely conductive deficits, whereas the remaining one-third (19/51 ears) involved suspected sensorineural components. Follow-up audiological evaluations were performed for 87.1% (148 cases) of these at-risk infants at 4 mo and/or 1 yr corrected age. Based on the initial tests and follow-up assessments, the tentative operating characteristics of ABRs to both air- and bone-conducted clicks for identification of sensorineural deficits in at-risk neonates were calculated. It was found that the ABR to bone-conducted clicks yielded better specificity, predictive value of positive results, and overall efficiency. It is suggested that the ABR to bone-conducted stimuli should be viewed as a valuable addition in the assessment of cochlear reserve in infants who fail a newborn auditory screening to air-conducted stimuli.

Acoustic Stimulation↗
PubMed1993

Depressive symptoms in blood donors notified of HIV infection.

OBJECTIVES: Understanding more about the psychological state of persons notified of human immunodeficiency virus (HIV) infection is critical for designing notification and counseling programs that will have the most positive effect. METHODS: The subjects were blood donors who had been notified of HIV infection by the New York Blood Center. A nurse elicited a medical history, performed a limited medical examination, and asked the subjects to complete a questionnaire that included questions about drug use, sexual behavior, and psychological characteristics. The subjects completed another questionnaire approximately 2 weeks later. RESULTS: The average depressive symptom scores for both men and women were substantially higher than scores typically found in representative population samples. More than a quarter of the men and more than a third of the women reported seeking psychological or psychiatric services in the first few weeks following notification. CONCLUSIONS: Anticipating and meeting individuals' psychological needs may be necessary if HIV screening programs are to address effectively the needs of persons infected with HIV.

Adaptation, Psychological↗
PubMed1993

Test-retest variability of the auditory brainstem response to bone-conducted clicks in newborn infants.

The variability of the auditory brainstem response (ABR) to bone- and air-conducted clicks was investigated utilizing a test-retest paradigm with 20 normal full-term newborn infants. ABRs to bone-conducted clicks at 15 and 30 dB nHL and air-conducted clicks at 30 dB nHL were obtained. The delivery of the bone-conducted signal was controlled. ABR wave V latencies were measured from each test-retest stimulus condition. The results indicated no statistically significant difference in test-retest variability of ABR wave V latencies or amplitudes between bone- and air-conducted clicks. ABRs to bone-conducted clicks, under controlled clinical conditions, are as reproducible and reliable as ABRs to air-conducted clicks in newborn infants.

Acoustic Stimulation↗
PubMed1993

Auditory brainstem response thresholds to air and bone conducted clicks in neonates and adults.

Auditory brainstem response (ABR) thresholds to air and bone conducted clicks were investigated in 20 full-term neonates and 20 normal-hearing young adults. Results showed mean ABR thresholds to air and bone conducted clicks for neonates to be 3.75 dB nHL (40.75 peak SPL) and 1.25 dB nHL (36.25 dB peak re: 1 microN), respectively, and for adults 3.75 dB nHL (40.75 peak SPL) and 18.75 dB nHL (53.75 peak re: 1 microN), respectively. A significant difference was observed in mean ABR thresholds to bone conducted stimuli between neonates and adults (p < 0.0001) and among mean adult ABR thresholds to air and bone conducted stimuli (p < 0.0001). Nonsignificant differences were observed in mean ABR thresholds to air and bone conducted stimuli among neonates (p > 0.05) and in mean ABR thresholds to air conducted stimuli between neonates and adults (p = 1.00). It is speculated that the dissimilarity in the relationship of thresholds to air and bone conducted stimuli between neonates and adults reflects, in part, a difference in efficiency of signal delivery to the cochlea.

Acoustic Stimulation↗
PubMed1992

Modulation of macrophage colony stimulating factor in cultured human retinal pigment epithelial cells.

Steady-state mRNA expression and protein production of macrophage colony stimulating factor were measured in visually confluent monolayers of unstimulated cultured human retinal pigment epithelial cells and after cells were stimulated with recombinant cytokines. Using reverse transcription polymerase chain reaction, macrophage colony stimulating factor mRNA expression was detected in unstimulated cells obtained from each of four separate donors. In these cells, mRNA expression was accompanied by secretion of macrophage colony stimulating factor protein into cell-conditioned medium; 48 hr after cells were switched to fresh medium, the mean (+/- S.D.) quantity of macrophage colony stimulating factor, measured by enzyme-linked immunoassay, was 5.1 +/- 2.3 ng 10(-6) cells. There was a dose- and time-dependent induction of macrophage colony stimulating factor mRNA after cells were exposed to recombinant human interleukin-1 and tumor necrosis factor alpha. Maximal mRNA induction was observed in cells exposed for 4 hr to interleukin-1 beta (5 U ml-1) or for 4-8 hr to tumor necrosis factor alpha; under these conditions, macrophage colony stimulating factor mRNA was induced up to 23- and 46-fold after exposure to interleukin-1 beta and tumor necrosis factor alpha, respectively. Similarly, macrophage colony stimulating factor protein production was enhanced after cells were exposed to recombinant cytokines. Protein secretion increased 1.3-2.5-fold (P less than 0.001) after exposure to interleukin-1 beta (5 U ml-1), and 1.2-1.6-fold after exposure to tumor necrosis factor alpha (P less than 0.03).(ABSTRACT TRUNCATED AT 250 WORDS)

Cells, Cultured↗
PubMed1992

Expression of interleukin-1 alpha, interleukin-1 beta, and an interleukin-1 receptor antagonist in human retinal pigment epithelial cells.

mRNA expression and protein production of interleukin-1 alpha, interleukin-1 beta and intracellular and secreted forms of an interleukin-1 receptor antagonist were measured in visually confluent monolayers of unstimulated cultured human retinal pigment epithelial cells and after cells were stimulated with recombinant cytokines. Using reverse transcription polymerase chain reaction, transcripts for interleukin-1 alpha and interleukin-1 beta were not detected in unstimulated cells from any of six donors whereas mRNA expression for both interleukin-1 alpha and interleukin-1 beta was readily induced in all six cell lines after cells were stimulated with recombinant IL-1 (alpha or beta), tumor necrosis factor alpha, or lipopolysaccharide. The combination of cycloheximide and recombinant interleukin-1 caused a 14-fold enhancement of interleukin-1 alpha and interleukin-1 beta mRNA expression above that observed after cells were stimulated with interleukin-1 alone. After stimulation by interleukin-1, cells produced intracellular interleukin-1 alpha protein, but did not secrete it into medium. In contrast, interleukin-1 beta protein was not detected in cell lysates or conditioned-medium after stimulation with interleukin-1. An intracellular interleukin-1 receptor antagonist was expressed constitutively by human retinal pigment epithelial cells; mRNA transcripts were enhanced in a dose and time dependent manner after cells were exposed to recombinant interleukin-1 or tumor necrosis factor alpha. In contrast, mRNA for a secreted form of the interleukin-1 receptor antagonist was not detected under basal conditions or after cells were stimulated by recombinant cytokines. Interleukin-1 receptor antagonist protein was found primarily in cell lysates; little interleukin-1 receptor antagonist protein was secreted by the cells. The presence of cell-associated interleukin-1 receptor antagonist was confirmed by immunocytochemistry. Levels of cell-associated IL-1 receptor antagonist protein were not significantly influenced by recombinant interleukin-1 or tumor necrosis factor alpha. Endogenous expression of interleukin-1 receptor antagonist may attenuate the effect of exogenous or endogenous interleukin-1, thus providing the RPE cell a means of maintaining interleukin-1 homeostasis in ocular inflammatory disease.

Cells, Cultured↗
PubMed1991

HIV-2 infection in an American.

HIV-2 is endemic in West Africa but rare elsewhere. In the USA there have been 18 reported cases of HIV-2 infection; most identified people have been West Africans. We recently diagnosed the first case of HIV-2 infection in a native-born US citizen, a woman whose serum was found to be reactive to anti-HIV-1 enzyme immunoassay (EIA) when she attempted to donate blood in 1986. Although both HIV-1- and HIV-2-specific EIAs were reactive, the anti-HIV-2 Western blot (WB) was positive, while the anti-HIV-1 WB was positive or indeterminate on different occasions. Synthetic peptide testing was reactive for HIV-2 but not HIV-1. HIV-2 DNA was detected using the polymerase chain reaction procedure. Although she had travelled to West Africa, it is unclear how she became infected with HIV-2.

Adult↗
PubMed1991

Effect of vibrator to head coupling force on the auditory brain stem response to bone conducted clicks in newborn infants.

The effect of vibrator to head coupling force on the auditory brain stem response (ABR) to bone conducted clicks in newborn infants was investigated. Twenty full term newborn infants were tested. ABRs to bone conducted clicks were obtained with four different coupling forces (225, 325, 425, and 525 g) at stimulus intensities of 15 and 30 dB nHL. ABRs to air conducted clicks were also obtained at 30 dB nHL. The results of this study indicated that ABR wave V latencies to bone conducted clicks in newborn infants were affected significantly when the vibrator to head coupling force shift exceeded 200 g. It is recommended that the coupling force be controlled and remain consistent when implementing ABR to bone conducted stimuli in newborn infants.

Audiometry, Evoked Response↗
PubMed1991

Probe tube microphone measures of loudness discomfort levels in children.

Loudness discomfort levels (LDLs) have been advocated as a means for selecting the SSPL90 setting of an individual's hearing aid. Kawell, Kopun, and Stelmachowicz (Ear Hear 1988; 9: 133-136) recently developed a procedure to measure LDLs in children. Several procedural cautions, involving the hearing aid stimulus delivery and sound field calibration, have been noted with this method. As a means of overcoming these problems, a new method utilizing insert earphone derived stimuli delivered to a child's ear-mold with probe tube microphone monitoring of real-ear sound pressure level was explored. Twenty children, aged 7 to 14 years, served as subjects. The advantage of the present method lies in the procedural conveniences and the ability to compare real-ear audiometric measures and hearing aid performance.

Adolescent↗